Detection method for rapidly detecting beta-stimulant and paper-based immunosensor

An immune sensor and stimulant technology, which is applied in the direction of material analysis, instruments, and measuring devices through observation of the impact on chemical indicators, can solve the problems of unsuitable real-time monitoring, time-consuming and labor-intensive detection process, and achieve easy promotion and application. Simple operation and use, good stability

Pending Publication Date: 2021-08-10
安徽省产品质量监督检验研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because its detection requires the assistance of large instruments and the participation of professional and technical personnel, the detection process is time-consuming and laborious, and it is not suitable for real-time monitoring in the food field

Method used

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  • Detection method for rapidly detecting beta-stimulant and paper-based immunosensor
  • Detection method for rapidly detecting beta-stimulant and paper-based immunosensor
  • Detection method for rapidly detecting beta-stimulant and paper-based immunosensor

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preparation example Construction

[0039] S1. Preparation of paper-based microporous plate: design the orifice plate model with drawing software, and the shape and size of the orifice plate model can be changed according to requirements;

[0040] Then, use a color printer to print out the previously designed orifice plate model with hydrophobic wax on cellulose-rich filter paper,

[0041] Place the filter paper with the printed orifice plate model on a magnetic heater, heat at 110-130°C for 2 minutes, so that the hydrophobic wax melts and penetrates into the filter paper, forming a hydrophobic boundary and a circular reaction area, and cools down to room temperature. It is a paper-based microporous plate;

[0042] S2. Preparation of paper-based sensors: Add 3 μL of β-agonist coating source to each circular reaction area of ​​the paper-based microporous plate, then put the paper-based microporous plate in a constant temperature box, and heat temperature, place it for 8-12min, and wait for it to dry, wherein, th...

Embodiment 1

[0057] Embodiment 1: A paper-based immunosensor for rapid detection of clenbuterol hydrochloride, including a paper-based sensor;

[0058] The manufacturing method of the paper-based sensor is as follows:

[0059] S1. Preparation of paper-based microporous plate: use CAD drawing software to design a 96-well plate model with a pore size of 5mm, and then use a Xerox Phaser 8560N model color printer to print out the front design with hydrophobic wax on Whatman No.1 filter paper For the 96-well plate model, place the filter paper printed with the 96-well plate model on a magnetic heater, heat it at 120°C for 2 minutes, and melt the hydrophobic wax into the filter paper to form a hydrophobic boundary and a circular reaction area , cooled to room temperature, that is, a paper-based microporous plate;

[0060] S2. Preparation of paper-based sensors: Add 3 μL of β-agonist coating source to each circular reaction area of ​​the paper-based microporous plate, and then put the paper-base...

Embodiment 2

[0073] Embodiment 2: A paper-based immunosensor for rapid detection of ractopamine, including a paper-based sensor;

[0074] The manufacturing method of the paper-based sensor is as follows:

[0075] S1. Preparation of paper-based microporous plate: use CAD drawing software to design a 96-well plate model with a pore size of 5mm, and then use a Xerox Phaser 8560N model color printer to print out the front design with hydrophobic wax on Whatman No.1 filter paper For the 96-well plate model, place the filter paper printed with the 96-well plate model on a magnetic heater, heat it at 120°C for 2 minutes, and melt the hydrophobic wax into the filter paper to form a hydrophobic boundary and a circular reaction area , cooled to room temperature, that is, a paper-based microporous plate;

[0076] S2. Preparation of paper-based sensors: Add 3 μL of ractopamine-coated raw material to each circular reaction area of ​​the paper-based microporous plate, then put the paper-based microporo...

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Abstract

The invention discloses a detection method for rapidly detecting beta-stimulant and a paper-based immunosensor. The manufacturing method of the paper-based immunosensor comprises the steps of: S1, designing a pore plate model through drawing software, then, printing the pore plate model designed previously on filter paper rich in cellulose through hydrophobic wax by means of a color printer, placing the filter paper printed with the pore plate model on a magnetic heater, heating for 2 minutes, and cooling to room temperature to obtain a paper-based micro-pore plate; and S2, adding beta-stimulant coating antigen into each circular reaction area of the paper-based micro-pore plate, drying the beta-stimulant coating antigen, then adding BSA confining liquid into each circular reaction area of the paper-based micro-pore plate, and drying the BSA confining liquid to obtain the paper-based sensor. According to the invention, the convenient paper-based immunosensor is utilized, so that the beta-stimulant can be quickly and sensitively detected in a colorimetric manner, and powerful technical support is provided for field detection, supervision and the like of the beta-stimulant.

Description

technical field [0001] The invention relates to the technical field of food safety, in particular to a detection method for rapidly detecting β-stimulants and a paper-based immune sensor. Background technique [0002] β-stimulants, also known as clenbuterol, are artificially synthesized drugs. Because they can increase the lean meat rate of livestock and poultry, they are occasionally illegally added to feed. Long-term ingestion of animal tissues containing a large amount of β-receptor agonists by the human body will directly endanger human health. my country has explicitly banned the addition of clenbuterol, ractopamine, salbutamol and other β-agonists to feed or animal drinking water. "Notice on Printing and Distributing the List of Non-edible Substances and Food Additives That May Be Illegally Added in Food (Fourth Batch)" (Rectification Office Letter [2010] No. 50) clearly stipulates that β-stimulant drugs (gram hydrochloride Lentrol, ractopamine, albuterol) cannot be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/01
CPCG01N21/78G01N21/01
Inventor 闫超姚帮本郭佳佳陈伟陈赵然童宁汪龙余
Owner 安徽省产品质量监督检验研究院
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