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Active carrier with fixed amino-compound content and fixing method thereof

A technology of active carrier and immobilization method, which is applied in the field of immobilization of amino acids or proteins on the surface of solid-phase materials, which can solve the problems of affecting the reliability of test results, shedding of antibodies or antigens, and poor uniformity, achieving good uniformity and simple production process , good stability

Inactive Publication Date: 2010-07-14
沙栩正 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2. Since the antibody or antigen is adsorbed on the surface of the carrier, over time, the antibody or antigen will fall off the surface of the carrier, thus affecting the reliability of the test result
[0006] 3. Since the antibody or antigen is adsorbed on the surface of the carrier, the uniformity of the same batch is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Add 160mL of chlorosulfonic acid, 40g of zinc chloride, and 640mL of toluene into the Erlenmeyer flask, and mix well; put the 96-well plate into it, and react at 30°C for 10.5 hours; take out the 96-well plate, rinse it with water, and then wash it with acetone lotion at 50°C Dry and store in a sealed container.

Embodiment 2

[0036] Add 85mL of chlorosulfonic acid, 80g of phosphorus oxychloride, and 450mL of toluene into the triangular flask, and mix well; put the 96-well plate into it, and react at 40°C for 7.5 hours; take out the 96-well plate and wash it with water, and then use acetone lotion for 60 ℃ drying, sealed storage.

Embodiment 3

[0038] Add 95mL of chlorosulfonic acid, 160g of zinc chloride, and 520mL of toluene into the Erlenmeyer flask, and mix well; put the 96-well plate into it, and react at 60°C for 6 hours; take out the 96-well plate, rinse it with water, and then wash it with acetone lotion at 40°C Dry and store in a sealed container.

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PUM

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Abstract

The invention discloses an active carrier with fixed amino-compound content and a fixing method thereof and in particular relates to a method for fixing amino acid or protein on the surface of the solid-phase material. The method comprises the following steps: high polymer with aromatic nucleus is adopted as a solid material; through chemical method, the high polymer is provided with functional group sulfonic acid chloride, the sulfonic acid chloride is reacted with a target substance containing amino, so the target substance is fixed on the surface of the solid polymer in the form of a chemical bond. The active carrier has simple production process and low cost. The fixing method saves raw material, has good stability and homogeneity, and can be used for the fields such as the radioimmunoassay, enzyme immnunoassay, chemiluminescence Immunoassay, time resolution immunity analysis, polymerase chain reaction, gene engineering, solid-phase synthesis of polypeptide, and the like.

Description

technical field [0001] The invention belongs to the technical field of immobilization of amino-containing compounds on the surface of solid-phase materials, especially the immobilization of amino acids or proteins on the surface of solid-phase materials, especially the immobilization of antigens or antibodies on solid-phase carriers. Immunoassay field of detection. Background technique [0002] Immunoassay diagnostic technology has the characteristics of high sensitivity, strong specificity, stable markers, etc., and its detection procedure is also simple and fast, requiring only a small amount of specimen. In recent years, it has been applied to detect various hormones, tumor markers, drugs and other trace amounts Bioactive substances can also be used for rapid diagnosis of bacterial and viral infections. [0003] In the currently applied immunoassay diagnostic technology, the carrier is a solid bead, tube, 96-well plate, etc. made of polystyrene as the main raw material. ...

Claims

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Application Information

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IPC IPC(8): G01N33/545C08J7/14
Inventor 沙栩正仲伟强
Owner 沙栩正
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