Primer, reagent kit and detection method of detecting short spine syndrome of dairy cattle

Abstract

The invention discloses a primer, a reagent kit and a detection method of detecting a short spine syndrome of dairy cattle and belongs to the field of molecular biology. The primer comprises a forwardprimer with a nucleotide sequence shown as SEQ ID NO (sequence identifier number): 1, and a reverse primer with a nucleotide sequence shown as SEQ ID NO: 2, wherein the forward primer is labeled withbiotin; and the reverse primer is labeled with digoxin. A recombinase-polymerase nucleic acid amplification technology and a lateral flow chromatographic technology are adopted; isothermal amplification is conducted on a clinical sample after DNA (deoxyribonucleic acid) extraction; a PCR (polymerase chain reaction) instrument is not required for a thermal circulation reaction; and a detection result can be directly read from a lateral flow chromatographic test strip by naked eyes. The primer has strong specificity and high amplification efficiency for detecting the short spine syndrome of thedairy cattle. The reagent kit and the detection method are simple in reaction procedure, can effectively and quickly detect the short spine syndrome of the dairy cattle and are applicable to simple,convenient, quick and accurate diagnosis of the short spine syndrome in a cattle farm.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a primer, a kit and a detection method for detecting short spine syndrome in dairy cows. Background technique [0002] Brachyspina syndrome (BS) was first reported in the Danish Holstein herd in 2006. It is a congenital recessive genetic fatal defect in dairy cows, which can lead to malformed embryos and even death or stillbirth. Short spine syndrome deficient calves may be severely underweight and stunted despite normal or long gestation periods. When stillborn calves are examined, a combination of severely shortened spines, long and thin limbs, and abnormal viscera are found. The molecular genetic mechanism for the formation of this symptom is a deletion mutation (Deletion) of 3329 bp in the FANC1 gene on bovine chromosome 21 (BTA21), resulting in the loss of exons 25, 26, and 27, and at the C-terminus of amino acid 877 The original 451 amino acid peptide chain is replaced by...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a primer, a kit and a test method for detecting short-spine syndrome in dairy cows, and solve the problem in the prior art that the detection of short-spine syndrome in dairy cows is cumbersome and difficult to meet the needs

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