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Method for inhibiting non-specific adsorption of solid material surface

A solid material, non-specific technology, applied in the direction of surface coating liquid devices, special surfaces, material inspection products, etc., can solve the problems of reducing non-specific adsorption of biomolecules, adsorption reduction, complex structure, etc., to achieve rapid preparation, The effect of easy operation and wide application prospects

Inactive Publication Date: 2012-04-25
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are also carboxyl polymers as non-specific inhibitory surfaces, but this polymer structure is relatively complex and difficult to prepare (Langmuir2007, 23, 5670-5677)
However, the currently reported methods all reduce the non-specific adsorption of biomolecules on the surface of the modified solid material to some extent, but cannot reduce the adsorption to zero or close to zero.

Method used

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  • Method for inhibiting non-specific adsorption of solid material surface
  • Method for inhibiting non-specific adsorption of solid material surface
  • Method for inhibiting non-specific adsorption of solid material surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] After 15 single crystal silicon wafers with silicon dioxide grown on the surface were cleaned with absolute ethanol and deionized water, the surface was cleaned in an oxygen plasma cleaner for 0.5 minutes to completely remove organic dirt. At 80°C, the wafers were divided into 5 groups and placed in perfluoropropyltriethoxysilane [CF 3 (CF 2 ) 2 Si(OCH 2 CH 3 ) 3 ], 1,1,2,2-tetrahydroperfluorohexyltriethoxysilane [CF 3 (CF 2 ) 3 (CH 2 ) 2 Si(OCH 2 CH 3 ) 3 ], 1,1,2,2,3,3,4,4,5,5-decahydroperfluorohexadecyltriethoxysilane [CF 3 (CF 2 ) 10 (CH 2 ) 5 Si(OCH 2 CH 3 ) 3 ], 1,1,2,2-tetrahydroperfluorooctylmethoxy-diethoxysilane [CF 3 (CF 2 ) 5 (CH 2 ) 2 SiOCH 3 (OCH 2 CH 3 ) 2 ], 1,1,2,2-tetrahydroperfluorooctyldiethoxychlorosilane [CF 3 (CF 2 ) 5 (CH 2 ) 2 Si(OCH 2 CH 3 ) 2 Cl] in the gas phase atmosphere for 24 hours, after taking it out, use absolute ethanol to clean the surface, and dry it with nitrogen flow for later use. Through the ...

Embodiment 2

[0029] After cleaning 15 monocrystalline silicon wafers with silicon dioxide grown on the surface with absolute ethanol and deionized water, the surface was cleaned in an oxygen plasma cleaner for 3 minutes to completely remove organic dirt. At 150°C, the wafers were divided into 5 groups and placed in perfluoropropyltrimethoxysilane [CF 3 (CF 2 ) 2 Si(OCH 3 ) 3 ], 1,1,2,2-tetrahydroperfluorohexylmethoxysilane [CF 3 (CF 2 ) 3 (CH 2 ) 2 Si(OCH 3 ) 3 ], 1,1,2,2,3,3,4,4,5,5-decahydroperfluorohexadecylmethoxysilane [CF 3 (CF 2 ) 10 (CH 2 ) 5 Si(OCH 3 ) 3 ], 1,1,2,2-tetrahydroperfluorooctyldimethoxychlorosilane [CF 3 (CF 2 ) 5 (CH 2 ) 2 Si(OCH 3 ) 2 Cl], 1,1,2,2-tetrahydroperfluorooctyldimethoxyisocyanosilane [CF 3 (CF 2 ) 5 (CH 2 ) 2 Si(OCH 3 ) 2 CNO] in the gas phase atmosphere for 10 seconds, after taking it out, use absolute ethanol to clean the surface, and blow dry with nitrogen flow for later use. Through the above method, a layer of fluorinate...

Embodiment 3

[0031] After cleaning 15 monocrystalline silicon wafers with silicon dioxide grown on the surface with absolute ethanol and deionized water, the surface was cleaned in an oxygen plasma cleaner for 3 minutes to completely remove organic dirt. At 150°C, the wafers were divided into 5 groups and placed in perfluoropropyltrichlorosilane [CF 3 (CF 2 ) 2 SiCl 3 ], 1,1,2,2-tetrahydroperfluorohexyltrichlorosilane [CF 3 (CF 2 ) 7 (CH 2 ) 2 SiCl 3 ], 1,1,2,2,3,3,4,4,5,5-decahydroperfluorohexadecyltrichlorosilane [CF 3 (CF 2 ) 10 (CH 2 ) 5 SiCl 3 ], 1,1,2,2-tetrahydroperfluorooctylmethoxydichlorosilane [CF 3 (CF 2 ) 5 (CH 2 ) 2 Si(OCH 3 )Cl 2 ], 1,1,2,2-tetrahydroperfluorooctylisocyanodichlorosilane [CF 3 (CF 2 ) 5 (CH 2 ) 2 Si(CNO)Cl 2 ] in the gas phase atmosphere for 10 seconds, after taking it out, use absolute ethanol to clean the surface, and blow dry with nitrogen flow for later use. Through the above method, a layer of fluorinated monomolecular layer fi...

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Abstract

The invention relates to a method for inhibiting non-specific adsorption of a solid material surface, in particular to a method for solving the problem of non-specific adsorption of large biological molecule, germ, cell and the like through combining the non-ionic surface active agent on fluoride monomolecular layer surface or nylation monomolecular layer surface. The invention comprises: a layerof fluoride monomolecular layer film or nylation monomolecular layer film is modified on the solid material surface, and when using mixed non-ionic surface active agent to prepare buffer solution containing large biological molecule, the large biological molecule can inhibit non-specific adsorption of large biological molecule in the buffer solution together with the mixed non-ionic surface active agent and the fluoride monomolecular layer film or nylation monomolecular layer film. Through the cooperation of non-ionic surface active agent, and fluoride surface or nylation surface, the invention can simply, effectively and fast prepare the large biological molecule microarray detection part, is easy to operate, has low cost and is applicable for mass production.

Description

technical field [0001] The invention relates to a method for inhibiting non-specific adsorption on the surface of solid materials, especially the use of non-ionic surfactants on the surface of fluorinated monomolecular layers or carboxylated monolayers to solve the problem of biological macromolecules, bacteria, cells, etc. Non-specific adsorption problem. Background technique [0002] Because the type of interaction between the surface of a solid material and the biological environment and the magnitude of the force depend on the nature of the surface of the material. These surface properties directly affect the adsorption of biological macromolecules such as proteins and nucleic acids on the surface. Among them, the non-specific adsorption of proteins is particularly noticeable. The adsorption of protein on the surface is considered to be the first reaction when the material is in contact with the biological environment, and it will affect a series of subsequent biologic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B05D5/08G01N33/48
Inventor 高云华胡科家陈志峰
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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