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Colloidal gold chromatography semi-quantitative determination 2,4-D test paper and preparation method thereof

A semi-quantitative, colloidal gold technology, applied in 2 fields, to achieve the effects of sensitivity and stability, simple reaction operation, and high sensitivity

Inactive Publication Date: 2008-04-09
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies in the prior art, provide a 2,4-D pesticide immunocolloidal gold labeling assay method, change the multi-step tedious detection of 2,4-D pesticide residues, and realize cheap, one-step rapid detection 2,4-D Pesticide Residue Method

Method used

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  • Colloidal gold chromatography semi-quantitative determination 2,4-D test paper and preparation method thereof
  • Colloidal gold chromatography semi-quantitative determination 2,4-D test paper and preparation method thereof
  • Colloidal gold chromatography semi-quantitative determination 2,4-D test paper and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0028] Adopt the improved EDC method to synthesize 2,4-D immune artificial antigen, with 2,4-DB as hapten, bovine serum albumin (BSA) as carrier protein, take by weighing 2,4-DB 60mg, add 0.05M PB ( pH 11 or above), stir to dissolve slowly, then adjust the pH value to about 7.2, add 60mg sulf-NHS, mix well, let stand for 5min, then add 90mg EDC, stir well, let stand for 5min, the reaction solution within 1 hour Slowly add it dropwise to 60mg 9ml of BSA solution, and stir the reaction at 4°C overnight, then dialyze 5-6 times with 0.15M NaCl solution, 8 hours / time, and then use the spectrometry method to determine the binding ratio of the conjugate to obtain the binding The 2,4-DB-BSA conjugate with a ratio of 1:24 was subpackaged and stored in a -20°C refrigerator. In the process of preparing the artificial antigen, the synthesis of the coated antigen is carried out at the same time. The method is to replace 2,4-DB with 2,4-D 60mg, and react with 60mg9ml OVA solution in the sam...

Embodiment 2

[0033] Adopt the improved EDC method to synthesize 2,4-D immune artificial antigen, with 2,4-DB as hapten, bovine serum albumin (BSA) as carrier protein, take by weighing 2,4-DB 60mg, add 0.05M PB ( PH 11 or above), stir to dissolve slowly, then adjust the pH value to about 7.2, add 60mg sulf-NHS, and mix well. Let stand for 5 minutes, then add 90mg EDC, stir well, let stand for 5 minutes, slowly drop the reaction solution into 60mg 9ml of BSA solution within 1 hour, and stir the reaction overnight at 4°C, then use 0.15M NaCl solution to dialyze 5- 6 times, 8 hours / time, after which the binding ratio of the conjugate was quantitatively determined by ultraviolet spectroscopic absorption method to obtain a 2,4-DB-BSA conjugate with a binding ratio of 1:24, which was subpackaged and frozen at -20°C in the refrigerator. In the process of preparing artificial antigen, the synthesis of coated antigen is carried out at the same time. The method is to replace 2,4-DB with 2,4-D 60mg, ...

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Abstract

The invention belongs to a test paper and a preparation method for quickly semi-quantitative testing the residual quantity of 2, 4-D in synthetic water samples, the invention relates to a GICA reaction which relies on a colloidal gold mark to show color, and the invention belongs to the immunochemistry measurement technical field. The theory of the invention is according to the reaction of 2, 4-D and the accurately quantitative gold mark antibody, and then the reactant has an emulous combination with the 2, 4-D-OVA which is cross-band-shaped distributed on the GICA film, and the content of the 2, 4-D of the sample is judged by the number of the stripe on the GICA film. The number is huge, the content of the 2, 4-D of the sample is high, the LOD of the 2, 4-D is 21ug / l, and the test can be finished in 10 minutes. The invention has practical character and remarkable progress, the traditional multi-step fussy detecting of the agricultural chemical is changed, and the method for quickly semi-quantitative testing the residual quantity of 2, 4-D in one-step is realized. The test paper stripe comprises a soleplate, an absorbent filter, a nitrocellulose membrane, a gold mark pad of anti-2, 4-D antibody and a sample adsorption pad, the nitrocellulose membrane in the middle of the soleplate is used for the experiment reacting region of a cellulose nitrate membrane, 4 detection lines and a quality control line are on the cellulose nitrate membrane, a sample area is on the far left, an absorbent filter is on the other end of the soleplate.

Description

technical field [0001] The invention belongs to immunochemical detection technology, specifically, an immunochromatographic reaction by means of colloidal gold color development, which is used for rapid detection of 2,4-D pesticide residues in agricultural products, water and soil. Background technique [0002] 2,4-D is a herbicide and plant growth regulator widely used in production. Its residual hazards in agricultural products, water and soil cannot be ignored. If its residues in soil and water exceed the standard, it is easy to cause cotton, certain vegetables, The poisoning of forest trees has caused great harm to human health. According to reports, 2,4-D has potential carcinogenicity and mutagenicity, and is an endocrine disrupting substance that has adverse effects on the immune system and reproductive system. After ingestion and absorption, it can also stimulate the eyes and skin, and after repeated exposure, it can damage the liver and heart, and can cause convulsio...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/532
Inventor 王升吉尚佑芬赵玖华杨崇良马立平崔汉青路兴波孙红炜袁雪英
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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