A strawberry stem tip rapid breeding method by tissue culture and virus removal
A stem tip and strawberry technology is applied in the field of rapid propagation of "Jiuxiang" strawberry stem tip tissue culture and detoxification, which can solve the problems affecting the excellent characteristics of new strawberry varieties, the long cycle of stem propagation and seedling breeding, and restricting the demonstration of "Jiuxiang" strawberry. problems such as application and variety promotion, to achieve the effect of saving seedling land, large application value, and increasing the rate of large fruit
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Embodiment 1
[0025] Example 1 Explant disinfection and shoot tip growth point stripping
[0026] 1. See figure 1 , Select a strong and disease-free "Jiuxiang" strawberry mother plant with a terminal bud of about 1.5 cm in size on the stolon, dip it in a small amount of washing liquid and gently scrub it with your hands for 2 to 3 times, rinse it with running water for 90 to 120 minutes, and then put it on the ultra-clean workbench. Rinse with 75% alcohol for 10 seconds, wash with sterile water for 3 times, sterilize with 0.5% sodium hypochlorite for 7 minutes, rinse with sterile water for 6 to 7 times, and finally blot the surface moisture with sterile filter paper.
[0027] 2. Peel off the leaves of the outer layer of the terminal bud layer by layer under sterile conditions and under a dissecting microscope, and cut off the growth point with a leaf primordia (about 0.2-0.3 mm in length) with a scalpel (see figure 2 ), immediately inoculated on the differentiation medium (recipe: MS+6-BA...
Embodiment 2
[0028] Rooting, acclimatization and transplanting of the multiplication culture of embodiment 2 clustering buds and tissue culture seedlings
[0029] 1. After four weeks, clustered buds grow from the tip of the stem. When the buds grow to 2 cm in size, cut off the buds one by one and divide them into individual plants. Put 5 to 6 buds in each bottle, and then transfer them to the proliferation medium (formula: MS+6 -BA0.2mg / L+IBA0.02mg / L+white sugar 30g / L+agar 8g / L, pH=5.7~5.8) to continue to proliferate, culture temperature 24±1℃, 12 hours / day light nourish. The multiplication coefficient reaches 3, and the statistical results are shown in Table 1 and Table 2 below. One generation is multiplied every 28 days, and a large number of non-toxic seedlings are obtained after multiplication of 6 to 7 generations. See the multiplication results for image 3 .
[0030]2. Cut the strawberry sprouts cultured through subculture from the bud cluster, and transfer them to the rooting med...
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