Colloidal gold method detecting reagent for extrauterine pregnancy and number of pregnancy days, and preparation thereof

Abstract

The invention discloses an ectopic pregnancy and pregnant days colloid gold detection agent and manufacturing method thereof used for the problem of fast detection for pregnancy, comprising a piece of detecting test paper and urine sample treatment solution; wherein, the detecting test paper consists of a base plate, a cellulose nitrate membrane, a gold label anti-Beta HCG monoclone antibody layer, an Alpha-HCG monoclone antibody line, a sheep anti-rat polyclonal antibody line, an absorbing pad and a sample pad; the matching composition of the urine sample treatment solution is that: 12.5 to 18.0 portions of disodium hydrogen phosphate dodecahydrate, 1.2 to 1.7 portions of sodium dihydrogen phosphate dihydrate, 40.0 to 48.0 portions of sodium chloride and 4600 to 5300 portions of distilled water; the ectopic pregnancy and pregnant days colloid gold detection agent and manufacturing method of the invention are characterized by fast and simple detection, the operation of blood-drawing detection for 2 to 6 hours can be replaced by 2 to 3 minutes with time-saving, manual-saving and cost-saving; besides, the ectopic pregnancy and pregnant days colloid gold detection agent and manufacturing method thereof have the advantages of detecting ectopic pregnancy and pregnant days in a semiquantitative way, etc., and can be used for external detection of the pregnant condition and artificial abortion and assisted diagnosis.

Description

technical field [0001] The invention relates to a detection agent for detecting ectopic pregnancy and pregnancy days by colloidal gold method, that is, a biological immune method. Background technique [0002] The detection of ectopic pregnancy and pregnancy days is directly related to the health of pregnant women. Existing detection methods for ectopic pregnancy and pregnancy days are mainly blood test, ultrasonic detection, HCG detection and ultrasonic detection, etc., but all of them are time-consuming and complicated, requiring professional operation. In view of this, the usual OTC product colloidal gold early pregnancy detection test paper adopts double-antibody sandwich one-step technology, uses colloidal gold as an indicator mark, and detects the concentration of HCG in urine to determine whether it is pregnant, but its test results However, it is impossible to detect ectopic pregnancy and pregnancy time. People urgently need to develop a detection agent that can qui...

AI Technical Summary

Problems solved by technology

Existing detection methods for ectopic pregnancy and pregnancy days mainly include blood test, ultrasound detection, HCG detection and ultrasound detection, etc., but all of them are time-consuming and complicated, requiring professional operation

In view of this, the usual OTC product colloidal gold early pregnancy detection test paper adopts double-antibody sandwich one-step technology, uses colloidal gold as an indicator mark, and detects the concentration of HCG in urine to determine whether it is pregnant, but its test results However, it is impossible to detect ectopic pregnancy and pregnancy time. People urgently need to develop detection agents that can detect ectopic pregnancy and pregnancy days in an early and rapid manner.

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