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A kind of surface modification method of qcm wafer

A surface modification and wafer technology, applied in the field of biosensors, can solve the problems affecting the accuracy of measurement, adverse reactions, changes, etc., and achieve the effects of improving accuracy, good biocompatibility, and high sensitivity

Active Publication Date: 2021-11-16
南通市产品质量监督检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing wafer materials often have microscopic processes such as non-specific attachment, agglomeration, and repulsion of biomass in biological media, resulting in changes in the energy, frequency, and phase of oscillations and interference, which is the so-called Missing Mass ) effect adverse reactions, affecting the accuracy of measurement

Method used

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  • A kind of surface modification method of qcm wafer
  • A kind of surface modification method of qcm wafer
  • A kind of surface modification method of qcm wafer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0021] The surface modification method of the present embodiment QCM wafer, this surface modification method comprises the steps:

[0022] Step 1: Prepare 50 mL of ethanol solution containing 1% by mass of ethyl 3-mercaptopropionate, put 12 gold-plated QCM chips into 12-well culture plates, add 3 mL of the above solution to each well, and soak for 1 h Finally, remove the solution, and then use 95% ethanol to clean the surface of the wafer and wait for it to dry naturally;

[0023] Step 2: prepare 20mL polymer A mass percent is 0.5% ethanol solution, wherein the structural formula of polymer A is

[0024]

[0025] Place the QCM wafer processed in step 1 on a glass slide, add the ethanol solution of the polymer dropwise to completely cover the wafer surface, use a mask with a grid width and spacing of 0.1mm to cover the wafer surface, and use a power of 60mW and a wavelength of 250nm ultraviolet light is irradiated for 1 minute to bond the polymer to the surface of the wafer...

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Abstract

The invention relates to a method for modifying the surface of a QCM wafer. The gold-plated QCM wafer is soaked with an ethanol solution of ethyl 3-mercaptopropionate, the surface is cleaned with ethanol and air-dried naturally; The treated QCM wafer is infiltrated with the substance solution, and the wafer surface is covered with a strip or grid mask. Under the induction of ultraviolet light, the polymer is bonded to the wafer surface to form an array structure; it is washed successively with absolute ethanol and deionized water. On the surface of the wafer, remove the unreacted bonded polymer; use the buffer solution containing the antibody to soak the wafer, use deionized water to clean the wafer, and store the wafer in the buffer solution for future use. The advantage of the present invention is that: a polymer film with a certain pattern structure is prepared on the surface of the QCM wafer by light-induced grafting technology, and antibody molecules are further grafted on the surface of the film to form a "antibody-antigen reaction" that can capture specific biomolecules. Antenna" array with high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of biosensors, in particular to a method for modifying the surface of a QCM chip. Background technique [0002] Biosensor is an important tool in the field of food detection. It realizes the analysis and monitoring of target components by combining biometric components and signal conversion components. It has good selectivity, no reagents, easy operation, reusability and Online analysis and other advantages. In recent years, a variety of biosensors based on different principles have been developed and used to detect harmful ingredients in food. Among them, a technology that combines the principle of immunity with Quartz Crystal Microbalance (QCM) has received extensive attention. [0003] QCM utilizes the piezoelectric effect of quartz crystal to convert the change of the surface quality of the quartz crystal electrode into the frequency change of the output electrical signal of the quartz crystal oscillato...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N5/00
CPCG01N5/00G01N33/5302
Inventor 陈刚邵彪李子寅王彦军
Owner 南通市产品质量监督检验所
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