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Propolis injection for preventing duck hemorrhagic oophoritis and preparation method thereof

A technology for hemorrhagic oophoritis and injection, which can be used in medical preparations containing active ingredients, sexually transmitted diseases, pharmaceutical formulas, etc., can solve problems such as virus spread, economic loss, and undiscovered, and achieve extended protection period and protection rate. improved effect

Inactive Publication Date: 2011-11-02
CHONGQING ACAD OF ANIMAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It brings great economic losses to the units or individuals that raise ducks and poultry. At the same time, due to improper handling of dead ducks and poultry, it often causes the spread of the virus and produces even greater consequences.
At present, the society has not yet found an effective drug that is highly effective and dedicated to the prevention of duck Tembusu virus disease, which is the most troublesome thing for duck and poultry breeding units or individuals

Method used

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  • Propolis injection for preventing duck hemorrhagic oophoritis and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Under aseptic conditions, the internal organs of ducks (including heart, liver, spleen, lung, brain and ovary) that died due to duck hemorrhagic ovariitis were taken and put into a tissue masher to mash, and 10% of the total weight was taken. The mashed duck tissue, which accounts for 90% of the total weight, is mixed with 0.8% normal saline to form a mixed tissue suspension, which is put into a centrifuge and centrifuged at 4 000 rpm for 20 minutes. After adding 2 000 units of penicillin and 2 000 units of streptomycin to the supernatant, duck hemorrhagic oophoritis seed poison was formed. Duck hemorrhagic oophoritis seed poison is put into concentration and is diluted 100 times in the physiological saline of 0.8%, forms duck yellow virus genus-Tembusu virus (Tembusu virus, TMUV) seed poison dilution, with 0.1 milliliter dose of duck tank The diluted solution of Busu seed poison was inoculated in the allantois of 9-day-old duck embryos. After the inoculation, the duck ...

Embodiment 2

[0024] Under aseptic conditions, the internal organs of ducks (including heart, liver, spleen, lung, brain and ovary) that died due to duck hemorrhagic ovariitis were taken and put into a tissue masher to mash, and 15% of the total weight was taken. The smashed duck tissue, accounting for 85% of the total weight, is mixed with 0.8% saline to form a mixed tissue suspension, and put it in a centrifuge and centrifuge at 4000 rpm for 30 minutes, and take the supernatant In the supernatant, 2000 units of penicillin and 2000 units of streptomycin were added to the supernatant to form duck virus oophoritis. Duck viral ovariitis seed poison is put into concentration and is diluted 100 times in the normal saline of 0.8%, forms the duck hemorrhagic ovariitis seed poison dilution, inoculates with the duck hemorrhagic ovariitis seed dilution of 0.1 milliliter dose in 11 days old In the duck embryo allantois of the duck embryo, select the duck embryo that dies in 80 hours of hemorrhage, ed...

Embodiment 3

[0026] Under aseptic conditions, the internal organs of ducks (including heart, liver, spleen, lung, brain and ovary) that died due to duck hemorrhagic ovariitis were taken and put into a tissue masher to mash, and 20% of the total weight was taken. The mashed duck tissue, accounting for 80% of the total weight, was mixed with 0.8% normal saline to form a mixed tissue suspension, and put it into a centrifuge and centrifuge it at 4000 rpm for 20 minutes, then take it Supernatant, after adding 2000 units of penicillin and 2000 units of streptomycin to each milliliter of supernatant, duck yellow virus seed virus was formed. The duck yellow virus seed virus is put into concentration and is diluted 100 times in the normal saline of 0.8%, forms the duck yellow virus seed virus dilution liquid, inoculates in 12 days old with the duck yellow virus virus seed virus dilution liquid of 0.1 milliliter dose. In the duck embryo allantois, after inoculation, select the duck embryos that died...

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Abstract

The invention relates to the field of animal prevention medicine, particularly to a preparation method for an injection for preventing animal epidemic diseases from occurring, wherein the propolis injection for preventing duck hemorrhagic oophoritis is composed of a propolis aqueous solution, a duck embryo homogenated tissue inoculated with duck flavivirus-Tembusu virus and dead by infection, andan inactivation solution; the preparation method comprises the step of adding the inactivation solution in the mixed solution of the duck embryo homogenated tissue and propolis to obtain the propolisinjection for preventing duck hemorrhagic oophoritis; the propolis injection for preventing duck hemorrhagic oophoritis prepared by the preparation method of the invention has a remarkable effect to prevent duck hemorrhagic oophoritis (duck Tembusu virus disease); the protection ratio is increased to 90-100%, the protection period is prolonged to 6-12 months, the action time is shortened to 5 days, and only once injection can achieve an expected effect.

Description

technical field [0001] The invention relates to the field of animal preventive medicine, in particular to a method for preparing an injection for preventing animal epidemics. Background technique [0002] Duck hemorrhagic oophoritis (duck yellow virus disease) is an acute infectious disease of breeding ducks and laying ducks caused by the flavivirus genus Tembusu virus (TMUV). It is characterized by a sudden and sharp drop in egg production. The disease mainly leads to follicular degeneration and deformation, follicular membrane hyperemia, and bleeding. The disease is temporarily called duck hemorrhagic ovariitis, which involves a wide range of areas, high morbidity, and a mortality rate of 5%-30%. The sick duck has fever and poor spirits. Grass-green diarrhea, unstable standing, paralyzed legs, stretching backwards or sideways; lesions are mainly seen in the ovary, manifested as ovarian dysplasia, follicle degeneration, deformation, follicular membrane hyperemia, bleeding, ...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61K39/12A61P15/00A61P31/14A61K35/28A61K35/30A61K35/34A61K35/407A61K35/42A61K35/48A61K35/26A61K35/54
Inventor 付利芝彭祥伟刘作华粟剑殷素会
Owner CHONGQING ACAD OF ANIMAL SCI
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