Polydimethylsiloxane surface modification method based on hydrophobin/methylcellulose

A technology of polydimethylsiloxane and methylcellulose, which is applied in the field of surface modification of polydimethylsiloxane microfluidic chips, can solve problems such as unstable physical adsorption coatings, and achieve high efficiency The effects of reproducible separation, suppression of non-specific adsorption, and improvement of chromatographic peak shape

Inactive Publication Date: 2014-05-07
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the disadvantage of unstable physical adsorption coating existing in the surface modification method of the existing microfluidic chip, and to provide a polymer with strong hydrophilicity on the surface of the chip, high anti-pollution ability and stable coating. Surface modification method of dimethylsiloxane microfluidic chip

Method used

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  • Polydimethylsiloxane surface modification method based on hydrophobin/methylcellulose
  • Polydimethylsiloxane surface modification method based on hydrophobin/methylcellulose
  • Polydimethylsiloxane surface modification method based on hydrophobin/methylcellulose

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Soak the microchannel of the polydimethylsiloxane chip with 1mol / L NaOH aqueous solution for 5 minutes for activation treatment, then wash it with deionized water until neutral, and then wash it with 20mmol / L PBS buffer solution with a pH value of 7.4 Rinse to obtain the polydimethylsiloxane microfluidic chip after NaOH activation treatment. Add hydrophobin SC3 and methylcellulose into 20mmol / L PBS buffer with a pH value of 7.4, add 1.0mg hydrophobin SC3 and 0.5g methylcellulose to each liter of PBS buffer, and prepare a surface modifier. Use a syringe to inject a surface modifier into the microchannel of the polydimethylsiloxane microfluidic chip after NaOH activation treatment, incubate at room temperature for 15 minutes, and heat at 65°C for 30 minutes to obtain surface-modified polydimethylsiloxane oxane.

Embodiment 2

[0026] In this example, the method for activating the polydimethylsiloxane microfluidic chip with NaOH is the same as that in Example 1. Add hydrophobin SC3 and methylcellulose to 10mmol / L borax buffer with a pH value of 9.5, add 1.0mg hydrophobin SC3 and 0.5g methylcellulose to each liter of borax buffer to prepare a surface modifier, Use a syringe to inject a surface modifier into the microchannel of the polydimethylsiloxane microfluidic chip after NaOH activation treatment, incubate at room temperature for 15 minutes, and heat at 65°C for 30 minutes to obtain surface-modified polydimethylsiloxane oxane.

Embodiment 3

[0028] In this example, the method for activating the polydimethylsiloxane microfluidic chip with NaOH is the same as that in Example 1. Add hydrophobin SC3 and methylcellulose to 50mmol / L Tris-HCl buffer with a pH value of 8.0, add 0.5mg hydrophobin SC3 and 0.5g methylcellulose to each liter of borax buffer, and prepare surface modification agent, use a syringe to inject the surface modifier into the microchannel of the polydimethylsiloxane microfluidic chip after NaOH activation treatment, incubate at room temperature for 20 minutes, and heat at 60°C for 30 minutes to obtain surface-modified polydimethylsiloxane base siloxane.

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Abstract

The invention discloses a polydimethylsiloxane surface modification method based on hydrophobin / methylcellulose. The hydrophobin SC3 and the methylcellulose are used as the surface modifier to be physically adsorbed onto the surface of a micropassage of the polydimethylsiloxane to form an amphipathic monomolecular film, then a chip passage is heated, so that the structure conversion of the hydrophobin SC3 can be induced by the methylcellulose, and a stable amphipathic monomolecular film is formed on the surface of the micropassage of the polydimethylsiloxane. By adopting the method, the coating stability of the polydimethylsiloxane can be improved, the surface hydrophily of the passage can be improved, the non-specificity adsorption of the protein on the surface of the micropassage can be effectively prevented, the antipollution capacity of the surface of the polydimethylsiloxane can be improved, and the high-efficiency reproducibility and separation of an analyzed substance can be realized.

Description

technical field [0001] The invention belongs to the technical field of separation of microfluidic chips, and in particular relates to a method for surface modification of polydimethylsiloxane microfluidic chips. Background technique [0002] Microfluidic chip, also known as micro-total analysis system, is a new technology developed in the 1990s that basically integrates sample preparation, reaction, separation and detection on a single microchip. It has been widely used in life sciences, There are many research fields such as biomedicine, environmental science and chemistry. Polydimethylsiloxane (PDMS) is often used as a matrix material for microfluidic chips because of its low price, stable chemical properties, and good biocompatibility. It is unstable and has strong adsorption to various analytical substances such as proteins, which leads to the reduction of the performance of PDMS microfluidic chips, which in turn affects its application range. [0003] Physisorption co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/04C08J7/12C09D189/00C09D101/26C09D5/16
Inventor 党福全刘超
Owner SHAANXI NORMAL UNIV
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