67results about How to "Extended protection period" patented technology

The invention relates to a method for producing a triple inactivated vaccine for newcastle disease, infectious bronchitis and infectious bursal disease. In the method, the inactivated vaccine is prepared by adopting a newcastle disease virus La Sota strain, an infectious bronchitis virus M41 strain, and an Escherichia coli genetic engineering bacteria E. coil BL21 / pET28a-VP2 strain which expresses an infectious bursal disease virus VP2 protein serving as production bacteria toxic species, a super concentration process and a high-quality adjuvant. After immunizing animals, antibodies are produced rapidly; the potency is high; the protection period is long; and the outbreak and the spreading of epidemic diseases are reduced.

The present invention discloses one kind of Brucellosis nucleic acid vaccine. The active component of the vaccine is one of the following mixture: 1. the mixture of at least two of the recombinant containing BCSP31 gene, the recombinant containing sodC gene and the recombinant containing L7 / L12 gene obtained through cloning BCSP31, sodC and L7 / L12 gene separately into eukarotic cell expression vector; and 2. the mixture of at least two of recombinant expression vectors obtained through fusing BCSP31, sodC and L7 / L12 gene separately with other nucleic acid sequence or deleting partial sequence or mutating partial nucleotides to obtain nucleotide sequence coding protein with the same activity as that of BCSP31, sodC and L7 / L12 gene and cloning separately to the eukarotic cell expression vector. The vaccine of the present invention may be used in preventing brucellosis of human body and animal.

The present invention is gaseous corrosion retardant for industrial apparatus and its preparation process. The supplied supermolecular structure gaseous corrosion retardant is intermolecular Al-Si compound-alkylamine-volatile amine associated complex in tunnel-shaped pore structure of different inner diameters. Two or more compounds are associated through intermolecular structure complementation and molecular distinction to reach the aims of regulating volatilizing property and protection radius. The preparation process of the gaseous corrosion retardant includes the first preparing Al-Si compound in tunnel-shaped pore structure and the subsequent gaseous or liquid phase association of alkylamine and volatile amine and the Al-Si compound to obtain the supermolecular structure gaseous corrosion retardant.

The invention belongs to the technical field of plastic processing, and particularly relates to an antibacterial polyethylene composition and an application thereof to preparation of an antibacterialfoamed packaging material meeting a fruit packaging demand. According to the foamed packaging material prepared by the antibacterial polyethylene composition, by exerting the function of a sterilizerand uniformly mixing the sterilizer with epoxidized soybean oil, calcium carbonate, calcium oxide and oleamide, a rate of absorbing water and moisture by calcium oxide can be controlled, so that calcium oxide plays a role slowly for a long time. Regardless of storage in a refrigerator or a problem that fruits decay and go bad as the storage conditions change after the fruits are stored and delivered, the antibacterial foamed packaging material has relatively good antibacterial protecting action to fruits, so that the storage effect of the fruits can be improved effectively, and the sterilizingand quality maintaining problems of the fruits can be solved better.

The invention relates to a Chinese herbal medicine additive for promoting growth of laying hens and improving egg quality and a feeding method thereof. The additive is prepared from the following Chinese herbal medicines in parts by weight of 2-4 parts of radix astragali, 1.5-3.5 parts of poria cocos, 1-3 parts of radix isatidis, 1-3 parts of radix codcnopsitis pilosulas, 1-2.5 parts of radix glycyrrhizae, 2-4 parts of fructus crataegi, 3-5 parts of rhizoma dioscoreae, 3-5 parts of fructus hordei germinatus, 1-2 parts of massa medicata fermentata, 1-2 parts of agastache rugosus, 1-2 parts of pericarpium citri reticulatae and 1-2.5 parts of herba ephedrae. Various Chinese herbal medicines are ground and sieved, and the sieved materials are mixed to obtain a mixture, namely the Chinese herbal medicine additive. 2 kg of the additive is added into every 100 kg of chicken compound feed according to the weight ratio to obtain chicken feed. 10-36 g of the chicken feed is used for feeding eachbrooding chicken every day, 40-80 g of the chicken feed is used for feeding each growing chicken every day, and 85-120 g of the chicken feed is used for feeding each laying hen every day. The additive has the characteristics of strong safety, small toxic and side effects and low residue, avoids tolerance of livestock and poultry to the additive, and basically realizes antibiotic-free breeding.

The invention relates to a method for producing chicken infectious bursal and avian adenovirus type 4 inactivated vaccines with LMH cell lines, which comprises the following steps: separately injecting chicken infectious bursal and avian adenovirus type 4 viruses Inoculate in LMH cells, obtain chicken infectious bursa seedling virus liquid and avian adenovirus type 4 seedling virus liquid by culturing, freezing and thawing, centrifuging, concentrating and other steps, and then sterilize the seedling virus liquid of the two viruses After inactivation, equal volumes are mixed and emulsified to make an emulsion-type inactivated vaccine. The dual vaccine provided by the invention has high potency, has complete immune protection against chicken infectious bursal virus and avian adenovirus type 4, has good safety, is stable and effective, and has a long protection period.

The invention discloses an anticorrosive coating, which is prepared by the following raw materials in parts by weight: 50-95 parts of alicyclic epoxy resin, 0.5-8 parts of a silane coupling agent, 1-7parts of a silicone intermediate, 1-2 parts of an antifoaming agent, 1-2 parts of a dispersing agent, 2-10 parts of a filler, 8-15 parts of a pigment, and 21-50 parts of a solvent. The silane coupling agent of the anticorrosive coating participates in the copolymerization of the alicyclic epoxy resin, improves the structural stability of the polymer, improves the corrosion resistance of the paintand the adhesion to an inorganic substrate, avoids spalling due to paint expansion at high temperature, and prolongs the protection period of the coating; the silicone intermediate is added, a silicon-oxygen bond is introduced into the structure, the heat resistance stability of the coating is increased, the hygroscopicity of the coating is reduced, and the coating has low cost and good performance.

The invention belongs to the technical field of veterinary biological products, and in particular to a dilution for a live vaccine of porcine reproductive and respiratory syndrome. Every 1000ml of phosphate buffer with the pH being 7 contains the following Chinese medicine polysaccharide components: 1-8g of folium mori polysaccharide, 0.1-5g of coriolus versicolor polysaccharide, 1-5g of rchmannia glutnosa polysaccharide and 0.1-5g of thymopentin. The dilution disclosed by the invention has the following beneficial effects: on one hand, the difference between the vaccine batches can be reduced and the overall immune level can be improved; and on the other hand, the protection period of vaccine can be prolonged, and the immune effect of the live vaccine for porcine reproductive and respiratory syndrome can be greatly improved.

The present invention is gaseous corrosion retardant for industrial apparatus and its preparation process. The supplied supermolecular structure gaseous corrosion retardant is intermolecular Al-Si compound-alkylamine-volatile amine associated complex in tunnel-shaped pore structure of different inner diameters. Two or more compounds are associated through intermolecular structure complementation and molecular distinction to reach the aims of regulating volatilizing property and protection radius. The preparation process of the gaseous corrosion retardant includes the first preparing Al-Si compound in tunnel-shaped pore structure and the subsequent gaseous or liquid phase association of alkylamine and volatile amine and the Al-Si compound to obtain the supermolecular structure gaseous corrosion retardant.

The invention belongs to the technical field of biological products for animals, and particularly relates to diluent for a live vaccine for hog cholera. 5g to 10g of agaricus blazei polysaccharide, 1g to 5g of herba andrographitis, 0.1g to 2g of ginsenoside Rh2 and 2g to 6g of curcumin are contained in every 1,000ml of a phosphate buffer solution, the pH (potential of Hydrogen) of which is equal to 7, of the diluent. The diluent for the live vaccine for the hog cholera, which is provided by the invention, is not only convenient to produce, low in cost and convenient and safe to use, but also can be used for improving the conversion ratio of a lymphocyte, decreasing a difference among vaccines of different batches and improving the immunization overall effect of the vaccines.

The invention discloses a traditional Chinese medicine immunopotentiator for a vaccine. The traditional Chinese medicine immunopotentiator for the vaccine is a mixed solution prepared from achyranthesbidentata polysaccharides, MEM dry powder, thymopeptide-5, HEPES and water. Per 1000 ml of traditional Chinese medicine immunopotentiator for the vaccine is prepared from 15 to 30g of achyranthes bidentata polysaccharides, 8 to 18g of MEM dry powder, 2 to 10g of thymopentin, 2 to 3g of HEPES and the balance of water. The invention also discloses a preparation method and application of the traditional Chinese medicine immunopotentiator for the vaccine. The traditional Chinese medicine immunopotentiator for the vaccine provided by the invention can regulate or enhance the body immunologic function, improve the body disease resistance capability and enhance the immune effect of the classical swine fever live vaccine; the preparation process is simple; the popularization is easy.

The invention discloses a Brucella nucleic acid vaccine. This brucella nucleic acid vaccine, its active ingredient is following any one mixture: 1) BCSP31, sodC and L7 / L12 gene are respectively cloned in the eukaryotic cell expression vector, obtain the recombinant expression vector containing BCSP31 gene, A recombinant expression vector containing the sodC gene and a recombinant expression vector containing the L7 / L12 gene, a mixture obtained by mixing the at least two recombinant expression vectors; 2) combining BCSP31, sodC and L7 / L12 with other nucleic acids After sequence fusion or partial sequence deletion or partial nucleotide mutation, the nucleotide sequences that can encode proteins with the same activity as BCSP31, sodC and L7 / L12 are respectively cloned into eukaryotic cell expression vectors, and the obtained at least two The mixture obtained by mixing the recombinant expression vectors. The brucella nucleic acid vaccine of the invention can be used for the prevention of human and animal brucellosis.

The invention relates to a method for producing a quadruple inactivated vaccine for newcastle disease, infectious bronchitis, avian influenza (H9 subtype) and infectious bursal disease. In the method, the inactivated vaccine is prepared by adopting a newcastle disease virus La Sota strain, an infectious bronchitis virus M41 strain, an avian influenza virus (H9 subtype) YBF003 strain, and an Escherichia coli genetic engineering bacteria E. coil BL21 / pET28a-VP2 strain which expresses an infectious bursal disease virus VP2 protein serving as production bacteria toxic species, a super concentration process and a high-quality adjuvant. After immunizing animals, antibodies are produced rapidly; the potency is high; the protection period is long; and the outbreak and the spreading of epidemic diseases are reduced.