121 results about "Viral contamination" patented technology

A method for treating biomaterial is provided in which a biological tissue, typically after being cross-linked, is contacted with an anticalcification treatment solution under condition effective to render the biomaterial resistant to in vivo calcification upon implantation in a host animal. The anticalcification treatment solutions comprise higher alcohol solutions, a polyol solutions and / or a polar aprotic organic solvent solutions. Methods of reducing cytotoxicity to host tissue of bioprostheses that comprise fixed animal tissues, and treatments to reduce viral contamination of implantable medical devices are disclosed herein.

The present invention relates, in general, to methods for removing viral contaminants from therapeutic protein solutions to improve safety of therapeutic proteins administered to patients. Particularly contemplated is the removal of small non-enveloped viruses, such as parvovirus, from therapeutic protein solutions.

The invention provides a fully human monoclonal antibody against tetanus toxin. The amino acid sequences of a heavy-chain variable region and a light-chain variable region of the monoclonal antibody are SEQ ID No. 3 and SEQ ID No. 4, respectively; preferably, the amino acid sequences of a heavy chain and a light chain of the monoclonal antibody are SEQ ID No. 1 and SEQ ID No. 2, respectively. The invention further provides a preparation method and application of the fully human monoclonal antibody against tetanus toxin and a derivative thereof. The fully human monoclonal antibody against tetanus toxin provided by the invention can eliminate the biological risks of anaphylactic reaction and virus contamination, has a sufficiently long half life and high titer and in-vivo activity, can be applied to large-scale industrial production.

The invention discloses a clinical-grade serum-free medium for adherent culture of human neural stem cells. The medium disclosed by the invention comprises a basic medium, basic nutrition additives, plant-based human serum albumins, saccharides, lipid, hormones, antioxidants and related substances for promoting metabolism; the basic medium is prepared from commercial DMEM / F12 and a commercial neurobasal medium according to a ratio of 1:1; the basic nutrition additives comprise insulin, holo-transferrin, apo-transferrin, putrescine, progesterone and sodium selenite; the hormones comprise biotin, corticosterone, lipoic acid, Ve and Ve acetic ester; the antioxidants comprise human-derived catalase, human-derived superoxide dismutase, glutathione and Vc; the related substances for promoting metabolism comprise carnitine, T3 and ethanol amine. The medium can improve a cell expansion speed by two to three times, well keeps stem properties of the neural stem cells, keeps the cell differentiation potential of the neural stem cells and eliminates potential animal-origin endotoxin and viral pollution.

The present invention relates to a liquid formulation of a combination of long-acting insulin and insulinotropic peptide, comprising insulin which is a physiologically active peptide, insulinotropic peptide, and albumin-free stabilizer, wherein the stabilizer comprises a buffer, a sugar alcohol, a non-ionic surfactant, and an isotonic agent; and a method for preparing the liquid formulation. The liquid formulation of the present invention does not contain a human serum albumin and potentially toxic factors to the body, and thus it has excellent storage stability for insulin conjugate and insulinotropic peptide conjugate at high concentration, without a risk of viral contamination.

The present invention relates to a plasma product or a serum product with an extremely low risk of viral contamination and a method for producing the same. Before treating plasma or serum to be used as a raw material for producing a plasma product or a serum product using a virus removal membrane, leucocytes contaminating the blood are removed. Thus, a plasma product or a serum product with an extremely low risk of viral contamination can be efficiently produced while preventing clogging. Since clogging scarcely arises, it is possible to carry out efficient filtration without applying an elevated pressure as the filtration proceeds.

A serum-free vegetable protein peptide general cell culture medium relates to a serum-free or low-serum general cell culture medium. The serum-free vegetable protein peptide general cell culture medium solves the problems that the existing serum-free culture medium can not directly culture the cells with adverse growth status, most of cells can be adaptable to the existing serum-free culture medium after the gradual reduction of the serum concentration, and the existing serum-free culture medium is vulnerable to the external factors and easy to cause fatal damage. The serum-free vegetable protein peptide general cell culture medium is prepared by calcium chloride, potassium chloride, magnesium sulfate anhydrous, sodium chloride, anhydrous sodium dihydrogen phosphate, L-glutamine, D-glucose, phenol red, D-calcium pantothenate, choline chloride, folic acid, i-inositol, nicotinamide, pyridoxal hydrochloride, riboflavin, thiamine hydrochloride, soybean protein peptide and distilled water. Cells can be directly cultured on the culture medium of the invention without gradual habituation and culture, thus shortening culture period and avoiding the interference by other factors, reducing the virus pollution which is caused by serum; the cell survival rate reaches more than 94 percent.

The invention relates to a coronavirus pseudovirus packaging system. The coronavirus pseudovirus packaging system comprises a vesicular stomatitis virus VSV vector and an assembly cell, wherein the vesicular stomatitis virus VSV vector is formed by replacing GP genes with Fluc and EGFP double reporter genes, and the assembly cell is used for expressing coronavirus spike protein S. The double reporter genes are selected from luciferase and fluorescent protein, and the luciferase reporter gene is preferably the Fluc gene. According to the packaging system, a one-step packaging method is adopted, so that pseudoviruses which are infected in a single cycle, low in background value and high in titer and have the characteristic of rapid detection compared with a lentivirus-mediated pseudovirus system can be rapidly packaged, and the packaging system can be used for researching coronaviruses such as COVID-19 (SARS-CoV-2), SARS (SARS-CoV) and MERS; and the pseudoviruses can be used for evaluating the efficacy of a disinfectant through the steps of a virus pollution distribution model, scene building and sampling detection, a safe, convenient and effective tool method is provided for evaluating the disinfectant, and the pseudoviruses have wide application value.

The invention discloses a human source anti-tetanus exotoxin antibody (HTAT-Fab) and preparation method, comprising: constructing human immunity phage antibody bank, screening phage positive clone, further getting HTAT-Fab gene with a specific neutralization activity and high affinity. The gene can be expressed in the procaryotic cell such as E.coli, eukaryotic cell such as microzyme, or mammalian cell such as CHO, purifying to get highly purified HTAT-Fab with a strong tissue penetrability, a high affinity. The HTAT-Fab product can not only eliminate the allergic reaction generated by horse serum anti-tatanus antitoxin (TAT) (foreign protein), but also avoid the blood source for producing human tetanus immunoglobulin (HTIG) and the latent virus pollution.

The present invention belongs to the field of food and food processing, in particular to a plant enzyme and a preparation method and a use thereof. The plant enzyme is mainly composed of moringa oleifera leaves, maca, roselle, passiflora coerulea, blueberries and kiwi fruits. The preparation method includes: pulping the plant enzyme raw materials, fermenting in a fermentation tank containing mixed bacteria of bifidobacterium adolescentis, streptococcus thermophilus and lactobacillus and drying. The provided plant enzyme uses food-grade mixed bacteria strains as the fermentation bacteria, fully decomposes nutrients in plant raw materials, and can fully replenish nutrients needed by human body, enhance cellular metabolic function, promote digestion, and enhance human body immunity. At the same time, the food-grade bacteria strains will not appear other bacterial and viral contamination problems in the fermentation process, which realize the security of the plant enzymes. Besides, animal experiments and clinical trials both prove that the plant enzymes have good blood pressure lowering effects.

The present invention relates to a liquid formulation of long-acting insulinotropic peptide conjugate, comprising a pharmaceutically effective amount of long-acting insulinotropic peptide conjugate consisting of a physiologically active peptide, insulinotropic peptide, and an immunoglobulin Fc region; and an albumin-free stabilizer, wherein the stabilizer comprises a buffer, a sugar alcohol, a non-ionic surfactant, and an isotonic agent, and a method for preparing the formulation. For the purpose of preventing microbial contamination, a preservative may be added. The liquid formulation of the present invention is free of human serum albumin and other potentially hazardous factors to body, having no risk of viral contamination, and thus can provide excellent storage stability for insulinotropic peptide conjugates at high concentration.

The invention provides a multi-RT-PCR method for monitoring pollution of six main viruses in a pig farm environment through one system and application. According to the method, primer design is conducted for CSFV, JEV, PRRSV, PCV-2, PRV and PPV, a multi-RT-PCR reaction system for the six viruses is established, air samples are collected through liquid collision, the viruses are subjected to separation concentration through ultrafiltration centrifugation, and the method for detecting the pollution of the main viruses in the pig farm environment is established. The method can be used for monitoring the pollution of the six viruses in air, fodder, drinking water, appliances, excrement and pig groups in the pig house and pig farm environment, and technical support is provided for establishing a main virus pollution early-warning mechanism and a main virus disease preventing, controlling and purifying system in the pig farm environment.

The invention provides a method for cultivating blueberries, which is used for preventing or reducing viral pollution during the process of cultivating blueberries. The method comprises the following steps: performing pretreatment on the blueberries so as to obtain disinfectant root tips; culturing the root tips into regeneration plant sprouts on a culture medium; culturing the sprouts into plantlets on the culture medium; enabling the plantlets to grow into test-tube plantlets on the culture medium; grouping and sampling randomly, and detecting whether samples are infected viruses, especially specific viruses or not. In addition, the invention further provides a method for detecting blueberry viruses synchronously and quickly through multiple RT-PCR, and a mixed prime pair is adopted.

Disclosed is a stable pharmaceutical solution preparation of erythropoietin (EPO), which includes a stabilizing agent not containing a blood-derived protein, thereby maintaining EPO activity for a prolonged period of time without the risk of viral contamination. The stable solution preparation further includes a non-ionic surfactant and a tonicity agent, thereby preventing EPO loss during storage and facilitating administration to the body.

The present invention relates to a liquid formulation of a combination of long-acting insulin and insulinotropic peptide, comprising insulin which is a physiologically active peptide, insulinotropic peptide, and albumin-free stabilizer, wherein the stabilizer comprises a buffer, a sugar alcohol, a non-ionic surfactant, and an isotonic agent; and a method for preparing the liquid formulation. The liquid formulation of the present invention does not contain a human serum albumin and potentially toxic factors to the body, and thus it has excellent storage stability for insulin conjugate and insulinotropic peptide conjugate at high concentration, without a risk of viral contamination.

The present invention provides a method of reducing the activity of prions using vibriolysin or variants thereof. Vibriolysin-containing solutions are used to sanitize prion-contaminated facilities and instruments and decontaminate food products and biological tissues. The present invention provides a method of treating prion-related disease in animals and humans, comprising the administration of a formulation of vibriolysin or a variant thereof together with a pharmaceutically acceptable carrier. Such novel formulations are engineered to track the natural path of the prion from cells where the prions accumulate in the preclinical stage into neuronal cells and the brain at the advanced stage of the disease. The present invention provides methods and formulations that encompasses natural and recombinant vibriolysins and variants thereof with enhanced ability to access prion target cells, and with enzyme activity capable of being regulated by specific conditions, such as pH range or enzymatic cleavage.