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221 results about "Virus removal" patented technology

Virus-removing bag and virus-removing method using the same

A virus removal bag for removing viruses from a virus-containing suspension, comprising a pouchy casing (a) having at least one inlet and at least one outlet, and a separation membrane (b) which is securely held in the pouchy casing (a) and which partitions the internal space of the pouchy casing (a) into a first compartment (c) communicating with the inlet and a second compartment (d) communicating with the outlet, wherein at least a part of the separation membrane (b) is made of a virus removal membrane.
Owner:ASAHI KASEI KK

In Vivo Immunomagnetic Hyperthermia Platform for Any Cell or Virus Having a Target Surface Receptor

The invention is a nano-entity conjugate for use in an in vivo immunomagnetic hyperthermia system for the detection and treatment of any cell or virus having a target surface receptor which encompasses a technology platform that can be used for both real-time monitoring of any cell or virus having a target surface receptor and as a delivery platform for certain types of treatment that are conducive for in vivo applications. The system allows of cell or virus enumeration; cell or virus capture; and cell or virus removal from the patient's circulatory system in-vivo using immunomagnetic hyperthermia. The application of immunomagnetic hyperthermia may actually diminish and eventually stop the progression of cancer and other blood borne or blood affected diseases.
Owner:IVDIAGNOSTICS

Virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19' and culture material

InactiveCN101611697AImprove immunitySolve the serious sweet potato virus diseaseCultivating equipmentsPlant tissue cultureDiseaseGermplasm
The invention is a virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19', comprising the following steps: selecting plants which are robust without diseases and insect pests and have characteristics of the variety from growing plants; clipping stem with stem tip; removing visible leaves; sterilizing; peeling the stem tip with 1-2 leaf primodia via a dissecting needle; quickly inoculating into a differentiation and induction culture medium; trimming the sterilized seedling into stem segments with 1-2 internode(s) and switching into an enrichment medium after virus detection; selecting sterilized seedling in vigorous growth to cut into small segments with 1-2 internode(s) and switch into a rooting medium; carrying out domestication on seedling after rooting. The invention has stem tip in virus removal and stem segment in rapid propagation, solves the problems of severe virus disease for sweet potato, poor quality of new cultured breed, germplasm degradation, low yield and the like propagated by using sweet potato block and stem. The invention can artificially control the growing conditions, can not be influenced by nature, and has the advantages of small amount of material use, economical culturing materials, short growth cycle, large propagation coefficient and convenient management and is favor of industrial production.
Owner:周玉玲

Virus removal membrane

This virus removal membrane (10) for removing a virus from a protein-containing solution comprises cellulose, and is provided with: a primary-side surface (1) to which the protein-containing solution is supplied; and a secondary-side surface (2) from which a permeate that has permeated through the virus removal membrane (10) is discharged. When a solution including colloidal gold having a diameter of 20 nm is supplied to the virus removal membrane from the primary side, the colloidal gold is captured by the virus removal membrane (10), and the luminance in a cross section of the virus removal membrane (10) is measured, the value obtained by dividing, by the average of the areas of luminance displacement in the spectrum, the standard deviation of said areas, is in the range of 0.01-1.5 inclusive. Furthermore, in the cross section of the virus removal membrane (10), the thicknesses of regions where colloidal gold having a diameter in the range of 20-30 nm inclusive is captured are in the range of 10.0-30.0 Mum inclusive in a wet state.
Owner:ASAHI KASEI MEDICAL CO LTD

Method for removing PVS viruses of potato test-tube plantlets

The invention provides a method for removing PVS viruses of potato test-tube plantlets. The method comprises the steps of stripping the stem tips of the pretreated test-tube plantlets and then putting the test-tube plantlets into a stem tip culture medium with the stem tip facing upwards and the section facing downwards for culture, next, culturing the stem tips for 40-50 days under the conditions of a temperature within the range of 22+/-2 DEG C, the illumination time of 12h-15h/d and the illumination intensity of 2000-3000lx, in the period, transferring the stem tips above 0.5cm long to an MS culture medium, and continuously culturing the undifferentiated stem tip callus or small plantlets less than 0.5cm long, and finally, detecting the stem tip seedlings by use of enzyme linked immunosorbent assay, and remaining the satisfied stem tip seedlings, thereby obtaining the potato seedlings without PVS through the whole period of 120-180 days. The method is high in PVS virus removal rate, suitable for batch operations of removing the PVS viruses of the potato variety resources, and capable of obtaining the satisfied seedlings at a time and avoiding repeated labor; besides, the method is high in virus removal rate and stable in virus removal effect, and the detection cost can be reduced.
Owner:云南省农业科学院经济作物研究所

New process for the industrial-scale purification of gamma globulins from human plasma for industrial applications

The invention relates to a novel, industrial-scale process for the purification of gamma-immunoglobulins (IgG) starting from plasma or fractions thereof. The method involves two chromatographic steps, i.e. a cation exchange capture chromatography, and then a polishing anion exchange chromatography, ensuring a highly purified end product, which contains no aggregates, and high yields. The process also involves a virus inactivation step by means of a solvent / detergent treatment to inactivate the viruses with a lipid envelope, and a virus removal step by nanofiltering to ensure the removal of the non-enveloped viruses.
Owner:KEDRION

Preparation method of monosialotetrahexosyl ganglioside sodium with high purity

The invention relates to a preparation method of monosialotetrahexosyl ganglioside sodium with high purity. The method comprises the following steps: extracting and purifying fresh pig brains taken as raw materials, wherein the extracting step comprises the steps of preparation of acetone powder, extraction of total lipids of tissues, folch distribution, virus removal and acid-hydrolysis virus inactivation; the purifying step comprises the steps of desalination, silica gel chromatography, reversed phase chromatography, concentration, acetone recrystallization and freeze-drying. The method has the beneficial effects that the adverse effect occurrence rate of an injection prepared from the monosialotetrahexosyl ganglioside sodium with the high purity is decreased by 47% according to the tracking statistics of 100 users after the virus removal and the acid-hydrolysis virus inactivation are carried out on the injection under a specific pH condition.
Owner:HUNAN SAILONG PHARMA

Method for preparing human thrombin from cold-removing glue plasma

The invention discloses a method for preparing human thrombin from cold-removing glue plasma. Firstly, anion resins are used to absorb prothrombin in the cold-removing glue plasma, then elution and filtering are conducted, and a prothrombin solution is obtained; secondly, the prothrombin solution is activated through a CaCL2 solution, and a thrombin solution is obtained; thirdly, S/D viral inactivation is conducted on the thrombin solution; fourthly, chromatography is conducted through cation columns, and a purified thrombin solution is obtained; fifthly, ultrafiltration, dialysis and concentrate are conducted on the thrombin solution, and the thrombin solution with the titer meeting the product specification requirement is obtained; sixthly, a filter element of 20 nm in size is used to conduct virus removal filtering; seventhly, sterilization filtering is conducted through a filter element of 0.22 microns in size, and then subpackage is conducted according to the required specifications; eighthly, freeze-drying is conducted, and dry heat viral inactivation is conducted on freeze-drying powder, and a human thrombin product is obtained. According to the method, the thrombin is purified through the one-step cation columns, operation is simple, thrombin specific activity is high, and safety of clinic use of the product is guaranteed through a three-step virus elimination mode.
Owner:上海洲跃生物科技有限公司

Outdoor transplanting and temporary planting method of stock seedlings of healthy sugarcane seedlings

InactiveCN102577785AImprove tillering rateStrong tillering rateHorticultureAxillary budSucrose
The invention relates to an outdoor transplanting and temporary planting method of stock seedlings of healthy sugarcane seedlings, comprising the following processes: outdoor land preparation and bed preparation; transplantation matrix preparation; sparse planting and transplanting of the stock seedlings; short-term heat preservation and moisture preservation; management of water, fertilizers andpesticides; and outplanting. In the invention, the production technology system of the biochemical virus removal of healthy sugarcane seedlings is established, the technical problems of virus removaland rapid propagation of sugarcane axillary buds are effectively solved; the seedling-growing cost is low; the seedling-growing time is short; the seedling-growing survival rate is high; the obtainedbiochemical virus-free healthy sugarcane seedlings have the characteristics of strong tillering rate, high stem forming rate, rapid growth speed, high ratooning rate and the like; the yield per unit area is improved by more than 30%; the sucrose content is improved by 0.5-1.0%; and the using amount of seedlings is saved by more than 60%. The outdoor transplanting and temporary planting method of the invention has great significance for solving the problems of serious degeneration of the sugarcane varieties and low breeding speed of the certified seedlings, plays an active role of promoting the popularization and application of healthy sugarcane seedlings and has important economic and social benefits.
Owner:INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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