Colloidal gold immunochromatography test paper for antibodies of pseudorabies viruses of gE, gB and gD and preparation method

Abstract

The invention discloses a colloidal gold immunochromatography test paper for antibodies of pseudorabies viruses of gE, gB and gD and a preparation method. The test paper comprises a sample absorption area, a gold mark probe area, an immobilization antigen and antibody area, a water absorption area and a supporting plate, and the sample absorption area, the gold mark probe area, the immobilization antigen and antibody area and the water absorption area are laid on the supporting plate and are partially overlapped in sequence. The gold mark probe area is covered with gold mark probes which are mouse anti-swine IgG antibodies marked by colloidal gold. The immobilization antigen and antibody area is provided with a detection line T1 covered with pseudorabies virus gE protein, a detection line T2 covered with pseudorabies virus gB protein, a detection line T3 covered with pseudorabies virus gD protein and a control line C covered with goat anti-mouse IgG antibodies. The test paper is fast in detection, high in accuracy, strong in specificity, easy and convenient to carry and operate and capable of being used for differential diagnosis on PRV wild poisonous infection and vaccine immunity and evaluation on the PRV vaccine immunity effect at the same time.

Description

[0001] technical field [0002] The invention relates to a detection test paper and a preparation method thereof, in particular to a colloidal gold immunochromatographic detection test paper for porcine pseudorabies virus gE, gB and gD antibodies and a preparation method. Background technique [0003] Pseudorabies is an acute infectious disease caused by pseudorabies virus (PRV), including a variety of domestic and wild animals. Pigs are the main storage host and source of infection for pseudorabies. Healthy pigs can be infected with the disease by direct contact with sick pigs and infected pigs. Adult pigs are often insidiously infected; infected pregnant sows have symptoms such as miscarriage, stillbirth, weak fetus, and mummified fetuses; infected newborn piglets have fever and neurological symptoms, and even collapse to death, with a mortality rate of up to 100%. [0004] Since pseudorabies was first discovered in the United States in 1902, the disease has sprea...

AI Technical Summary

Problems solved by technology

Most of the reported PRV test strips can only detect antibodies against a single protein antigen (gE), and testers often need to use secondary detection to further determine whether there is PRV antibody in the absence of wild virus infection

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