Diluent for pig pseudorabies live vaccine

A technology of diluent and pseudorabies, which is applied in the field of diluents for porcine pseudorabies live vaccines, can solve the problems of unsatisfactory immune effect, short vaccine protection period, and little research on vaccine diluents, so as to prolong the vaccine protection period and reduce Immunization blank period, low cost effect

Inactive Publication Date: 2017-05-31
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in order to prevent and control porcine pseudorabies, a variety of porcine pseudorabies vaccines have been developed in my country, but there is little research on vaccine diluents. The immune effect of existing vaccine diluents is not ideal, and the vaccine protection period is relatively short

Method used

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  • Diluent for pig pseudorabies live vaccine
  • Diluent for pig pseudorabies live vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1, prepare the cistanche polysaccharide, comprising the following steps:

[0024] Add Cistanche deserticola powder to 0.5% Tween aqueous solution at a material-to-liquid ratio of 1:20, ultrasonically treat for 25 minutes at an ultrasonic power of 400W and a temperature of 40°C, and filter with suction. Cistanche polysaccharides can be obtained.

[0025] 2. The preparation of lentinan comprises the following steps:

[0026] (1) Remove impurities from the fruit bodies of fresh shiitake mushrooms, dry them at 60°C to constant weight, crush them at 24000r / min for 1-2min, and pass through a 40-mesh sieve to obtain shiitake mushroom powder;

[0027] (2) Weigh the shiitake mushroom powder obtained in step (1), add water for injection according to the liquid-to-material ratio of 30:1, mix well, extract with microwave under 800W power for 7-10min, filter with suction, and collect the filtrate;

[0028] (3) Repeat step (2) 2-3 times to collect the filtrate;

[0029] (4) The fi...

Embodiment 2

[0037] 1, prepare the cistanche polysaccharide, comprising the following steps:

[0038] Cistanche deserticola powder was added to 0.5% Tween aqueous solution at a ratio of 1:25 to liquid, ultrasonically treated for 30 minutes at an ultrasonic power of 500W and a temperature of 50°C, then filtered with suction, and the resulting filtrate was concentrated in a vacuum and processed by ethanol precipitation Cistanche polysaccharides can be obtained.

[0039] 2. The preparation of lentinan comprises the following steps:

[0040] (1) Remove impurities from the fruit bodies of fresh shiitake mushrooms, dry them at 60°C to constant weight, crush them at 24000r / min for 1-2min, and pass through a 40-mesh sieve to obtain shiitake mushroom powder;

[0041] (2) Weigh the shiitake mushroom powder obtained in step (1), add water for injection according to the liquid-to-material ratio of 38:1, mix evenly, extract with microwave for 7-10min at 880W power, filter with suction, and collect the...

Embodiment 3

[0051] 1, prepare the cistanche polysaccharide, comprising the following steps:

[0052] Add Cistanche deserticola powder to 0.5% Tween aqueous solution according to the ratio of 1:30 solid to liquid, ultrasonically treat for 40min under the conditions of ultrasonic power 600W and temperature 60℃, filter with suction, concentrate the obtained filtrate in vacuum, and process it by ethanol precipitation. Cistanche polysaccharides can be obtained.

[0053] 2. The preparation of lentinan comprises the following steps:

[0054] (1) Remove impurities from the fruit bodies of fresh shiitake mushrooms, dry them at 60°C to constant weight, crush them at 24000r / min for 1-2min, and pass through a 40-mesh sieve to obtain shiitake mushroom powder;

[0055] (2) Weigh the shiitake mushroom powder obtained in step (1), add water for injection according to the liquid-to-material ratio of 45:1, mix well, extract with microwave under 950W power for 7-10min, filter with suction, and collect the ...

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Abstract

The invention belongs to the technical field of veterinary biological products, and particularly relates to a diluent for pig pseudorabies live vaccine. Every 1000 ml phosphate buffer solution with the PH value of 7 contains 1-5 g of polysaccharide of cistanche deserticola, 60-80 mg of lentinan, 20-40 mg of paeoniflorin, 0.5-1 g of L-sodium glutamate, and 5-10 mg of phenol red. The diluent can effectively improve the immune effect of the pig pseudorabies live vaccine and prolong the vaccine protection term.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, in particular to a diluent for porcine pseudorabies live vaccine. Background technique [0002] Pseudorabies virus (PRV) belongs to Herpesviridae Alphaherpesvirus subfamily (Alphaher-pesririnae) varicella virus (Varicello virus) herpesvirus subfamily type I (Porcine herpesvirus Typel). Pigs are not only the reservoir of pseudorabies virus, but also the source of infection of pseudorabies virus. The control of porcine pseudorabies is of great significance not only to the pig breeding industry itself, but also to the smooth development of other animal breeding. In developed countries such as Europe and the United States, the disease has been controlled or eradicated thanks to the application of PRV gene-deleted live vaccines and corresponding identification methods. However, in developing countries, the disease still occurs frequently, which seriously restricts the developmen...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/12A61K47/36A61K47/26A61K47/18A61K47/22A61K9/08A61P31/22
CPCA61K39/39A61K9/08A61K39/12A61K47/183A61K47/22A61K47/26A61K47/36A61K2039/55511A61K2039/55577A61K2039/55583C12N2710/16734
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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