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Vibrio qinghaiensis Q67B and separation, screening and application thereof

A technology for separation and screening of Vibrio qinghai, which is applied to the measurement/inspection of bacteria, biochemical equipment and methods, and microorganisms, and can solve problems such as poor repeatability of test results, unsatisfactory results, and influence on the luminescence stability of marine luminescent bacteria

Inactive Publication Date: 2013-04-17
LIHE TECH (HUNAN) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, some researchers used glucose instead of NaCl to adjust the osmotic pressure, making it isotonic with a NaCl solution with a concentration of 3% by mass, but the effect was not satisfactory, and the repeatability of the test results was poor.
This is because marine luminescent bacteria must have a certain concentration of Na ions in order to maintain good and stable luminescent performance. An environment lacking Na ions is not conducive to the luminescence of marine luminescent bacteria, which will inevitably affect the stability of marine luminescent bacteria. , leading to serious deviations in the detection results

Method used

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  • Vibrio qinghaiensis Q67B and separation, screening and application thereof
  • Vibrio qinghaiensis Q67B and separation, screening and application thereof
  • Vibrio qinghaiensis Q67B and separation, screening and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 The first embodiment of the method for isolating and screening Vibrio Qinghai Q67B

[0066] In the first step, in (1), place at room temperature for 10 hours, in (2), incubate at 20°C for 10 hours, in (3), perform streak separation and culture and repeat three times;

[0067] In the second step, in (1), transfer to five inclined test tubes, in (2), incubate at 20°C for 16 hours, in (3), transfer to five inclined test tubes respectively, in (4), incubate at 20°C 16 hours. In (5), Group I and Group II were cultured at 4°C and 40°C for 16 hours respectively. In (6), Group III and Group IV were cultured at 40°C and 4°C for 16 hours respectively. ( 7) Incubate at 20°C for 16 hours;

[0068] In the third step, in (1), shake culture at 20°C for 16 hours; in (2), shake culture at 20°C for 16 hours; in (3), shake culture at 20°C for 16 hours; in (4), culture at 20°C for 16 hours , (5), incubate at 20°C for 16 hours;

[0069] In the fourth step, in (2), the strain co...

Embodiment 2

[0070] Example 2 The second embodiment of the method for isolating and screening Vibrio Qinghai Q67B

[0071] In the first step, in (1), place at room temperature for 17 hours, in (2), incubate at 20°C for 14 hours, in (3), perform streak separation and culture and repeat three times;

[0072] In the second step, in (1), transfer to eight inclined test tubes, in (2), incubate at 20°C for 17 hours, in (3), transfer to eight inclined test tubes respectively, in (4), incubate at 20°C 17 hours. In (5), Group I and Group II were cultured at 4°C and 40°C for 17 hours respectively. In (6), Group III and Group IV were cultured at 40°C and 4°C for 17 hours respectively. ( 7) Incubate at 20°C for 17 hours;

[0073] In the third step, in (1), shake culture at 20°C for 17 hours; in (2), shake culture at 20°C for 17 hours; in (3), shake culture at 20°C for 17 hours; in (4), culture at 20°C for 17 hours , (5), incubate at 20°C for 17 hours;

[0074] In the fourth step, in (2), the strain...

Embodiment 3

[0075] Example 3 The third embodiment of the method for isolating and screening Vibrio Qinghai Q67B

[0076] In the first step, in (1), place at room temperature for 24 hours; in (2), incubate at 20°C for 18 hours; in (3), perform streak separation and culture five times;

[0077] In the second step, in (1), transfer to ten inclined test tubes, in (2), incubate at 20°C for 18 hours, in (3), transfer to ten inclined test tubes respectively, in (4), incubate at 20°C 18 hours. In (5), Group I and Group II were cultured at 4°C and 40°C for 18 hours respectively. In (6), Groups III and Group IV were cultured at 40°C and 4°C for 18 hours respectively. ( 7) Incubate at 20°C for 18 hours;

[0078] In the third step, in (1), shake culture at 20°C for 18 hours; in (2), shake culture at 20°C for 18 hours; in (3), shake culture at 20°C for 18 hours; in (4), culture at 20°C for 18 hours , (5), incubate at 20°C for 18 hours;

[0079] In the fourth step, in (2), the strain corresponding t...

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Abstract

The invention relates to a vibrio qinghaiensis Q67B (CCTCC (China Center for Type Culture Collection) NO: M2010104) and separation, screening and an application of vibrio qinghaiensis Q67B, and belongs to the technical field of the separation, the screening and the application of luminous bacteria of a microbiology. Vibrio qinghaiensis Q67B is a freshwater luminous bacterium by separating and purifying luminous bacteria collected on the body surface of Qinghai kaluga produced from Qinghai Lake, Qinghai Province, and screening through temperature adaptability, then screening through pH value adaptability, and finally screening through poison reaction sensitivity. Vibrio qinghaiensis Q67B is constantly luminous in a clean water body, when the bacterium contacts poison, the luminous intensity is obviously reduced, so the toxicity and concentration of the poison can be judged. When vibrio qinghaiensis Q67B is used for poison detection of surface water such as inland lakes and rivers, groundwater and sewage effluent, the poison detection can be carried out at 10-37 DEG C without adjusting an osmotic pressure and a pH value of water sample, and the poison of the water sample can be distinguished in 10-15min, so that vibrio qinghaiensis Q67B has the advantages of speediness, convenience and sensitivity. Ocean luminous bacteria do not have the above advantages.

Description

technical field [0001] The invention relates to Qinghai Vibrio Q67B and its isolation, screening and application, and belongs to the technical field of microbiological luminescent bacteria isolation, screening and application. Qinghai Vibrio Q67B is a freshwater luminescent bacterium. Freshwater luminescent bacteria were collected from the surface of the Qinghai sturgeon produced in Qinghai Lake in Qinghai Province. After isolation, purification and screening, a new freshwater luminescent bacteria strain was obtained. 16SrDNA sequence analysis showed that the bacterium belongs to the genus Vibrio and named It is "Qinghai Vibrio Q67B". It was sent to Wuhan China Center for Type Culture Collection (CCTCC) for preservation on January 28, 2010, and the preservation number is CCTCC NO: M2010104. Background technique [0002] Luminescent bacteria are a large class of bacteria that can fluoresce by themselves, and their luminescence is constant when the conditions are right. If ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/02C12R1/63
Inventor 朱文杰
Owner LIHE TECH (HUNAN) CO LTD
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