Norovirus nucleic acid detection kit and use method thereof

Abstract

The invention discloses a norovirus universal nucleic acid detection kit and a use method thereof. The norovirus universal nucleic acid detection kit comprises a norovirus reaction solution, a positive control solution and a negative control solution, and the norovirus reaction solution is freeze-dried powder which is pre-packaged into eight connecting tubes, wherein the norovirus reaction solution contains a plurality of probes with different fluorescence labels, primers, an enzyme mixture, an enhancer, a freeze-drying protective additive, a 10 * buffer, a nucleotide mixture and the like, and whether the norovirus is infected or not can be detected in the same reaction system. The whole process of sample collection, nucleic acid extraction, amplification and detection is monitored through the endogenous internal reference, and misjudgment caused by false negative results is avoided. The probes are oligonucleotide comprising a fluorescence reporter group and a quenching group. When the probes are complete, the quenching group is close to the reporter group in spatial position, so that fluorescence emitted by the reporter group is inhibited. When the primers extend, the probe combined with the template is cut off by Taq enzyme (5 '-> 3' exonuclease activity), the reporter group is separated from the quenching group, and a fluorescence signal is generated, so that the detection of the norovirus on the nucleic acid level is realized.

Description

Technical field [0001] The present invention relates to the field of nor, viral nucleic acid detection, particularly in a non-viral nucleic acid detection kit and method thereof. Background technique [0002] Norovirus (NV) is an RNA virus that causes intestinal acute disorders, according to the similarity of nucleotide sequences in the RNA polysesence zone, and divides NV into 5 genotypes (GI ~ GV), including GI, GII and GIV can be infected. Norgin virus has been considered by many countries to lead to the first pathogens of adult and child viral diarrhea and gastroenteritis, which are mainly in-manure-mouth, followed by direct contact infection, and China's popular human Nuo, like viruses mainly in Genome II as host. The clinical manifestation of infection is acute gastroenteritis and permeability of diarrhea. The course of disease is generally 7 days, and the fever lasts for 3 days, vomiting 2 ~ 3 days, diarrhea 5 days, severe dehydration symptoms. Nor is like a virus in cold ...

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a norovirus nucleic acid detection kit and its use method, aiming at the technical problems of slow detection of norovirus GⅠ and GⅡ virus detection and typing and identification, easy error and high cost

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