Nucleic acid detection kit for influenza A virus and influenza B virus and use method of nucleic acid detection kit

Abstract

The invention relates to a nucleic acid detection kit for an influenza A virus and an influenza B virus and a use method of the nucleic acid detection kit. The nucleic acid detection kit comprises an influenza A / B virus reaction solution, a positive control solution and a negative control solution, wherein the influenza A / B virus reaction solution contains a plurality of probes with different fluorescence labels, primers, an enzyme mixture, an enhancer, a freeze-drying protective additive, a 10xbuffer, a nucleotide mixture and the like, and can be used for detecting whether the A / B influenza virus is infected or not in the same reaction system. The whole process of sample collection, nucleic acid extraction, amplification and detection is monitored through the endogenous internal reference, and misjudgment caused by false negative results is avoided. When the probe is complete, a quenching group is close to a reporter group in spatial position, so that fluorescence emitted by the reporter group is inhibited. When the primer extends, the probe combined with a template is cut off by Taq enzyme (5 '-> 3' exonuclease activity), the reporter group is separated from the quenching group, and a fluorescence signal is generated, so that the influenza A virus and the influenza B virus are detected on the nucleic acid level.

Description

technical field [0001] The invention relates to the technical field of detection of type A and type B influenza viruses, in particular to a type A and type B influenza virus nucleic acid detection kit and a use method. Background technique [0002] Influenza is an acute respiratory infectious disease caused by influenza virus. It occurs frequently in spring and winter, spreads rapidly, has a high incidence rate, and has a short epidemic period. It is easy to cause outbreaks or pandemics, and it is difficult to control. Influenza viruses include three types: A, B, and C. Type A is the most likely to cause epidemics, followed by Type B, and Type C rarely causes epidemics. Influenza virus is mainly transmitted through air droplets, often causing fever, fatigue, muscle aches and mild to moderate respiratory symptoms, severe cases can cause pneumonia, myocarditis and heart failure. Because the infection symptoms and epidemic characteristics caused by these viruses are similar, i...

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Problems solved by technology

[0005] The invention provides a type A and type B influenza virus nucleic acid detection kit and a method of use, which solve the problems that the existing type A and type influenza virus detection is not convenient enough and difficult to evaluate the accuracy of the results

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