A kind of betel leaf ether extract and its application as fruit and vegetable fresh-keeping agent
A technology of fruit and vegetable preservatives and extracts, applied in the field of betel leaf ether extract and its application as fruit and vegetable preservatives
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Embodiment 1
[0019] The preparation of embodiment 1 betel leaf ethyl ether extract A
[0020] (1) Take 200g of fresh Piper betel leaves, add 1600mL of anhydrous diethyl ether, and extract with Soxhlet extraction for 24 hours under the condition of a constant temperature water bath at 40°C, concentrate under reduced pressure and recover diethyl ether to obtain a crude extract;
[0021] (2) The medicinal extract obtained in step (1) is adsorbed by activated carbon column, eluted with 60-70% ethanol, collects the eluent of 3 times of column volume, concentrates under reduced pressure and collects, and further passes through AB8 type macroporous resin Adsorbed on the column, eluted with 50-60% ethanol, collected 3 times the column volume of the eluate, concentrated under reduced pressure, and freeze-dried to obtain the ether extract A of betel leaf (2.6g).
Embodiment 2
[0022] The preparation of embodiment 2 Piper betel ether extract B
[0023] (1) Get 500g of fresh betel leaves, add 7.5L of anhydrous diethyl ether, under the condition of a constant temperature water bath of 40°C, extract with Soxhlet extraction for 48 hours, concentrate under reduced pressure and recover diethyl ether to obtain a crude extract;
[0024] (2) The medicinal extract obtained in step (1) is adsorbed by activated carbon column, eluted with 60-70% ethanol, and the eluent of 5 times the amount of column volume is collected, concentrated under reduced pressure and collected, and further passed through D101 type macroporous resin Adsorbed on the column, eluted with 50-60% ethanol, collected 5 times the column volume of the eluate, concentrated under reduced pressure, and freeze-dried to obtain the ether extract B (7.9 g) of Piper betel leaves.
Embodiment 3
[0025] The preparation of embodiment 3 Piper betel ethanol extract C
[0026] (1) Get 200g of fresh Piper betel leaves, add 1600mL of ethanol, and extract with Soxhlet extraction for 24 hours under the condition of a constant temperature water bath of 80°C, concentrate under reduced pressure and recover ethanol to obtain a crude extract;
[0027] (2) The medicinal extract obtained in step (1) is adsorbed by activated carbon column, eluted with 60-70% ethanol, and the eluent of 3 times the amount of column volume is collected, concentrated under reduced pressure and collected, and further passed through D101 type macroporous resin Adsorbed on the column, eluted with 50-60% ethanol, collected 3 times the column volume of the eluate, concentrated under reduced pressure, and freeze-dried to obtain the ethanol extract C of betel leaf (3.1 g).
[0028] According to the literature: "Research on the Antioxidant and Antibacterial Activity of Betel Leaf Extract", Liang Hui, et al., 2011...
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