Superposition type fixed bed phase-conversion focusing chromatographic column and separation method

Abstract

The invention provides a superposition type fixed bed phase-conversion focusing chromatographic column and further provides a method which simultaneously separates lumbrukinase, superoxide dismutase and compound amino acid by adopting the same. The present status that single finished products are produced in the traditional biological pulp production line is reformed, and intensified continuous separation for a plurality of drug effect ingredients in the same column bed is realized under the premise that the economic accounting indicators, such as quality, yield, energy consumption, working hours and the like of the original biochemical products are kept at the original level.

Description

Technical field [0001] The invention relates to a superimposed fixed-bed phase-changing focusing chromatography column, and also relates to a method for simultaneously separating lumbrokinase, superoxide dismutase and compound amino acids by using the chromatography column, and belongs to the fields of biochemical separation media and biochemical pharmaceuticals. Background technique [0002] When modern biochemical technology extracts biological products on a large scale, it generally follows the principle of the only target substance, that is, the specific separation of a single substance. For example, earthworm body is a rich treasure house of natural medicine resources, lumbrokinase EK, superoxide dismutase SOD, and compound amino acid AA are the three effective components of earthworm body that are beneficial to the human body. In order to achieve their large-scale production requirements, separate designs are usually required. Three technological processes, three production...

AI Technical Summary

Problems solved by technology

[0009] In order to solve the problems existing in the above-mentioned prior art, the object of the present invention is to provide a superimposed fixed bed phase-changing focusing chromatography column and a separation method, which can change the status quo of a single finished product in the current bioslurry production line, while maintaining the quality of the original chemical product Under the premise of the original level of economic accounting indicators such as production, energy consumption, and working hours, the intensive and continuous separation of various medicinal components in the same column bed is realized.

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