Non-blood serum plant protein peptide universal cell culture medium
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A plant protein peptide, serum-free technology, applied in animal cells, vertebrate cells, fusion cells, etc., can solve problems such as lethal damage, and achieve the effect of avoiding interference, simplifying the separation and purification process, and maintaining balanced nutrition.
Inactive Publication Date: 2008-08-20
乐能生物工程股份有限公司
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[0004] The purpose of the present invention is to solve the problem that the existing serum-free medium cannot directly cultivate cells with poor growth conditions. Most cells must gradually reduce the serum concentration to adapt to the existing serum-free medium and are easily affected by external factors and easily cause lethal damage. problem, while providing a serum-free vegetable protein peptide universal cell culture medium
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specific Embodiment approach 1
[0012] Specific embodiment one: In this embodiment, 1000mL serum-free plant protein peptide universal cell culture medium is composed of 200mg calcium chloride, 400mg potassium chloride, 97.67mg anhydrous magnesium sulfate, 6800mg sodium chloride, 121.74mg anhydrous sodium dihydrogen phosphate, 292mg L-glutamine, 1000mg D-glucose, 10mg phenol red, 1mg D-calcium pantothenate, 1mg choline chloride, 1mg folic acid, 2mg i-inositol, 1mg niacinamide, 1mg pyridoxal hydrochloride, 0.1mg nucleus Flavin, 1mg thiamine hydrochloride, 5000mg soybean protein peptide and the rest distilled water.
[0013] According to the Chinese patent "Preparation method of soybean protein active peptide" (patent number ZL 00110471.3, publication number CN1273783A), the color and luster of the soybean protein active peptide prepared is slightly milky white or light yellow, has a pleasant smell, and is powdery without agglomeration; in the original patent Soy protein active peptides are only used in food pr...
specific Embodiment approach 2
[0019] Embodiment 2: In this embodiment, HaLe cells (African female cervical cancer cells) are used in serum-free plant protein peptide universal cell culture medium. In this embodiment, the HaLe cells are stored in liquid nitrogen in the laboratory of the Animal Science and Technology College of Bayi Agricultural University.
[0020] Use the serum-free plant protein peptide universal cell culture medium and ordinary MEM cell culture medium (product code: MD600, batch number: 070512) of the specific embodiment respectively to directly cultivate the recovered HaLe cells under the condition of 37 ° C, and use a microscope The cell morphology, growth and proliferation of each group were observed.
[0021] Table 1
[0022]
project
Ordinary MEM cell culture medium
Serum Free Vegetable Protein Peptide
with cell culture medium
Newborn bovine serum volume
10％
0
Seeding cell density
5×10 4
5×10 4
...
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Abstract
A serum-free vegetable protein peptide general cell culture medium relates to a serum-free or low-serum general cell culture medium. The serum-free vegetable protein peptide general cell culture medium solves the problems that the existing serum-free culture medium can not directly culture the cells with adverse growth status, most of cells can be adaptable to the existing serum-free culture medium after the gradual reduction of the serum concentration, and the existing serum-free culture medium is vulnerable to the external factors and easy to cause fatal damage. The serum-free vegetable protein peptide general cell culture medium is prepared by calcium chloride, potassium chloride, magnesium sulfate anhydrous, sodium chloride, anhydrous sodium dihydrogen phosphate, L-glutamine, D-glucose, phenol red, D-calcium pantothenate, choline chloride, folic acid, i-inositol, nicotinamide, pyridoxal hydrochloride, riboflavin, thiamine hydrochloride, soybean protein peptide and distilled water. Cells can be directly cultured on the culture medium of the invention without gradual habituation and culture, thus shortening culture period and avoiding the interference by other factors, reducing the virus pollution which is caused by serum; the cell survival rate reaches more than 94 percent.
Description
technical field [0001] The present invention relates to serum-free or low-serum universal cell culture media. Background technique [0002] Cells are the expression vectors of viruses and proteins, and the quality of cells directly affects the expression level. Only a good medium can screen and domesticate high-quality cells. Ordinary cell culture medium contains calf serum. Because serum is produced in batches, the quality varies greatly among batches and the quality is unstable. Moreover, serum contains some substances that are toxic to cells, which will affect the growth of cells and even cause cell death. At the same time, it is more difficult to separate and purify in industrial production; and it is especially unsuitable for the study of the effect of growth factors on cells, the study of the function of cell secretions, and the preparation of a large number of cell secretions. [0003] Serum-free medium is a synthetic medium that can maintain the growth and reproduct...
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