Bionic in-situ regeneration repair nano sticking patch and preparation method and application thereof
An in-situ regeneration and patch technology, applied in the field of nano-patches, can solve the problems of too short degradation time in vivo, insufficient mechanical strength, loss of tensile strength, etc., to avoid toxic side effects and enhance mechanical properties.
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Embodiment 1
[0026] Example 1. Prepare a 12 cm diameter of the petri dish, and a built -in diameter polymer material film (was washed, sterilized, and removed thermal treatment) to dry;Shelling, 0.1mol / L acetic acid, 0.2 mol / L NaCl, pH = 4) and B Li (1 mg / ml carboxymethyl cylinder shell polycan, 0.15 mol / l naCl, pH = 6); Take part of the B Li add functional polypeptides (1 × 10 -4 Mg / ml) is made into C-liquid, and the function of the function of the function of the function is: lysine-lysine-cysteine-serine-arginine-glycine-illarine-cysteine-lymine-oligateAcids-lysine; pour the above solution to the high-pressure instant spraying equipment, first spray C solution at high pressure instantaneous when the dine is high-pressure; then spray A solution at a high pressure instantaneous instantaneous spray, rinse with water with water;Rinse with water with injections, so as to spray A, B, and rinse, and repeat 800 times; then spray A solution at a high pressure instantly, rinse with water with water; ...
Embodiment 2
[0027] Example 2. Prepare a 12 cm diameter of the petri dish, and a built -in diameter polymer material film (was washed, sterilized, and removed thermal treatment) to dry;Slutin, 0.1mol / L acetic acid, 0.2 mol / L naCl, pH = 4) and B liquid (2 mg / ml of alginic acid, 0.15mol / l naCl, pH = 6) referred to as B Li; Add some B liquid to the epidermal growth factor (EGF) (0.01mg / ml) to be made into a C -liquid; pour the above solution to the high -pressure instant spraying equipment, first spray C to the high -pressure instant C in the dine, dry the film into the film into the film into the film formation, the film forms the film into a film formation into a film formation.; Then spray A solution instantaneously, rinse with water with injection; then spray B liquid at high pressure, rinse with water with water, and alternately spray A liquid, B liquid, rinse, and repeat the operation 200 times; then high -voltage instant spray A solution, useRinse with water in injection; finally spray C s...
Embodiment 3
[0028] Example 3. Prepare a 12 cm diameter of the dine, a built -in diameter polymer material film (washed and sterilized and removed thermal treatment), washed and dried in injection;, 0.1mol / L acetic acid, 0.2mol / L NaCl, pH = 3.5) and B Li (1 mg / ml hyaluronic acid, 0.15mol / l naCl, pH = 6);Put it in the high -pressure instant spraying equipment, first spray A liquid at high pressure in the dine, dry, and then spray B liquid at high pressure instantaneous instantaneous spraying, rinse with water with water, so as to alternate A liquid, B liquid, rinse, and repeat the operation 500 times; finallyThen spray A solution at a high pressure, rinse with water with water, dry, unveil the film, rinse with water with injection, and dry the finished product.
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