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Antibody diluent for enhancing immune imprinting detection signal

A technology for antibody diluent and signal detection, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of antigen or antibody cross-contamination, no signal enhancement, and low signal-to-noise ratio, so as to avoid non-specific binding and enhance Intensity and Sensitivity, Effect of Strong Signal-to-Noise Ratio

Inactive Publication Date: 2019-10-01
武汉博士德生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The antibody diluent used in the existing immunoblotting detection is usually prepared from proteins such as bovine serum albumin (BSA), skim milk, casein, animal gelatin, etc., which can easily lead to cross-contamination of antigens or antibodies, resulting in a high background. The signal-to-noise ratio is relatively low, and there is no signal enhancement

Method used

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  • Antibody diluent for enhancing immune imprinting detection signal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Prepare 1 L of TBS buffer solution with a concentration of 0.02 mol / L and a pH of 7.2 to 7.6. The specific preparation method is as follows: Weigh 2.42 g of Tris and 5.84 g of NaCl and dissolve them in 800 mL of distilled water, adjust the pH to 7.2 to 7.6 with HCl, and then Add distilled water to make up to 1L.

[0014] Take 800mL prepared TBS buffer solution, add 1g polyvinyl alcohol, 0.5g polyvinylpyrrolidone and 1g alkyl glucoside, stir evenly, measure 1mL Cathone solution with a concentration of 15%, add it to 800mL TBS buffer solution, Then dilute to 1 L with TBS buffer.

Embodiment 2

[0016] Prepare 1 L of TBS buffer solution with a concentration of 0.01 mol / L and a pH of 7.2 to 7.6. The specific preparation method is as follows: Weigh 1.21 g of Tris and 5.84 g of NaCl and dissolve them in 800 mL of distilled water, adjust the pH to 7.2 to 7.6 with HCl, and then Add distilled water to make up to 1L.

[0017] Take 800mL prepared TBS buffer solution, add 0.25g polyvinyl alcohol, 1g polyvinylpyrrolidone and 0.1g alkyl glucoside, stir evenly, measure 0.1mL Cathone solution with a concentration of 15%, add 800mL TBS buffer solution, and then dilute to 1 L with TBS buffer.

Embodiment 3

[0019] Prepare 1 L of TBS buffer solution with a concentration of 0.1 mol / L and a pH of 7.2 to 7.6. The specific preparation method is as follows: Weigh 12.1 g of Tris and 5.84 g of NaCl and dissolve them in 800 mL of distilled water, adjust the pH to 7.2 to 7.6 with HCl, and then Add distilled water to make up to 1L.

[0020] Take 800mL prepared TBS buffer solution, add 1g polyvinyl alcohol, 2.5g polyvinylpyrrolidone and 1g alkyl glucoside, stir evenly, measure 5mL Cathone solution with a concentration of 15%, add it to 800mL TBS buffer solution, Then dilute to 1 L with TBS buffer.

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Abstract

The invention relates to an antibody diluent for enhancing an immune imprinting detection signal. Each 1L of antibody diluent comprises the following components in percentage by weight: 0.25-2.5g of polyvinyl alcohol, 0.25-2.5g of polyvinylpyrrolidone, 0.1-2g of surfactant, 0.015-0.75mL of Kathon preservative and the balance of 0.01-0.1moL / L TBS buffer solution, wherein the pH of the TBS buffer solution is 7.2-7.6. The antibody diluent for enhancing the immune imprinting detection signal can be used for antibody dilution of immune imprinting detection, so that a relatively high background generated by pollution caused by protein is avoided; a relatively strong signal-to-noise ratio is displayed; and meanwhile, the strength and the sensitivity of the immune imprinting signal are enhanced.

Description

technical field [0001] The invention relates to the technical field of western blot detection, in particular to an antibody diluent for enhancing the detection signal of western blot. Background technique [0002] Western blot detection is a commonly used test method in life science research such as molecular biology, biochemistry and immunogenetics. Its principle is to distinguish the different components of the sample to be tested by SDS-polyacrylamide gel electrophoresis, and then in the Under the action, the protein is transferred from the gel to the blotting membrane, and the target antigen protein is detected by using the specific antibody as a probe, and the position and intensity of the specific reaction can be obtained by analyzing the position and intensity of the specific protein in the analyzed cells and / or tissues. In the process of Western blot detection, DAB color development, ECL chemiluminescent substrate color development, fluorescently labeled secondary an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 夏一方陈莉胡方影胡良波乐杨静
Owner 武汉博士德生物工程有限公司
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