Method for quickly determining nature of manuka honey

Abstract

The invention relates to the field of detection, in particular to the field of detection of manuka honey, and more particularly relates to a method for quickly determining the nature of manuka honey, which is a molecular fluorescence method and comprises the following steps of: irradiating a sample to be detected by using 270nm as an excitation wavelength, scanning an emission spectrum in a range of 280-550nm, and judging by using fluorescence peaks at 305nm and 360nm and the intensity of the fluorescence peaks. The qualitative detection method disclosed by the invention can be used for rapidly, accurately and qualitatively detecting single-flower manuka honey, multi-flower manuka honey and other adulterated honey. According to the method, expensive instruments such as a high performance liquid chromatograph and the like and complicated separation treatment are not needed, so that the manual honey does not need to be sent to a specific laboratory for detection, and various occasions for qualitative detection of the manual honey in actual work can be met.

Description

technical field [0001] The invention relates to the field of detection, in particular to the field of detection of Manuka honey, and more specifically to a method for rapidly characterizing Manuka honey. Background technique [0002] Manuka honey is a precious honey species unique to New Zealand. It is brewed by bees collecting the nectar of a local shrub plant—Manuka (Leptospermum scoparium). What sets Manuka honey apart from other honeys is its powerful and unique antibacterial activity. Honey generally has antibacterial activity, which is caused by the high permeability of honey itself, strong acidity and generally containing peroxides. The antibacterial activity of manuka honey is not dependent on peroxide, so it is called non-peroxide antibacterial activity (NPA). Medical research has shown that the superior antibacterial activity of Manuka honey has demonstrated its unique medicinal value in preventing wound infection, promoting wound healing, and treating foot ulcer...

AI Technical Summary

Problems solved by technology

[0005] At present, the widely used detection technology is to detect specific characteristic markers, but this method has its disadvantages

First, this method relies on the precise separation of samples, so it usually needs to be equipped with high-performance liquid chromatography and other equipment in the detection, which is not only expensive but also must be sent to a specific laboratory for inspection, which cannot achieve on-site and rapid detection; Finally, the purest Manuka honey comes from the single-flowered Manuka honey, which is called the single-flowered Manuka honey, which is the highest grade Manuka honey, but due to the presence of There are other flower species, so it is very likely to be mixed with other nectars. In the prior art, this honey is called multifloral Manuka honey. Although there are characteristic markers in both, their levels are different, so the existing The detection method relying on a single characteristic marker cannot distinguish between monofloral Manuka honey and polyfloral Manuka honey

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