163results about How to "Suitable for large-scale cultivation" patented technology

The invention discloses a grape planting technique. The technique includes that before planting grape seedings, soil and grape roots are differently treated respectively, in other words, straw is laid on the soil, germicidal constituents like quicklime, paulownia sawdust, castor bean leaf powder and distillers' grains are scattered on the soil, the soil is covered by films after deep tillage and exposed to highlight, and harmful bacteria in the soil can be effectively killed, so that a good environment is provided for grape growing; the straw play a certain role in heat insulation, so that the grape roots can be prevented from cold injury in winter; the roots are dipped in slurry prepared by nitrogen-phosphorus-potassium mixed fertilizer, calcium superphosphate, urea, attapulgite, polyacrylamide, bamboo vinegar, sea mud and the like and then coated by a layer of plant ash, and the slurry has characteristics of slow releasing of the fertilizer and water retention, so that the number of fertilizing and watering can be effectively reduced, and grape planting cost can be reduced; after planting, straw powder and vermiculite are applied at the roots, soil can be improved, loss of the slurry coated at the grape roots can be prevented, and utilization rate of the fertilizer can be increased. According to the technique, safe and environment-friendly biological prevention and control methods are adopted for preventing and controlling plant diseases and insect pests, so that the plant diseases and insect pests are greatly reduced and safety and freeness of pollution are realized.

The invention discloses a high-quality and high-production cultivating technology of cane shoots, which comprises the steps of soil selection and preparation, germchit preparation, field planting, field management and harvest. According to the invention, before the field planting of cane shoots, sterilization treatment is performed on the soil by adopting quick lime, garlic dreg powder, tea bran powder and wood vinegar, so that the growing environment of cane shoots is purified, and the fertility of soil is improved at the same time; during the topdressing process, home-made slow-release organic granular fertilizer is applied, the fertilizer efficiency is more durable, and a large amount of fertilizer does not run away caused by climatic change, so that the fertilizer efficiency is greatly improved, the soil is improved at the same time, and the soil is prevented from hardening. The cultivating method is simple and feasible, low in cost, and high in yield coefficient; the cane shoots are convenient to manage, and applicable to large-scale cultivation; through large-area popularization, the quality and yield of cane shoots can be greatly improved and stabilized, so that the high-quality and high-production cultivating technology of cane shoots has prominent social, economic and ecological benefits.

The invention discloses a high-mountain konjak cultivation technique, which comprises the steps of land selection, soil preparation, breeding konjak selection and treatment, due-time planting, field management, diseases and pests prevention and control, harvest and the like. According to the invention, by soaking seeds with a Chinese herbal medicine solution, weeding with a Chinese herbal medicine herbicide, and spraying a Chinese herbal medicine disease and pest preventing agent for prevention, diseases and pests of the konjak can be greatly reduced, the growth environment of the konjak is purified, the use of chemical pesticide is reduced, and the ecological environment is protected. The konjak produced by the cultivation technique is good in product appearance, safe and nuisance-free. The cultivation method is simple, has the advantages of low cost, high yield and the like, and is convenient to manage and suitable for large-scale cultivation.

The invention discloses an artificial cultivation method of wild pinellia. The method includes the steps of land selection, land preparation, seed selection, seed soaking, timely seeding, field management, prevention and control of plant diseases and insect pests, harvesting, treating and the like. A self-prepared plant ash soaking solution is adopted for seed soaking, a good growing environment can be provided for pinellia tubers, plant diseases and insect pests can be reduced, and survival rate can be improved. A soil conditioner is applied during land preparation and base fertilizer applying, the soil conditioner can improve soil, break soil hardening, loosen soil, increase organic matters in the soil and promote soil microorganism activity, and can decompose animal and plant residues and trace metal elements in the soil to convert the same into nutrients of plants, serve as fertilizer, improve disease resistance of pinellia and promote quick growing of the pinellia. The artificial cultivation method is simple and easy to implement, simple and convenient in management, easy for growers to operate and implement, suitable for large-scale cultivation, high in yield and good in quality and completely meets market requirements.

The invention discloses an organic scented rice production technology. The production technology comprises the following steps of seed selection, seed processing, seedbed production, seeding, seedbed management and the like, so that produced organic scented rice is full in grain, stable in quality, safe and nonhazardous. The organic scented rice production technology is scientific, reasonable, simple, easy to operate for a farmer, simple in management, suitable for cultivation in large scale, and capable of improving social benefits, economic benefits and ecological benefits of the organic scented.

The invention discloses an early-spring high-yield potato cultivation technology. The early-spring high-yield potato cultivation technology comprises the following steps: variety selection, potato seed processing, grading-based germination acceleration, planting land selection, land preparation and fertilization, sowing and seedling raising, field management, control of pest and disease, harvesting and the like. According to the invention, by means of immersing seeds with a Chinese medicinal herbal solution and carrying out preventive spraying by using a Chinese medicinal herbal agent for preventing diseases and pests, disease and pest damages to potatoes can be reduced greatly, the growth environment of the potatoes is purified, the utilization of chemical pesticides is reduced, and an ecological environment is protected. The potatoes planted according to the invention are good in grade and are safe and pollution-free. The cultivation technology disclosed by the invention is simple, has the advantages of low cost, high yield, convenience for management and the like, and is suitable for large-scale cultivation.

The invention discloses a method for cultivating potatoes. The method comprises the following cultivating steps of selection of cultivating environment, arrangement of crops for rotation, variety choosing, pre-sowing preparation, field management, top dressing, control of diseases and insects, harvesting and the like. The method has the advantages of simplicity, low cost, high yield, suitability for large-scale cultivation and the like, and is convenient to manage.

The invention relates to a pollution-free grape planting method, comprising the steps of before field planting of a grape seedling, performing different treatments on soil and a grape seedling root; paving straws on the soil, then scattering quick lime, paulownia saw dust, castor leaf powder, vinasse and other sterilization components, deeply tilling the oil and laminating a film for hard light irradiation, so germs in the soil can be effectively killed, and a well environment is provided for grape growth; dipping the root with mud prepared from nitrogen, phosphorous and potassium compound fertilizer, calcium superphosphate, urea, attapulgite, polyacrylamide, bamboo vinegar and sea mud, and then coating a layer of plant ash, wherein the mud contains the slow-release fertilizers, and water retention, so fertilizing and watering times can be effectively reduced, and the grape planting cost is reduced; after field planting, applying straw powder and vermiculite to the root, thus improving the soil, preventing the loss of the mud coated to the root of the grape and improving the use rate of the fertilizers. The pollution-free grape planting method has the beneficial effect that a safe and environment-friendly prevention and control method is adopted for preventing and controlling diseases and insect pests.

The invention discloses a potato and cotton interplanting high-yield culture technique. The invention adopts a 2-1 planting mode in which light, heat, wind conditions in the crop population can be in preferable proportion, the potato and cotton yield-increasing potential can be realized to obtain high yield, and at the same time, the planting mode is also beneficial to plastic mulching, and saves the production cost. According to the invention, by broadcasting a Chinese herbal medicine solution to prevent soil insects, and spraying a Chinese herbal medicine disease and pest preventing agent to prevent diseases and pests, diseases and pests of potatoes and cotton can be greatly reduced, the growth environment of potatoes and cotton is purified, the use of chemical pesticide is reduced, and the ecological environment is protected. The potatoes and the cotton produced by the culture technique are good in product appearance, safe and pollution-free. The cultivation method is simple, has the advantages of low cost, high yield and the like, and is convenient to manage and suitable for large-scale cultivation.

The invention discloses a method for improving oil yield of microalgae. The method comprises the following steps: firstly, performing photoautotrophic cultivation on the microalgae in an improved culture medium containing a plant hormone, and at the stable phase, performing light stress and nitrogen stress cultivation on the microalgae obtained by centrifuging as seeds in a stress culture medium. The method for improving the oil yield of the microalgae has the advantages that the demand on nutrient substances in the cultivation process is reduced so that the cultivation cost can be reduced, and the biomass and the oil yield of chlorella can be improved simultaneously; the cultivation method is simple and easy to operate, and cannot be polluted easily; besides, the method has few influence factors and is suitable for large-scale cultivation.

The invention discloses a seedling growing method for spinach. The seedling growing method for spinach comprises the following concrete steps: sterilization; seed soaking; microwave treatment; ultrasonic treatment; magnetic field treatment and sowing. The seedling growing method is scientific, reasonable, simple, practicable and easy in management, and facilitates operation and implementation by growers; a transplanted plant has strong drought resistance; and spinach planted with the method is tender and delicious, high in nutritional value, suitable for large-scale cultivation, high in output and good in quality, and can totally meet market demands.

The invention discloses a cultivation method for high-yield potatoes. The method includes the steps of field selection, basal dressing, seed selection, sterilization and bud forcing, sowing, field management and prevention and control of insect pests and weeds. The cultivation method has the advantages of being simple, low in cost, high in yield, convenient to manage and the like, and is suitable for large-scale cultivation.

The invention belongs to the technical field of vegetable cultivation and particularly relates to a cherry tomato cultivation method. The cherry tomato cultivation method comprises the following steps of selecting vegetable plots, erecting protective greenhouses, raising seedlings in advance, preparing lands and performing timely transplantation, field management and fruit harvesting. The vegetable plot selecting step is that selected lands are plain, convenient to irrigate and drain, good in ventilation and light transmission and loose and fertile in soil or sandy soil and are plots where the same kinds of previous-crop vegetables are not planted. The protective greenhouse erecting step is that the protective greenhouses with the height of 3-4 meters are erected, automatic water spraying devices and temperature adjusting devices are arranged in the protective greenhouses, and heat-preservation films are arranged outside greenhouses. The cherry tomato cultivation method has the advantages of being simple, free of pesticide residues, environmental-friendly, free of pollution, high in yield, convenient to manage and the like and is suitable for large-scale cultivation.

The invention belongs to the field of agricultural biotechnology and relates to a method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches. The method comprises the steps of prothallium culture process, multiplication culture process, sporophyte induction process, rooting induction process, and transplant and manage process. The technological process is simple; by reproduction in the form of prothallium, a great amount of prothallium can be obtained; by the processes of multiplication, sporophyte induction, sporophyte rooting and transplant, a great number of seedlings capable of being used for production and culture can be obtained; the obtained seedlings are at the same physiological age and grow uniformly; and the method is suitable for industrial seedling raising and scale culture.

The invention discloses a high-yield planting technique for grapes. Before the grape seedlings are planted in the present invention, the soil and grape roots are treated differently: the soil is first covered with straw, and then sprinkled with bactericidal ingredients such as quicklime, paulownia sawdust, castor leaf powder, and distiller’s grains, and then covered with strong light after deep plowing. Irradiation can effectively kill harmful bacteria in the soil and provide a good environment for the growth of grapes. At the same time, the straw has a certain heat preservation effect and prevents the roots of the grapes from frostbite in winter; The mud made of calcium, urea, attapulgite, polyacrylamide, bamboo vinegar, sea mud, etc., is then covered with a layer of plant ash. The mud contains slow-release fertilizers and water-retaining properties, which can effectively reduce the cost of topdressing and watering frequency, reduce the cost of grape planting; after planting, apply straw powder and vermiculite to the roots, which can improve the soil, prevent the loss of the mud coated on the roots of the grapes, and improve the utilization rate of fertilizers; the present invention uses safe and environmentally friendly biological control methods to prevent and control diseases and insect pests , Pests and diseases are greatly reduced, safe and pollution-free.

The present invention relates to a rice cultivation method, and concretely relates to a rice cultivation method capable of effectively preventing diseases and pests. The method is characterized by comprising the steps of seedling cultivation, transplanting, field management and harvest. The method is scientific and reasonable in production technology, simple, easy to implement, easy for growers to operate and implement, simple and convenient to manage, and suitable for large-scale cultivation; organic rice is high in yield, good in quality, safe and pollution-free; social benefits, economic benefits and ecological benefits of the organic rice are raised; and a compound pesticide is adopted for preventive spraying, and traditional Chinese medicine bags are placed, thereby greatly reducing tryporyza incertulas insect attacks of rice, and purifying the growing environment of the organic rice.

The invention belongs to the field of veterinary parasites, and relates to coccidian oocyst culture preservative fluid and an application method thereof. The invention is characterized in that the culture preservative fluid takes polyhaxemethylenguanidine hydrochloride as a main ingredient. The application method of the coccidian oocyst culture preservative fluid comprises the following steps of: separating and purifying fresh coccidian oocysts from fowl dung or intestinal tract tissues containing the coccidian oocysts; adding an appropriate amount of a polyhaxemethylenguanidine hydrochloride solution; adjusting the density of the oocysts; controlling the depth of a culture medium; putting the culture medium in a shaking table to perform shaking culture; performing microscopic examination; stopping culture when the sporulation rate of the oocysts exceeds 85%; collecting the oocysts; adding an appropriate amount of the polyhaxemethylenguanidine hydrochloride solution again; and preserving in a refrigerator of which the temperature is 4 DEG C. The coccidian oocyst culture preservative fluid is efficient, long-acting and safe, pollution-free to the environment, simple to prepare and low in cost, and is ideal coccidian oocyst culture preservative fluid. By applying the application method provided by the invention, the operation is simple and convenient, the efficiency is high, the automation can be realized, and the coccidian oocyst culture preservative fluid is suitable for large-scale culture preservation of coccidian oocysts.

The invention discloses a standardized cultivation method of rhizoma bletillae, and belongs to the technical field of traditional Chinese medicinal material plantation. The standardized cultivation method of rhizoma bletillae comprises following steps: 1, seedling breeding; 2, field transplanting; 3, field cultivation growing period management; 4, harvesting and processing; and 5, packaging, storing, and transportation. Compared with the prior art, the standardized cultivation method possesses following advantages: 1, the standardized cultivation method is simple and practical, management is convenient, standardized technical specification is provided for steps from seedling breeding to packaging, storing, and transportation of rhizoma bletillae, and the standardized cultivation method is suitable for large-scale popularization and cultivation; 2, a set of rhizoma bletillae seedling breeding methods is provided, a defect of propagation by tuber that seed consumption amount is large is avoided, improvement of rhizoma bletillae seed germination induction, differentiation propagation, and rooting and strong seedling culture conditions is realized, rhizoma bletillae tissue culture plant survival rate is increased; 3, rhizoma bletillae seedling domestication cultivation measures are provided, and domesticated plant survival rate is increased; and 4, rhizoma bletillae culture medium formula ratio, bottle seedling, and domesticated plant seedling emergence standards are standardized, and lack of technology and standards in large-scale production is avoided.

The invention provides a macroporous micro-carrier with the liver cell specificity mainly made from SF / GC (Silk Fibroin / Galactosyl Chitosan) as well as a preparation method and the applications thereof. The macroporous micro-carrier is a sphere prepared from SF and GC under the action of a crosslinker, wherein the content of SF is 50-80 percent by weight of the sphere, and the content of GC is 15-40 percent by weight of the sphere, and the micro-carrier has the diameter of 200-500 microns and the aperture of 40-80 microns. The micro-carrier induces and improves the adhesion performance of the SF / GC on a macroporous micro-carrier support material to have the liver cell specificity and is suitable for culturing mass liver cells.

The invention discloses a glue-tenacity leather bacterium 10 tons liquid deep layer fermenting cultivation method, which belongs to edible fungus fermenting field. The technology process includes the following steps: bevel fungus active cultivating, first class seed cultivating, second class seed cultivating, third class seed cultivating, first class seeding tank cultivating, second class seeding tank cultivating, and fermentor cultivating. The cultivating carbon source of seed and fermenting is glucose, cane sugar, and starch. Nitrogen source is peptone, defatted soybean albumen powder, bean cake powder, and ammonium sulfate. The inorganic salts need to be added is potassium di-hydrogen phosphate, magnesium sulfate. Vitamin need to be added is vitamin B1. The invention establishes a good foundation for developing and process glue-tenacity leather bacterium.

The present invention relates to one kind of micro carrier for large scale cell culture. The micro carrier is prepared with chitosan and gelatin and through the following steps: mixing chitosan and acetic acid aqua and gelatin aqua in certain ratio to form composite chitosan-gelatin aqua; adding mineral oil with Span to form stable chitosan-gelatin water-in-oil preparation; adding glutaraldehyde to cure; filtering with 100 / 280 micron mesh and collecting solid micro carrier, or filtering with 450 / 600 micron mesh, freeze drying and collecting porous micro carrier; processing with glycine to close un-reacted aldehyde group; and finally processing with NaOH to eliminate residual acetic acid and to obtain the micro carrier. The micro carrier is favorable to cell adhesion and can maintain cell function for long time. In addition, the method of the present invention can control various parameters of the micro carrier in relatively wide range, has low cost and is suitable for industrial production.

The invention discloses a cultivation method of broad beans, which comprises the following steps of selecting and using a fine variety, selecting appropriate rotation crops, preparing soil well, applying a base fertilizer sufficiently, seeding, strengthening field management and harvesting. The cultivation method is simple, is suitable for large-scale cultivation, treats in a critical stage of growth and development of the broad beans, and comprehensively cultivates the broad beans by combining cultivation measures such as the scientific and reasonable fertilizer and the appropriate planting density, so that a yield of the broad beans is increased greatly, the quality is improved obviously, and contents of amino acid, a vitamin B and carotene are increased obviously.

The invention discloses a seedling raising method for summer squash. The seedling raising method comprises the following concrete steps: disinfection treatment, seed soaking treatment, microwave treatment, ultrasonic treatment and ultraviolet treatment. The method provided by the invention is scientific, reasonable, simple and practicable, is simple and convenient to manage, and facilitates operation implementation by growers; transplanted plants have strong drought resistance; and the planted summer squash has the advantages of fresh and tender quality, good taste, high nutritional value, applicability to large-scale cultivation, high yield, good quality, and capability of completely meeting the needs of market.

The invention relates to a preparation method of a recombinant protein, an indirect classical swine fever hemagglutination test kit that is prepared by the protein, and a preparation method of the test kit. The preparation method of the protein comprises following steps: a segment with the gene homeology degree comparing with a bovine viral diarrhea-mucosal disease virus (BVDV) that is lower than 35 percent is selected from a conservative region of a classical swine fever virus (CSFV) gene, thus obtaining a nucleotide sequence S1 that is corresponding to an amino acid A1; a forth amino acid in the nucleotide sequence S1 is modified to an hydrophilic amino acid, thus obtaining an amino acid A2; a corresponding nucleotide sequence S2 of A2 is modified, thus respectively obtaining three new nucleotide sequences; the three new nucleotide sequences are orderly and serially connected by connecting nucleotides, thus obtaining a sequence S9 that is corresponding to an amino acid sequence A3; and a nucleotide sequence S10 is obtained by processing S9 correspondingly, and after artificially synthesizing and digesting S10, connecting S10 with a carrier pGEX-4T-1 and inducing the recombinant carrier into colon bacillus for induction expression, the recombinant protein is obtained by separating and purifying an expression product.

The invention relates to a tomato cultivating method. The tomato cultivating method comprises the following steps of 1, performing sowing and seedling culture: performing seedling culture, performing transplanting, and soaking seeds in warm water; 2, performing soil preparation, and applying base fertilizers: generally making ridges at intervals of 4 chi; 3, performing field planting: transplanting seedlings; 4, performing field management, wherein during the period after the field planting and before seedling recovering, watering is not performed generally; 5, pruning branches and auxiliary buds, wherein pinching does not need to be performed on self-topping varieties, pinching needs to be performed on indeterminate growth type varieties, branches on a single trunk are pruned, namely only a main vine is reserved, and 4-5 flowers of each plant are reserved for pinching; and 6, preventing fruit dropping, too high temperature or too low temperature easily causes fruit dropping, so that flowers can be dipped with 2,4-D. The technical means of the tomato cultivating method disclosed by the invention has the beneficial effects that the tomato cultivating method is performed according to growth habits of tomatoes, environment required for the growth of the tomatoes is provided, the cultivated tomatoes are high in yield which can achieve 4000 kilograms / mu generally, besides, the licopersicin content is high, and requirements of people for nutrients of the tomatoes are met. The tomato cultivating method is reasonable in steps, and a great quantity of human resources and labor are saved.

The invention discloses a cultivation method for improving a survival rate of North American begonia and relates to the technical field of floriculture. The method comprises the following steps of 1, seedbed preparation; 2, cutting seedling preparation; 3, cuttage; 4, field planting; 5, field management. According to the cultivation method, the sprouting ratio and the survival rate are higher, the growth speed is high, the flowering period is long, and the method has good economic benefit and social benefit, and is suitable for large area cultivation and popularization.

The invention provides an infectious bursal disease virus Vero cell-adapted strain and belongs to the field of bioengineering. The related infectious bursal disease virus Vero cell-adapted strain is named Ck / Jiangsu / NJ-23 / 2008 and has a collection No. of CGMCCNO.8852. The infectious bursal disease virus Vero cell-adapted strain which can efficiently multiply on serum-free cultured Vero cell is finally obtained through wild strain separation, chick embryo passage, Vero cell passage adaption; the infectious bursal disease virus Vero cell-adapted strain is subjected to continuous passage culture on the serum-free cultured Vero cell and TCID50 can be kept to be higher than 108.5 / mL. Virus culture solution is inactivated and prepared into oil emulsion; after the prepared oil emulsion is used to immunize chicken, detection proves that the prepared oil emulsion has good immunogenicity. The infectious bursal disease virus (IBDV) strain and the production process thereof are simple, safe and efficient and suitable for industrial culture.

The invention discloses a pleurotus eryngii liquid strain with small and many mycelium pellets and a fermentation method of the pleurotus eryngii liquid strain, and belongs to the technical field of pleurotus eryngii cultivation. Chinese herb extracts capable of improving cold resistance, heat irritability resistance, immunity and bacterium-resisting capacity of the strain are scientifically compounded in culture mediums for liquid seeds, shaking flask seeds, seeding tank seeds and fermentation tank liquid strains in all stages, and meanwhile wood powder which has the feedstock adaptability and has the function of an antifoaming agent is scientifically compounded; manners of supplemental inoculation and segmentation variable temperature fermentation are adopted, and therefore the difference between the strain cell age and the multiplication algebra is shortened; and packaged chitosan with a special function is properly fed, and the pleurotus eryngii liquid strain with the thick and solid fermentative hyphae, high activity, small mycelium pellet diameter, large density and uniform distribution is cultured. The density of the mycelium pellets ranges from 4.5*10<5>piece / L to 5.5*10<5>piece / L, the diameter of the mycelium pellets ranges from 0.5mm to 0.7mm, the dry weight of the mycelium pellets ranges from 90g / L to 100g / L, the germination time ranges from 4 hours to 5 hours, and the exopolysaccharide ranges from 3.0g / L to 3.5g / L.

The invention discloses a club fungus cultivating method and a product thereof. The club fungus cultivating method comprises the steps that basal cell division is promoted while auricularia polytricha sporocarp development is interrupted by adjusting and controlling temperature and light intensity and cooperating with proper high-concentration CO2, top end cells of auricularia polytricha bases are simulated to continue to divide and growth upwards when normal cell division in auricularia polytricha basal bodies is up to a certain degree, so that the auricularia polytricha bases undergo the expanding, growing and proliferating process and bifurcate from the base portions, the middle-upper portions bifurcate for multiple times to form clusters, the top ends form finger-shaped cluster sets, finally coral grape vine shapes are formed, and the appearance, taste and nutritive value of club fungi are superior to those of club fungi of auricularia polytricha.

The invention belongs to the technical field of biology and relates to a preparation method and application of a novel antimicrobial peptide Misgurin mutant. The technical scheme is as follows: introducing glutamine at the C tail end of the amino acid sequence of natural Misgurin so as to design a novel Misgurin mutant; selecting Pichia yeast biased codons so as to artificially synthesize a complete gene of the novel Misgurin mutant; constructing a Pichia yeast secretion type expression vector pPICZaA-M; transforming a host cell SMD1168 by a vector electroporation method; selecting to obtain high-efficiency expression strains; and under proper culture conditions, ensuring 100 microgram of recombinant novel Misgurin mutant is contained in per milliliter of expression supernatant. The obtained product has a good nematocidal activity on staphylococcus aureus, pig salmonella and pig pathogenic Escherichia coli K88, K99 and 987P, and has an obvious effect on prevention and treatment of clinical bacterial diseases.