Method for proliferating avian influenza viruses in bioreactor with cell carrier

Abstract

The invention discloses a method for replicating and proliferating avian influenza viruses in the passage cells which are absorbed and grow in the microcarrier of a bioreactor. The invention is based on the method which uses cells as carrier to proliferate viruses in the bioreactor. The obtained avian influenza viruses and the avian influenza virus vaccine product have uniform quality and stable toxicity. The automatic control of vaccine production can be realized, the problem of large-scale production and application can be solved, the production matrix of the influenza vaccine can be completely changed, the technology that chick embryo is adopted to culture viruses can be changed, the problems caused by chick embryo such as adventitious agent pollution and viral pollution and the interferences caused by heterologous proteins such as chick embryo can be reduced, the purity, safety and immune effect of vaccine can be increased and the method can play an important role in the prevention of avian influenza.

Description

technical field [0001] The invention belongs to the field of biology, in particular to a process for mass-propagating viruses in a bioreactor by cultivating cells with microcarriers. Background technique [0002] my country has a vast source of wealth, agriculture accounts for 70% of my country's total output value, and aquaculture is an important part of my country's farmers' economic sources. With the occurrence of avian influenza and the large-scale death of poultry, the production of preventive vaccines in my country is far from meeting the needs of the existing aquaculture market, and some vaccines are not effective, which aggravates the plight of the vaccine industry . Therefore, intensifying efforts to develop vaccines is the need of the current epidemic situation and the need of developing our country's national economy. [0003] At present, the virus sources of avian influenza vaccines all come from the culture of chicken embryos. The method is: inoculate the influ...

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Problems solved by technology

[0005] my country's biological products industry uses more primary animal cells in the production of various vaccines, while passage cells are rarely used. So far, there have been no reports and applications of avian influenza viruses using cell carriers for large-scale vaccine production.

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