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Three-dimensional carrier of microarray biochip and preparation method thereof

A biochip and microarray technology, applied in the field of detection, can solve the problems of complex surface activation, high background signal, high price, etc., and achieve the effects of inhibiting non-specific adsorption, improving signal-to-noise ratio, and increasing surface density

Inactive Publication Date: 2013-06-12
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these commercial chips have different defects, such as complex surface activation, high price, poor reproducibility, high background signal, etc., which are difficult to meet the needs of scientific research and practical applications.

Method used

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  • Three-dimensional carrier of microarray biochip and preparation method thereof
  • Three-dimensional carrier of microarray biochip and preparation method thereof
  • Three-dimensional carrier of microarray biochip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation method of the microarray biochip three-dimensional carrier, the specific steps are as follows:

[0035] a. Immerse the clean slide in 5 mM newly prepared potassium permanganate solution for 20 minutes, take it out and rinse it with deionized water; then place the slide in a solution containing 5% (v / v) ethanolamine and 5% (v / v) v) In the 50 mM zinc nitrate solution of ammonia water, grow for 50 minutes in a water bath at 85°C to form zinc oxide nanorods on the surface of the glass slide, take it out and rinse it with deionized water, dry it, and then observe it with scanning electron, the results are as follows figure 2 shown;

[0036] b. Immerse the slide treated in step a in an ethanol solution containing 3% (v / v) 3-epoxypropyloxypropyltrimethoxysilane (APTES) for 2 hours to make the 3-epoxy Propyloxypropyltrimethoxysilane forms silicon-oxygen covalent bonds with zinc oxide nanorods to modify the surface of zinc oxide nanorods, rinses with ethanol, a...

Embodiment 2

[0041] The preparation method of the microarray biochip three-dimensional carrier, the specific steps are as follows:

[0042] a. Immerse the clean slide in 1 mM freshly prepared potassium permanganate solution for 30 minutes, take it out and rinse it with deionized water; then place the slide in a solution containing 1% (v / v) ethanolamine and 1% (v / v) v) Grow in a 10 mM zinc nitrate solution in concentrated ammonia water for 60 minutes in a 65°C water bath to form zinc oxide nanorods on the surface of the glass slide, take it out and rinse it with deionized water and dry it;

[0043] b. Immerse the slide treated in step a in an ethanol solution containing 1% (v / v) 3-epoxypropyloxypropyltrimethoxysilane (APTES) for 3 hours to make the 3-ring Oxypropyloxypropyltrimethoxysilane forms a silicon-oxygen covalent bond with the surface of zinc oxide nanorods to modify the surface of zinc oxide nanorods, rinses with ethanol and anneals in vacuum at a temperature of 110 ° C for 3 hours...

Embodiment 3

[0047] The preparation method of the three-dimensional carrier of the microarray biochip, the specific steps are as follows:

[0048] a. Immerse the clean slide in 10 mM freshly prepared potassium permanganate solution for 15 minutes, take it out and rinse it with deionized water; then place the slide in a solution containing 0.25% (v / v) ethanolamine and 5% (v / v) In 100 mM zinc nitrate solution of concentrated ammonia water, grow in a 95°C water bath for 60 minutes to form zinc oxide nanorods on the surface of the glass slide, rinse with deionized water and dry, then observe with scanning electron, the results are as follows figure 2 shown;

[0049] b. Immerse the slide treated in step a in an ethanol solution containing 5% (v / v) 3-epoxypropyloxypropyltrimethoxysilane (APTES) for 1 hour to make the 3-epoxy Propyloxypropyltrimethoxysilane forms a silicon-oxygen covalent bond with the surface of zinc oxide nanorods to modify the surface of zinc oxide nanorods, rinses with eth...

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Abstract

The invention discloses a three-dimensional carrier of a microarray biochip and a preparation method thereof. The three-dimensional carrier of a microarray biochip is prepared by growing a zinc oxide nanorod on the surface of a substrate material and growing a polymer brush on the surface of the zinc oxide nanorod. The preparation method comprises the following steps of: activating the surface of the substrate material, and growing a zinc oxide nanorod on the surface of the substrate material; performing surface modification on the grown zinc oxide nanorod; connecting the modified surface of the zinc oxide nanorod with an initiator; and finally, growing a polymer brush to obtain a three-dimensional carrier of a microarray biochip. The preparation method is simple, does not need special equipment, has low production cost and good repeatability, and can be used for realizing large-scale production; the prepared three-dimensional carrier has low price and high fixing density for probe molecule, can be used for effectively inhibiting non-specific protein adsorption and improving the sensitivity and the specificity, and can be widely applied to the fields of biology, basic medicine, disease diagnosis, drug screening, food safety, environmental monitoring and the like.

Description

[0001] technical field [0002] The invention belongs to the detection field, relates to a three-dimensional carrier of a microarray biochip, and also relates to a preparation method of the three-dimensional carrier. Background technique [0003] Microarray biochip is to immobilize different biological probe molecules such as DNA, antigen, antibody, aptamer, etc. on a certain carrier in an orderly, high-density, addressable manner, and cooperate with a fluorescent reading system to realize large-scale, A technology for high-throughput detection of biomolecules and the study of biomolecular interactions is of great significance in early screening, diagnosis and treatment of diseases, drug development, and basic life science research. In recent years, it has received extensive research interest from academia and industry . At present, the carrier materials used in microarray biochips are mainly surface-functionalized glass slides. One of the main problems of this type of carr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C40B40/06C40B40/10C08F292/00
Inventor 李长明胡卫华
Owner SOUTHWEST UNIV
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