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Method for removing potato virus by virazole

A potato virus and ribavirin technology, applied in the biological field, can solve the problems of plant poisoning, unscientific, and influence on plant growth and development, and achieve the effect of convenient operation and high virus removal rate

Inactive Publication Date: 2012-07-25
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the addition of these virus inhibitors has also produced toxicity to the plant itself, and even at very low concentrations, the growth and development of the plant will be severely affected
Therefore, it is unscientific to use virus inhibitors to prevent virus infection in normal plants in advance.

Method used

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  • Method for removing potato virus by virazole
  • Method for removing potato virus by virazole
  • Method for removing potato virus by virazole

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 ribavirin removes PVX

[0029] Inoculate PVX-infected potato plantlets into MS medium containing ribavirin at a certain concentration (0mg / L, 75mg / L, 100mg / L, 150mg / L, 200mg / L), under light intensity of 50-80mol / L m 2 / s, the light time is 16h / D, and the cultivation temperature is 20°C for cultivation in a cultivation room. One cultivation cycle is 45 days. After one cycle, the top of the plant is inoculated into the next same concentration medium for the next cycle of cultivation. The rest of the plants were tested by ELISA and measured for plant height and fresh weight (results are shown in Table 1). The results show that the virus source plants infected with PVX alone can be treated for 90 days at a concentration of 75mg / L-150mg / L, and the virus removal rate can reach 100% (the results are shown in Table 6).

[0030] Table 1 Plant height and fresh weight of different concentrations of ribavirin-treated potato test-tube plantlets infected with PVX

[0...

Embodiment 2

[0033] Embodiment 2 ribavirin removes PVM

[0034] Inoculate the potato test-tube plantlets compound-infected with MSY into the MS medium containing ribavirin at a certain concentration (0mg / L, 75mg / L, 100mg / L, 150mg / L, 200mg / L), at a light intensity of 50- 80mol / m 2 / s, the light time is 16h / D, and the cultivation temperature is 20°C for cultivation in a cultivation room. One cultivation cycle is 45 days. After one cycle, the top of the plant is inoculated into the next same concentration medium for the next cycle of cultivation. The rest of the plants were tested by ELISA and measured for plant height and fresh weight (results are shown in Table 2). For the potato test-tube plantlets of compound infection MSY, the removal rate of each concentration to PVM was between 50%-100% when processing for 45 days, and the removal rate to PVS and PVY was 0%; The removal rate of PVS is between 50%-100%, the removal rate of PVS is between 40%-67%, and the removal rate of PVY is 0%; Th...

Embodiment 3

[0038] Embodiment 3 ribavirin removes PVS

[0039] Inoculate the potato plantlets infected with PVS into the MS medium containing ribavirin at a certain concentration (0mg / L, 75mg / L, 100mg / L, 150mg / L, 200mg / L), under the light intensity of 50-80mol / L m 2 / s, the light time is 16h / D, and the cultivation temperature is 20°C for cultivation in a cultivation room. One cultivation cycle is 45 days. After one cycle, the top of the plant is inoculated into the next same concentration medium for the next cycle of cultivation. The rest of the plants were tested by ELISA and measured for plant height and fresh weight (results are shown in Table 3). The results showed that when the infection was treated alone for 90 days, the removal rate could reach more than 75%; when the treatment was 135 days, all concentrations could completely remove PVS (results are shown in Table 6).

[0040] Table 3 Plant height and fresh weight of different concentrations of ribavirin-treated potato test-tube p...

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Abstract

The invention discloses relates to a method for removing plant viruses by virus inhibitor, in particular to a method for removing potato virus by virazole. The method comprising the step of inoculating potato seedlings infected with the virus on the culture medium containing virazole to cultivate for 45-135 days, wherein the concentration of the virazole in the culture medium is 75-150mg / L. The method for removing potato virus by virazole is simple and easy, is convenient to operate, has high removal efficiency for normally potato viruses, can be used during the production, is convenient to treat material in large volumes and is an efficient potato virus removal technology.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for removing plant viruses by using virus inhibitors, in particular to a method for removing several common potato viruses by using ribavirin. Background technique [0002] Potato cultivation is widely distributed and is the fourth largest cultivated crop in the world. China is the country with the largest potato cultivation area. Potatoes occupy an important position in my country's industrial and agricultural production, and the development of my country's potato planting industry has important strategic significance. However, as a vegetatively propagated potato, potatoes are susceptible to various viruses. Through research, it has been found that there are more than 30 kinds of viruses that harm the growth and development of potatoes, among which the most common and serious ones in my country are: Potato Leafroll Virus (PLRV), Potato Virus Y (PVY), Potato Virus X (PVX), Po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 宋波涛杨立杰柳俊
Owner HUAZHONG AGRI UNIV
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