Cherry valley duck embryo epithelial cell line and establishment method thereof

Abstract

The invention discloses a cherry valley duck embryo epithelial cell line and an establishment method thereof, and belongs to the field of cytobiology. The method comprises the steps of taking duck embryo tissue with 9 to 12 age in days in a sterile way, shearing the duck embryo tissue into tissue blocks, adhering the tissue blocks downwards to the bottom of a culture hole, adding nutrient solution into a carbon dioxide incubator, and performing plate adhering culture at 37 DEG C. The high-survivability and high-purity cherry valley duck embryo epithelial cell line is obtained according to the method and conducted to biological preservation, compared with primary cell, the cell line has the advantages that the cellular morphology and the physiological property of the cell line are obvious different from that of the primary cell, continuous and stable passage can be realized, the nutritional requirement is low, the growth cycle is short, and the cell line is suitable for large-scale culture, provides a large amount of high-quality cell material for life sciences, such as gene engineering, cell engineering, immunology, molecular biology and the like, can serve as donor cell for poultry somatic cell clone breeding, and can also serve as a main material for duck virus isolation and vaccine production.

Description

technical field [0001] The invention relates to animal cell culture technology, in particular to a cherry valley duck embryo epithelial cell line and its establishment method. Background technique [0002] my country is the country with the longest history of waterfowl breeding and the largest breeding volume in the world. According to the statistics of the Food and Agriculture Organization of the United Nations, in 2008, the number of ducks in the world was 948 million, and China accounted for 661 million ducks, accounting for 69.7% of the world. It is a veritable duck-raising country. At present, the total annual production of ducks in the world has exceeded 2 billion, and the whole of Asia accounts for 85%, while China's production accounts for about 70%. have a major impact. [0003] Cherry Valley Duck is a duck species that was bred by hybridization of Peking Duck and Ellisbury Duck introduced from my country by the British Cherry Valley Farm. Its original species is ...

AI Technical Summary

Problems solved by technology

However, due to the slow growth of the primary cells of duck embryos, they are very sensitive to the culture environment, and the cell types are numerous and miscellaneous. Accelerated reproductive growth due to adaptation to the growth phase, while other cells either die or undergo gradual degeneration to death

The core problem of primary cell culture technology is that it is difficult to industrialize or scale production: one is that products cannot be prepared on a large scale during process production; the other is that non-batch production easily leads to inhomogeneity in product quality; Production for production and quality control

However, the culture method of static glass bottle or rotary bottle can no longer meet the required number of cells and their secreted products.

Although high-density cells can be obtained by using more reactor culture at present, it is difficult to expand the scale, and it is only suitable for a small amount of high-value cell culture

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