AIDS (HIV-1/2) saliva rapid detection reagent

Abstract

The invention provides an AIDS (HIV-1 / 2) saliva rapid detection reagent, which is to fix a purified recombinant HIV-1 / 2 antigen on a fibrous membrane by adopting a principle of double antigen sandwich method. When the reagent is used, saliva to be detected is added. If a sample contains anti HIV-1 / 2 specific antibody, the antibody is combined with a corresponding antigen on the surface of the membrane to form a composite material. The composite material is captured by an antihuman-IgG monoclonal antibody to form a sandwich substance of the Au-HIVAg-HIV antibody-antihuman-IgG monoclonal antibody, and a purple strip is shown up. A negative sample cannot form the sandwich substance. The AIDS (HIV-1 / 2) saliva rapid detection reagent has high sensibility, better specificity and stability, has convenient operation without an instrument, and can detect AIDS virus in time by single portion. Detection processes can be reduced into one step and can be completed within 5 minutes, and the reagent can be applied to hospitals, epidemic stations and the Entry-Exit Inspection and Quarantine Bureau for field disease diagnose, and home self detection. The reagent uses saliva for directly detecting AIDS to avoid cross infection of blood and completely overcome the defect of laboratory detection technology, can carry out detection without a wound, has high speed, convenience and safety, and fills up domestic blank.

Description

1. Technical field [0001] The patent of the present invention relates to the technical field of medical immunodiagnosis, especially the saliva detection reagent of HIV. 2. Background technology [0002] (1) Immunocolloidal gold technology is a simple, fast and accurate detection technology developed in the 1990s. Its principle is similar to double sandwich ELISA, the difference is that the complex formed by colloidal gold binding protein is used as a probe. And the reagents used are all immobilized on the nitrocellulose membrane with micropores in advance. When using, place one end in the detection solution, and the nitrocellulose membrane can be passed through by the liquid, if the detection solution contains relevant antigens or antibodies. A light red line will appear in the detection area. Negative otherwise. Since the technology came out, it has been continuously adopted clinically. This method does not rely on sophisticated instruments, does not rely on skilled tech...

AI Technical Summary

Problems solved by technology

However, this method has many steps, high technical requirements, and the detection time is as long as 2 to 3 hours. The results need to be interpreted by instruments and the results cannot be saved.

These shortcomings limit its application and promotion at the grassroots level and in the field, let alone directly entering the family

(2) The development of AIDS colloidal gold diagnostic kits in my country started relatively late. According to the latest information released by the State Drug Administration, there are about 5 manufacturers in the country whose products (both using serum and plasma as test specimens) have passed the national inspection. ,

However, the above problems have not been completely resolved: 1). The blood collection process is traumatic and difficult to be accepted by the subjects; 2). There is a potential risk of contamination and diffusion during the blood collection process; 3). The detection method uses serum or plasma as a sample, Need to draw blood to separate serum or plasma, difficult to self-test at home