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Surface modification method for QCM wafer

A surface modification and chip technology, which is applied in the field of biosensors, can solve problems affecting the accuracy of measurement, adverse reactions, changes, etc., and achieve the effects of improving accuracy, good biocompatibility, and high sensitivity

Active Publication Date: 2019-04-02
南通市产品质量监督检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing wafer materials often have microscopic processes such as non-specific attachment, agglomeration, and repulsion of biomass in biological media, resulting in changes in the energy, frequency, and phase of oscillations and interference, which is the so-called Missing Mass ) effect adverse reactions, affecting the accuracy of measurement

Method used

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  • Surface modification method for QCM wafer
  • Surface modification method for QCM wafer
  • Surface modification method for QCM wafer

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Embodiment

[0021] The surface modification method of the present embodiment QCM wafer, this surface modification method comprises the steps:

[0022] Step 1: Prepare 50 mL of ethanol solution containing 1% by mass of ethyl 3-mercaptopropionate, put 12 gold-plated QCM chips into 12-well culture plates, add 3 mL of the above solution to each well, and soak for 1 h Finally, remove the solution, and then use 95% ethanol to clean the surface of the wafer and wait for it to dry naturally;

[0023] Step 2: prepare 20mL polymer A mass percent is 0.5% ethanol solution, wherein the structural formula of polymer A is

[0024]

[0025] Place the QCM wafer processed in step 1 on a glass slide, add the ethanol solution of the polymer dropwise to completely cover the wafer surface, use a mask with a grid width and spacing of 0.1mm to cover the wafer surface, and use a power of 60mW and a wavelength of 250nm ultraviolet light is irradiated for 1 minute to bond the polymer to the surface of the wafer...

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Abstract

The invention relates to a surface modification method for a QCM wafer. The QCM wafer with the gold-plated surface is soaked with an ethanol solution of ethyl 3-mercaptopropionate, ethanol is used forcleaning the surface, and the surface is naturally air-dried; the treated QCM wafer is soaked with a photoactive polymer solution with an active ester group, a strip-shaped or grid-shaped mask is used for covering the surface of the wafer, and a polymer is bonded with the surface of the wafer under the induction of ultraviolet light to form an array structure; absolute ethanol and deionized waterare used for sequentially cleaning the surface of the wafer to remove the unreacted bonded polymer; and the wafer is soaked with a buffer solution containing an antibody, the wafer is cleaned by using the deionized water, and the wafer is stored in the buffer solution for later use. The method has the advantages that a polymer film with a certain pattern structure is prepared on the surface of the QCM wafer through a light-induced grafting technology, and antibody molecules are further grafted to the surface of the film to form an ''antenna'' array capable of capturing specific biomolecules through an antibody-antigen reaction, so that the sensitivity is high.

Description

technical field [0001] The invention belongs to the technical field of biosensors, in particular to a method for modifying the surface of a QCM chip. Background technique [0002] Biosensor is an important tool in the field of food detection. It realizes the analysis and monitoring of target components by combining biometric components and signal conversion components. It has good selectivity, no reagents, easy operation, reusability and Online analysis and other advantages. In recent years, a variety of biosensors based on different principles have been developed and used to detect harmful ingredients in food. Among them, a technology that combines the principle of immunity with Quartz Crystal Microbalance (QCM) has received extensive attention. [0003] QCM utilizes the piezoelectric effect of quartz crystal to convert the change of the surface quality of the quartz crystal electrode into the frequency change of the output electrical signal of the quartz crystal oscillato...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N5/00
CPCG01N5/00G01N33/5302
Inventor 陈刚邵彪李子寅王彦军
Owner 南通市产品质量监督检验所
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