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Dialysis device and silicon nanowire field-effect tube combined biosensor

A technology of biosensors and silicon nanowires, applied in measuring devices, biological testing, instruments, etc., can solve the problems of high requirements, strong detection subjectivity, and low sensitivity

Active Publication Date: 2018-12-07
WUXI PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the widely used clinical tumor marker detection method is the classic ELISA method, but its clinical application is limited to some extent due to its high requirements for the detection environment, strong detection subjectivity, and low sensitivity.

Method used

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  • Dialysis device and silicon nanowire field-effect tube combined biosensor
  • Dialysis device and silicon nanowire field-effect tube combined biosensor
  • Dialysis device and silicon nanowire field-effect tube combined biosensor

Examples

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Effect test

Embodiment 1

[0066] Such as figure 1 , 2 , shown in 3, 4, a kind of biosensor of joint dialysis device and silicon nanowire field effect tube, described sensor comprises dialysis device 20, silicon nanowire field effect tube 22 and signal output computer 24, and described dialysis device 20 passes The thin silicone hose 11 communicates with the silicon nanowire field effect tube 22; the silicon nanowire field effect tube 22 transmits the signal to the signal output computer 24 through the probe 23.

[0067] The dialysis device 20 is composed of three-in-one dialysis tubing 13 placed in deionized water 12 .

[0068] Described silicon nanowire field effect transistor 22 comprises silicon nanowire integrated circuit chip and PMDS microfluidic pipe 10; Described silicon nanowire integrated circuit chip comprises back gate 7 and the insulating silicon sheet lining that is tiled on back gate 7 tops Bottom 6, the insulating silicon wafer substrate 6 is provided with three parallel electrodes, ...

Embodiment 2

[0095] Example 2: Trace and real-time detection of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA):

[0096] The detection method comprises the following steps:

[0097] (1) For the tumor markers to be detected, use the APTES / glutaraldehyde chemical bond to connect the relevant antibody to the antigen to be detected, and reversibly seal the PDMS microchannel and the silicon nanowire integrated circuit chip, and connect it into the entire In the detection system, a flow path is formed;

[0098] (2) Start the whole integrated device, set the voltage of the source and drain to 2V and 0V respectively; set the voltage of the top grid to 2V, and set the voltage of the back grid to -0.8V; the peristaltic pump is the whole integrated equipment The power system, the flow rate in the flow path is 80ul / min, the time to pass the test solution is 3min, the total flux of the test solution is about 240ul, the serum sample first passes through the dialysis equipment, and when it p...

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Abstract

The invention relates to a dialysis device and silicon nanowire field-effect tube combined biosensor. The dialysis device and silicon nanowire field-effect tube combined biosensor comprises a dialysisdevice, a silicon nanowire field-effect tube and a signal output computer, wherein the dialysis device is communicated with the silicon nanowire field-effect tube through a thin silica gel flexible pipe; and the silicon nanowire field-effect tube transmits a signal to the signal output computer through a probe. The serum desalting dialysis device and the silicon nanowire field-effect tube are connected into the same pipeline; the dialysis device is used as a filter; the field-effect tube having a double gate structure with a semiconductor silicon nanowire as a channel is used as a signal converter; an antibody corresponding to a tumor marker is used as a biological sensitive element; and therefore, the immediate, label-free and high-sensitivity detection of the serum tumor marker is realized.

Description

technical field [0001] The invention relates to the technical field of biological material detection, in particular to a biosensor for joint detection of tumor markers using devices such as silicon nanowire field effect tubes. Background technique [0002] Malignant tumors are currently one of the major diseases that threaten human health, but most of the tumors are in the advanced stage of clinical manifestations, so early, rapid and sensitive diagnosis of them is an important way to improve the quality of human life. At present, the monitoring methods of clinical tumor incidence mainly rely on imaging examination and tumor marker detection. Imaging examinations often do not allow long-term follow-up due to resolution and radiation risks. Although the detection of tumor markers is relatively simple to operate, the sensitivity and specificity of tumor marker detection have become important factors that limit its application. Therefore, finding a simple and accurate detecti...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57484G01N33/6854
Inventor 王彤张烨陈航李增耀
Owner WUXI PEOPLES HOSPITAL
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