A kind of in vitro rapid propagation method of Cymbidium cymbidium

Abstract

The invention provides a method for in vitro intermediate propagation of cymbidium grandiflorium. The cymbidium grandiflorium is propagated through a method of plant tissue cultivation, the influenceof external conditions can be avoided, production can be conducted in all seasons, the occupied area of seedlings can be saved, and the costs of enterprises are lowered. According to the method for invitro intermediate propagation of the cymbidium grandiflorium, at different stages, through control of a series of artificial conditions such as provision of different culture media, all good characters of a parent body are preserved, and the inherited characters are stable; and through induction differentiation, adventitious buds are obtained efficiently, test-tube plantlets formed by rooting are treated through acclimatization, the growth cycle can be shortened, the survival rate can be effectively increased, and the method for in vitro intermediate propagation of the cymbidium grandiflorium is suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of artificial propagation and cultivation methods of plants, in particular to a method for in vitro rapid propagation of Cymbidium cymbidium. Background technique [0002] With the continuous improvement of people's living standards, flowers have entered the daily life of ordinary people, and the consumption demand is also increasing. Since ancient times, orchids have been the embodiment of elegance and otherworldliness, and have high ornamental and economic value. In the international market, both cut flower varieties and potted flowers occupy an important position and are very popular among consumers. [0003] Cymbidium, with long green leaves, bold and magnificent flowers, is a world-renowned "Orchid Nova". Its plants and flowers are divided into large and medium-sized, and the flowers are huge, yellow, white, green, red, pink As well as multi-color and other colors, the colors are bright and colorful, ...

AI Technical Summary

Problems solved by technology

[0004] The traditional reproduction of orchids is branch reproduction. However, a robust orchid can only reproduce a few buds a year, the reproduction coefficient is extremely low, and the multiplication multiple is only 1-3 times. Many rare varieties cannot be reproduced in large quantities. It is far from being able to meet the demand of the market, and the price has remained high for a long time, and each leaf bud is as high as tens of thousands of yuan

Many varieties cannot or seldom produce seeds under cultivation conditions. If they reproduce sexually, because the seeds of orchids are small and the embryos are not fully developed, they cannot increase the nutritious endosperm and other tissues, and it is not easy to germinate. It is possible to germinate only when specific symbiotic fungi provide nutrition; many rare varieties are actually some natural mutants. If seeds are produced through the process of sexual reproduction, and then the offspring are reproduced by seeds, then most of the individuals of this variety will lose The unique ornamental value of the original variety, in addition, the long-term asexual reproduction has caused an increasing number of plants with viruses, which has seriously reduced the variety of orchids

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