Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of rapid detection test paper and its preparation method and application

A detection test strip and rapid technology, applied in the field of immunochemical in vitro detection, can solve the problems of wrong judgment of results and no color development of the quality control line, and achieve the effects of increasing repeatability, overcoming reflux, and avoiding non-specific binding.

Active Publication Date: 2021-06-11
RUNBIO BIOTECH CO LTD
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a rapid detection reagent, under normal circumstances, the quality control line must develop color after the test is completed. The control line does not show color, which leads to misjudgment of the result

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of rapid detection test paper and its preparation method and application
  • A kind of rapid detection test paper and its preparation method and application
  • A kind of rapid detection test paper and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] This embodiment provides a kind of early pregnancy detection test strip, the structure is as follows: figure 1 with figure 2 As shown, the state of contact between the chromatographic membrane and the binding pad is as follows Image 6 As shown, it includes a bottom plate 1 , a chromatographic membrane 2 , a binding pad 3 , and a single-sided adhesive tape 11 , wherein there are two lines on the chromatographic membrane: a detection line 5 and a quality control line 6 . The specific preparation method of the detection test strip is as follows:

[0039] 1. Preparation of conjugate pad (3) containing conjugate (4)

[0040] 1.1 Preparation of quality control line (6) microsphere conjugate (4-2)

[0041] In this product, the antibody labeled by the quality control line is: rabbit IgG antibody, and the microspheres used for labeling are: blue microspheres.

[0042] 1.1.1 Microsphere cleaning

[0043] Take 1 mL of blue microspheres in a beaker, weigh 16 mL of reaction b...

Embodiment 2

[0091] This embodiment provides a cardiac troponin I / creatine kinase isoenzyme / myoglobin simultaneous detection reagent card, the structure of which is as follows: image 3 As shown, it includes a chromatographic membrane 2, a binding pad 3 pasted on the bottom plate (1), and a box-type cartridge 12-1. Wherein there are 3 detection lines (5) (5a-cardiac troponin I, 5b-creatine kinase isoenzyme, 5c-myoglobin) and quality control line (6) on the chromatographic membrane 2; ) on the conjugate (4), the conjugate (4) is a mixture of microspheres of different colors, including 4-1a cardiac troponin I, 4-1b creatine kinase isoenzyme, 4-1c myoglobin , 4-2 rabbit IgG. The specific preparation method of the detection kit is as follows:

[0092] 1. Preparation of conjugate pad (3) containing conjugate (4)

[0093] 1.1 Quality control line (6) Preparation of microsphere conjugate 4-2

[0094] The quality control line microspheres are labeled with black microspheres, and the labeled an...

Embodiment 3

[0140] This embodiment provides a multi-drug detection test strip, the structure of which is as follows: Figure 4 As shown, it includes a bottom plate (1), a chromatographic membrane (2), a binding pad (3), and a single-sided adhesive tape (11). Wherein the detection line (5) and the quality control line (6) are arranged on the chromatographic membrane (2), and the conjugate (4) is arranged on the binding pad (3), and each test strip all contains the quality control line (6), and the detection The area of ​​the line (5) is coated with different antigens, up to 5, at intervals of 2.5 mm. The specific preparation method of the drug detection quilt is as follows:

[0141] 1. Preparation of conjugate pad (3) containing conjugate (4)

[0142] 1.1 Preparation of quality control line (6) microsphere conjugate (4-2)

[0143] The microspheres used for quality control markers are yellow microspheres.

[0144] 1.1.1 Microsphere cleaning

[0145] Take 1 mL of yellow microspheres in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a rapid detection test paper and its preparation method and application. The test paper includes a chromatographic membrane and a binding pad, one end of the chromatographic membrane is connected to the binding pad; the binding pad is pre-sprayed with microsphere marks of different colors Different analytes correspond to antigen / antibody conjugates, and the chromatographic membrane is provided with a detection line and a quality control line in sequence along the direction of liquid chromatography, and the detection line is the antigen / antibody corresponding to different analytes fixed at a certain interval. . The invention also provides the application of the detection test paper in the detection of drugs and early pregnancy.

Description

technical field [0001] The invention belongs to the technical field of immunochemical in vitro detection, and in particular relates to a novel rapid detection test paper and its preparation method and application Background technique [0002] In recent years, with the development of science and technology and the advancement of medical science, as well as efficient and fast-paced working methods, POCT, which has the advantages of miniaturization of experimental instruments, simple operation, and instant report results, has become more and more popular. [0003] On-site rapid testing, also called point-of-care testing, referred to as POCT, refers to a testing method that is carried out at the sampling site and uses portable analytical instruments and supporting reagents to quickly obtain test results. Or all tests performed outside the central laboratory. [0004] POCT is a new field for the development of laboratory medicine, and it is also one of the most promising fields ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/76G01N33/53G01N33/558
CPCG01N33/543G01N33/558G01N33/68G01N33/76G01N33/948G01N2333/59
Inventor 高克谨林朝琨
Owner RUNBIO BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com