The invention provides an oriental lily seed ball virus removing method, which specifically comprises the following steps of: A) stripping of explant growth points, B) heat treatment virus removal, C) cleaning and sterilization of explants, D) acquisition, induction and culture of growth points, E) virus-free culture and F) virus detection. The oriental lily seed ball virus removing method has the advantages that the defects existing in the prior art are overcome, the defects caused by a reason that a single method is used are overcome by comprehensively using a heat treatment virus removing method, a shoot tip culture virus removing method and a callus virus removing method, the probability of virus cross infection can be effectively reduced, the virus removal rate is high, the survival rate is high and the culture period of seed balls can be effectively shortened.