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Propolis injection for preventing type I duck virus hepatitis and preparation method thereof

A technology for duck viral hepatitis and injection, which is used in antiviral agents, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve the problems of high frequency of use, low protection rate, virus spread, etc., and achieve extended protection period. , the effect of improving the protection rate

Active Publication Date: 2011-11-02
CHONGQING ACAD OF ANIMAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Type Ⅰ duck viral hepatitis (hereinafter referred to as: duck viral hepatitis) is an acute infectious disease caused by a virus, which involves a wide range of areas and has a high incidence rate. At the same time, due to improper handling of dead ducks, the spread of the virus often results in greater consequences
At present, the main vaccines for the prevention of duck viral hepatitis have the problems of short immunization period (generally about 3 months), high frequency of use, long duration of action (14 days), and low protection rate (50-80%). Therefore, the harm brought to the poultry breeding industry is serious and causes economic losses

Method used

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  • Propolis injection for preventing type I duck virus hepatitis and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0019] Under aseptic conditions, the internal organs of ducks (including heart, liver, spleen, lung, brain) that died of duck viral hepatitis were taken and put into a tissue masher to mash, and 10% of the total weight was mashed. 90% of the total weight of the duck tissue was mixed with 0.8% saline to form a mixed tissue suspension, and it was placed in a centrifuge and centrifuged at 4000 rpm for 20 minutes, and the supernatant was taken. Add 2000 units of penicillin and 2000 units of streptomycin to the supernatant to form duck viral hepatitis seed virus. Duck viral hepatitis seed poison is put into concentration and is 0.8% physiological saline and is diluted 100 times in volume, forms duck viral hepatitis seed poison dilution, inoculates the duck of 9 days old with the duck viral hepatitis dilution of 0.1 milliliter dose In the embryo allantois, after inoculation, select duck embryos that died of characteristics such as hemorrhage and edema within 48 hours, put them into ...

Embodiment 2

[0021] Under aseptic conditions, take the dead duck viscera (comprising heart, liver, spleen, lung, brain) due to suffering from duck viral hepatitis and put them into a tissue masher to mash, and take 15% of the total weight to be mashed. 85% of the total weight of the duck tissue was mixed with 0.8% normal saline to form a mixed tissue suspension, which was put into a centrifuge and centrifuged at 4000 rpm for 30 minutes, and the supernatant was taken. Add 2000 units of penicillin and 2000 units of streptomycin to the supernatant to form duck viral hepatitis seed virus. Duck viral hepatitis seed poison is put into concentration and is 0.8% normal saline and diluted 100 times in volume, forms duck viral hepatitis seed poison dilution, inoculates duck of 11 days old with the duck viral hepatitis dilution of 0.1 milliliter dose In the embryo allantois, after inoculation, choose the duck embryos that died in the hemorrhage, edema and other characteristics in 80 hours after inocu...

Embodiment 3

[0023] Under aseptic conditions, take the duck internal organs (including heart, liver, spleen, lung, brain) that died of duck viral hepatitis and put them into a tissue masher to mash, and take 20% of the total weight to be mashed. Duck tissues accounted for 80% of the total weight were mixed with 0.8% physiological saline to form a mixed tissue suspension, which was put into a centrifuge and centrifuged at 4000 rpm for 20 minutes, and the supernatant was taken. Add 2000 units of penicillin and 2000 units of streptomycin to the supernatant to form duck viral hepatitis seed virus. Duck viral hepatitis seed poison is put into concentration and is 100 times of volume dilution in the normal saline of 0.8%, forms duck viral hepatitis seed poison dilution, inoculates in 12 days old with the duck viral hepatitis dilution of 0.1 milliliter dose In the duck embryo allantois of the duck embryo, select the duck embryo that dies in 48 hours of hemorrhage, edema and other characteristics ...

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Abstract

The invention relates to the field of animal prevention medicine, particularly to a preparation method for an injection for preventing animal epidemic diseases from occurring. The propolis injection for preventing type I duck virus hepatitis is composed of propolis aqueous solution, duck embryo homogenated tissue inoculated with duck hepatitis virus and dead owing to infection, and inactivation solution; the preparation method comprises the step of adding the inactivation solution in the mixed solution of the duck embryo homogenated tissue and propolis to obtain the propolis injection for preventing type I duck virus hepatitis; the propolis injection for preventing type I duck virus hepatitis prepared by the preparation method of the invention has remarkable effect to prevent duck virus hepatitis; the protection ratio is increased to 90-100%, the protection period is prolonged to 6-12 months, the action time is shortened to 5 days, and injecting once only can achieve the expected effect.

Description

technical field [0001] The invention relates to the field of animal preventive medicine, in particular to a method for preparing an injection for preventing animal epidemics. Background technique [0002] Type Ⅰ duck viral hepatitis (hereinafter referred to as: duck viral hepatitis) is an acute infectious disease caused by a virus, which involves a wide range of areas and has a high incidence rate. At the same time, due to improper handling of dead ducks, it often causes the spread of the virus and produces greater consequences. At present, the main vaccines for the prevention of duck viral hepatitis have the problems of short immunization period (generally about 3 months), high frequency of use, long duration of action (14 days), and low protection rate (50-80%). Therefore, the harm that poultry breeding industry is brought is serious, and causes economic loss. Contents of the invention [0003] One of the objects of the present invention is to provide an injection for ...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61K39/29A61P1/16A61P31/12A61K35/28A61K35/30A61K35/34A61K35/407A61K35/42A61K35/48A61K35/26
Inventor 杨金龙粟剑彭祥伟殷素会付利芝
Owner CHONGQING ACAD OF ANIMAL SCI
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