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Plant source antiviral agent containing phyllanthi fructus and preparation method thereof

An antiviral agent, the technology of emblica, which is applied in botany equipment and methods, plant growth regulators, disinfectants, etc., achieves the effects of simple preparation method, significant activity, and good environmental compatibility

Active Publication Date: 2014-05-07
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on the chemical constituents of the root bark of Amla emblica, and it has been reported that diterpenoids, myrrhane-type sesquiterpenoids and glycosides were isolated from the root bark of Amla emblica
However, the research on the anti-plant virus effect of Amla emblica has not been reported so far, and its application in agriculture still needs to be further developed.

Method used

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  • Plant source antiviral agent containing phyllanthi fructus and preparation method thereof
  • Plant source antiviral agent containing phyllanthi fructus and preparation method thereof
  • Plant source antiviral agent containing phyllanthi fructus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the preparation of 30% emblica microemulsion

[0039] Weigh 30kg of emblica root bark concentrate, dissolve it in 20kg of ethyl acetate, add 15kg of calcium dodecylbenzenesulfonate, 5kg of styrene acid polyoxyethylene ether, mix under high-speed stirring, and heat to about 40°C Add 25kg of deionized water dropwise under high-speed stirring, control the rate of addition of water, and keep the temperature at about 40°C; after adding the water dropwise, heat up to 50°C and stir for 1 hour to obtain 100kg of 30% emblica microemulsion. The stability and appearance of the preparation meet the requirements of commercial preparations.

Embodiment 2

[0040] Embodiment 2: the preparation of 10% emblica microemulsion

[0041] Weigh 10kg of emblica root bark concentrate, dissolve it in 30kg of ethyl acetate, then add 15kg of calcium dodecylbenzenesulfonate and 5kg of styrene acid polyoxyethylene ether, mix under high-speed stirring, and heat to about 40°C Add 40 kg of deionized water dropwise under high-speed stirring, control the rate of addition of water, and keep the temperature at about 40 ° C; after the addition of water, the temperature is raised to 50 ° C, stirred for 1 hour, and 100 kg of 10% emblica microemulsion is obtained. The stability and appearance of the preparation meet the requirements of commercial preparations.

Embodiment 3

[0042] Embodiment 3: the preparation of 30% emblica root bark soluble agent

[0043]Weigh 30kg of emblica root bark concentrate, dissolve it in 30kg of ethyl acetate, then add 5kg of alkylphenol polyoxyethylene ether, 1kg of alkyl polyglycoside A, 5kg of ethylene glycol, and make up to 100kg with ethanol, and mix well , stirring at a stirring speed of 800 to 1000 rpm for 10 to 30 minutes to prepare 100 kg of 30% emblica soluble agent. The stability and appearance of the preparation meet the requirements of commercial preparations.

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Abstract

The invention discloses a plant source antiviral agent which takes a phyllanthi fructus L. extractive as an effective component and a preparation method thereof. The preparation method comprises the following steps: crushing phyllanthi fructus L. and carrying out a certain processing process to prepare a phyllanthi fructus L. antiviral soluble liquid agent, an aqueous agent and micro-emulsion. The antiviral agent can be used for preventing and treating tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), potato virus X (PVX), potato virus Y (PVY) and the like on crops including tobaccos, chilies, tomatoes, pumpkins, potatoes and the like. The preparation contains 10%-30% of the phyllanthi fructus L. extractive and a residual amount of an auxiliary agent. The plant antiviral agent not only has a good antiviral effect, but also is safe to environments, human beings, livestock and natural enemies of pests, and other beneficial organisms. The plant antiviral agent is simple in preparation process and low in cost, and is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the field of plants and applications thereof, and in particular relates to an anti-plant virus disease preparation of Amla emblica and a preparation method thereof. Background technique [0002] Plant virus is an intracellular parasitic pathogen composed of nucleic acid and protein with infectious activity. According to the eighth report of the International Committee on Taxonomy of Viruses (ICTV), there are currently 1122 plant virus species found in the world. Phytopathogens caused by plant viruses are known as "plant cancers", and their damage to crops is second only to fungal pathogens. Almost every crop is harmed by 2 to 3 viruses. The annual loss caused by plant viruses in the world is about 40 billion U.S. dollars, and the damage caused by tobacco mosaic virus (TMV) alone exceeds 100 million U.S. dollars. [0003] Since plant viruses are absolutely parasitic to plant cells, the material and energy sites required for vir...

Claims

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Application Information

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IPC IPC(8): A01N65/18A01P1/00
Inventor 闫合韩立荣尹红园周勇吴华冯俊涛张兴
Owner NORTHWEST A & F UNIV
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