Paper chip aptamer sensor and preparation method thereof

Abstract

The invention relates to a paper chip aptamer sensor and a preparation method thereof. The paper chip aptamer sensor comprises four layers of filter paper, microfluidic channels (1), filter membranes(2), reaction zones (3), counter electrodes (4), reference electrodes (5) and working electrode (6); the four layers of filter paper are a first layer of filter paper, a second layer of filter paper,a third layer of filter paper and a fourth a layer of filter paper which are distributed sequentially from top to bottom; the microfluidic channels (1) are disposed on the first layer of filter paperand the third layer of filter paper; the filter membranes (2) are disposed on the first layer of filter paper, the second layer of filter paper, and the third layer of filter paper, and are communicated with the microfluidic channels (1); the reaction zones (3) are arranged on the third layer of filter paper and are communicated with the microfluidic channels (1) on the third layer of filter paper; the counter electrodes (4) and the reference electrodes (5) are disposed on the second layer of filter paper and are located above the reaction zones (3); the working electrodes (6) are disposed onthe fourth layer of filter paper and are located below the reaction zones (3); the surfaces of the working electrodes (6) are modified with a nanocomposite and are fixedly provided with aptamers.

Description

technical field [0001] The present disclosure relates to a paper chip aptamer sensor and a preparation method thereof. Background technique [0002] Nucleic acid aptamer is a short oligonucleotide sequence obtained through in vitro screening, which can bind to target molecules with very high affinity. Compared with antibodies, aptamers are cheap; they have no immune properties and can be stored at room temperature; they have a wide range of receptors, and can be used for small molecules, proteins, antibiotics, peptides, cells, pathogenic bacteria, viruses, etc. in addition to antigens detection. Therefore, aptasensors prepared based on the principle of aptamer-antigen specific binding have the advantages of low cost, high stability, high detection sensitivity and selectivity. [0003] Microfluidic paper chip is an emerging microfluidic analysis technology platform established on paper. It has the advantages of low cost, good biocompatibility, mature preparation technology ...

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Problems solved by technology

[0004] The overexpression of growth factor receptor family (EGFR, HER2, HER3, HER4) and the detection of EGFR exon 19 deletion mutation and exon 21 L858R mutation are of great significance to clinical treatment. Challenges such as rapid, low-cost and portable detection

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