30results about How to "Help adhesion" patented technology

The present invention relates to one kind of micro carrier for large scale cell culture. The micro carrier is prepared with chitosan and gelatin and through the following steps: mixing chitosan and acetic acid aqua and gelatin aqua in certain ratio to form composite chitosan-gelatin aqua; adding mineral oil with Span to form stable chitosan-gelatin water-in-oil preparation; adding glutaraldehyde to cure; filtering with 100 / 280 micron mesh and collecting solid micro carrier, or filtering with 450 / 600 micron mesh, freeze drying and collecting porous micro carrier; processing with glycine to close un-reacted aldehyde group; and finally processing with NaOH to eliminate residual acetic acid and to obtain the micro carrier. The micro carrier is favorable to cell adhesion and can maintain cell function for long time. In addition, the method of the present invention can control various parameters of the micro carrier in relatively wide range, has low cost and is suitable for industrial production.

The invention relates to a preparation method of a mechanically-enhanced cell-loaded microchannel hydrogel, which comprises the following steps: preparing a mixed solution of hydrogel sol and cell culture fluid, and pouring the sol-cell mixed liquid into a polymethyl methacrylate die into which capillary tubes or fibers are inserted, thereby enabling primary crosslinking; drawing out the capillary tubes or fibers, introducing the hydrogel sol into microchannels of the cell-loaded hydrogel, and reinserting the capillary tubes or fibers, or sucking excess hydrogel sol out of the microchannels and carrying out ultraviolet irradiation, thereby enabling secondary crosslinking, so that the cell-loaded hydrogel with thick hydrogel layer is formed in the wall regions of the microchannels; and drawing out the capillary tubes or fibers, introducing endothelial cell culture fluid into the microchannels of the cell-loaded hydrogel, and culturing so that the endothelial cells grow and spread to form endothelial microchannels. The introduction of the thick hydrogel layer can maintain the stability of the microchannels in the hydrogel, and prevent shedding and intrusion of the endothelial cells;and the culture fluid in the microchannels can be filtered and screened.

The invention discloses a rapid preparation method for dry silver-stained samples of freshwater ciliates, belonging to the field of biotechnology. The method of the present invention comprises: (1) picking and collecting ciliates, and cleaning the surface of the ciliates; (2) adhering the clean worm liquid obtained from cleaning to the surface of a clean glass slide treated with polylysine, and removing the ciliate liquid Divide into several small drops and blow dry; (3) Add silver nitrate solution dropwise to the surface of the worm body, and clean the residue after standing; (4) Use a halogen cold light source to irradiate and develop; (5) Rinse the scum and dry it; (6) Add neutral gum dropwise to the surface of the worm body, add a cover slip and seal the slide. The method of the invention can efficiently and high-quality prepare dry silver-stained samples of freshwater ciliates, and at the same time greatly simplifies the film-making steps, and the invention does not need to use a developing solution, thereby saving raw material costs. In addition, the sample of the present invention is irradiated and developed under a cold halogen light source after being stained with silver nitrate, which is not affected by weather factors and is easier to control.

The invention relates to a protein adhered to the surface layers of bacteria and an application thereof. The invention discloses an application of an integration membrane protein STY in preparing a reagent for promoting the adhesion of cells. The invention also discloses a method for changing the adhesion property of the cells by utilizing the integration membrane protein STY. The invention also discloses a specific antibody of the integration membrane protein STY.

The invention discloses application of sodium hypochlorite in preparing a single-layer double-sided polypropylene / polydopamine patch. According to the application disclosed by the invention, by usingthe principles that sodium hypochlorite can quickly peel a polydopamine film from polypropylene and the polypropylene patch density is smaller than that of a saturated saline solution, a single-layerpatch is divided into two sides on nanoscale, one side is a polypropylene / polydopamine film face which can effectively prevent the patch from being attached to organs in the enterocoelia, and the other side is a polypropylene face which is exposed after the polydopamine nanofilm is peeled; the polypropylene face is internally in contact with peritoneum to improve abdominal wall tension, so that displacement risk is reduced, the structure of the patch is more reasonable, and requirements in the peritoneum and the enterocoelia are met simultaneously. Meanwhile, the preparation method is not related to stacked composition of varieties of composite materials, the thickness of the obtained patch is macroscopically kept as the thickness of a pure PP patch, and the patch is lighter and thinner and has a great clinical application value.

The invention belongs to the technical field of biological medicines, and particularly relates to a biomimetic composite patch. The biomimetic composite patch mainly comprises an isolating membrane, wherein holes are evenly distributed in two sides of the isolating membrane respectively; a cell induction layer is formed at one side of the isolating membrane; an outer layer space is formed at the other side of the isolating membrane; an induction space is formed in the middle of the isolating membrane; an impermeable layer covers the outer layer space, so as to provide a space for two-way growth of cells. The patch can absorb an electrospinning patch formed by a degradable material and a function factor complex. The invention provides a preparation method of the patch and use of the patch in the aspects of pasting, sealing, blocking, stopping bleeding, isolating, repairing and preventing adhesion of defective tissues in a surgical operation. The patch also can be used as a controlled release carrier of a medicament and a tissue engineering scaffold material.

The invention relates to a bionic in-situ regeneration repair nano sticking patch, which is formed by overlapping an layer A with a cationic group material and a layer B with an anionic group material sequentially, wherein the sticking patch comprises 20 to 80 percent by mass of the A layer with the cationic group material and the balance of the layer B with the anionic group material; layer A with the cationic group material comprises at least one of polysaccharide with a cationic group, protein with the cationic group or opypeptide (polypeptide) with the cationic group; layer B with the anionic group material comprises at least one of polysaccharide with an anionic group, protein with the anionic group or opypeptide (polypeptide) with the anionic group; and the sticking patch comprises not more than 10 percent by mass of added functional factors and / or functional polypeptide. The raw materials used by the invention are materials which can be degraded in vitro and have biocompatibility. The sticking patch is flexible, has good tissue adhesivity, is suitable for concave-convex surfaces of visceral organs, has strong stretching resistance and air pressure resistance, is suitable for application of sticking, sealing, leak stoppage, hemostasia, isolation, repair, adhesion prevention and artificial meninges of defect tissues and can also be used as a slowly-releasing carrier of a medicament and a nano-grade tissue engineering support material.

The invention provides an artificial dental planting body, which consists of a head part and a body part, wherein the body part comprises an inner side part, an outer side part and an anchoring part;the inner side part is a mandrel; the outer side part is a porous metal material sleeve and sleeves the mandrel; the anchoring part is fixed at one end of the mandrel; holes of the porous metal material of the sleeve are uniform; the length of projected ribs of the outer side surface of the sleeve is 30 to 50 percent of the pore diameter; the increase of friction between the surface of the porousmetal material and the peripheral bone tissue can be facilitated; the friction is uniform; the bone tissues are stimulated, and are promoted to uniformly grow into the porous metal material; the mandrel and the anchoring part prepared by the nanometer porous metal are favorable for the cell adhesion, differentiation and migration; and the storage of many growth factors and medicine is facilitated.The artificial dental planting body has the advantages that the uniform combination of the sleeve and the bone tissues can be realized; the fixation is firm; and the bone combination time is reduced.