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Method for modifying polydimethylsiloxane by hydrophobic coating self-assembly by using ion complementary peptide

A dimethylsiloxane, hydrophobic coating technology, applied in coatings, chemical instruments and methods, laboratory containers, etc., can solve the problems of low anti-protein adsorption performance, unstable coatings, etc. Enhanced protein adsorption performance, enhanced coating stability, and inhibition of non-specific adsorption

Inactive Publication Date: 2015-04-08
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the shortcomings of physical adsorption coating method such as unstable coating and low protein adsorption resistance, and provide a method for ion-complementary peptide self-assembled hydrophobic coating modified polydimethylsiloxane , the polydimethylsiloxane microfluidic chip modified by this method has good anti-protein adsorption performance and can significantly inhibit the non-specific adsorption of proteins

Method used

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  • Method for modifying polydimethylsiloxane by hydrophobic coating self-assembly by using ion complementary peptide
  • Method for modifying polydimethylsiloxane by hydrophobic coating self-assembly by using ion complementary peptide
  • Method for modifying polydimethylsiloxane by hydrophobic coating self-assembly by using ion complementary peptide

Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1. Preparation of surface modifier

[0028] The ion-complementary peptide EAR16-II was added to 10mmol / L phosphate buffer with a pH value of 9.5, and 1.0g of ion-complementary peptide EAR16-II was added to each liter of phosphate buffer to prepare a surface modifier.

[0029] 2. Modified polydimethylsiloxane

[0030] The polydimethylsiloxane microfluidic chip was ultrasonically treated with an ultrasonic cleaner in 1.0mol / L NaOH aqueous solution for 15 minutes, then ultrasonically treated with deionized water until neutral, and then washed with 10mmol / L pH value of 9.5 Washing with phosphate buffer solution to obtain the polydimethylsiloxane microfluidic chip after NaOH activation treatment. The surface modifier is injected into the channel of the polydimethylsiloxane microfluidic chip after the NaOH activation treatment to obtain a surface-modified polydimethylsiloxane microfluidic chip.

Embodiment 2

[0032] In step 1 of this example, the ion-complementary peptide EAR16-II and methylcellulose were added to 10 mmol / L phosphate buffer with a pH value of 9.5, and 1.0 g of ion-complementary peptide EAR16 was added to each liter of phosphate buffer -II and 0.5g methylcellulose, formulated as a surface modifier. The other steps were the same as in Implementation 1 to obtain a surface-modified polydimethylsiloxane microfluidic chip.

Embodiment 3

[0034] In step 1 of this example, the ion-complementary peptide EAR16-II and dodecyl-β-D-maltoside were added to 10 mmol / L phosphate buffer with a pH value of 9.5, and each liter of phosphate buffer was added 1.0 g of ion-complementary peptide EAR16-II and 0.5 g of dodecyl-β-D-maltoside were prepared as surface modifiers. The other steps were the same as in Implementation 1 to obtain a surface-modified polydimethylsiloxane microfluidic chip.

[0035] The methods of atomic force microscopy, Fourier transform attenuated total reflection infrared spectroscopy and contact angle measurement were used to analyze the polydimethylsiloxane microfluidic chip and the surface-modified polydimethylsiloxane obtained in Examples 1-3. Oxane microfluidic chip for characterization, the results are as follows Figure 1-5 .

[0036] Depend on Figure 1~4 It can be seen that the surface of the polydimethylsiloxane microfluidic chip is smooth, and the ion-complementary peptide EAR16-II on the po...

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Abstract

The invention discloses a method for modifying polydimethylsiloxane by hydrophobic coating self-assembly by using an ion complementary peptide, which comprises the following steps: by using an ion complementary peptide EAR16-II or ion complementary peptide EAR16-II, methyl cellulose and dodecyl-beta-D-maltoside as a surface modifier, carrying out physical adsorption to perform self-assembly on the polydimethylsiloxane microchannel surface so as to form a stable hydrophobic film; and in the presence of saccharide derivatives, changing the configuration of the EAR16-II to form the more stable beta-folding self-assembled hydrophobic film. The method can effectively inhibit the non-specific adsorption of the protein to the microchannel surface, enhances the surface anti-pollution capacity and biological compatibility of the polydimethylsiloxane, and implements high-efficiency recurrent separation of the analyte.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chip separation, and in particular relates to a method for modifying the surface of a polydimethylsiloxane microfluidic chip to realize the antifouling property of the surface. Background technique [0002] Polydimethylsiloxane (PDMS) is a widely used material for making microfluidic chips. It has the advantages of non-toxicity, good air permeability, good chemical inertness and optical properties, but the surface of PDMS is highly hydrophobic, The electroosmotic flow is unstable, and the adsorption of analytes, especially proteins, is serious. The most effective way to overcome the shortcomings of PDMS is to modify the chip surface. [0003] At present, the commonly used surface modification methods mainly include physical adsorption coating method and chemical bonding coating method. The chemical bonding coating method is a method with cumbersome steps, and PDMS itself is chemically inert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/04B01L3/00C08L83/04
Inventor 党福全于晓玲肖君竹
Owner SHAANXI NORMAL UNIV
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