53 results about "Macrophage proliferation" patented technology

Methods for modulating macrophage proliferation in an individual afflicted with or at risk for a macrophage-associated disease are provided. The methods employ a polyamine analog, or salt or protected derivative thereof. Macrophage proliferation has been implicated in a number of serious disorders, including AIDS (HIV)-associated dementia, AIDS-associated non-Hodgkin's lymphoma, and Alzheimer's disease. The invention also provides methods for aiding diagnosis and monitoring therapy of a macrophage-associated non-HIV associated dementia, especially Alzheimer's disease. The invention also provides methods of delaying development of macrophage-associated non-HIV associated dementias, including Alzheimer's disease, which entail administration of an agent which modulates macrophage proliferation.

Methods for modulating macrophage proliferation in an individual afflicted with or at risk for a macrophage-associated disease are provided. The methods employ a polyamine analog, or salt or protected derivative thereof. Macrophage proliferation has been implicated in a number of serious disorders, including AIDS (HIV)-associated dementia, AIDS-associated non-Hodgkin's lymphoma, and Alzheimer's disease. The invention also provides methods for aiding diagnosis and monitoring therapy of a macrophage-associated non-HIV associated dementia, especially Alzheimer's disease. The invention also provides methods of delaying development of macrophage-associated non-HIV associated dementias, including Alzheimer's disease, which entail administration of an agent which modulates macrophage proliferation.

Methods for modulating macrophage proliferation in an individual afflicted with or at risk for an ocular disease such as ARMD are provided. The methods employ a polyamine analog, or salt or protected derivative thereof. Macrophage proliferation has been implicated in a number of serious disorders, including ARMD. The invention also provides methods for aiding diagnosis and monitoring therapy of an ocular disease such as ARMD.

The invention discloses a preparation method of small-molecule panax notoginseng polysaccharide extract. The preparation method includes: using panax notoginseng as the raw material, crushing the raw material, extracting with an ethanol solution, extracting panax notoginseng residues with water obtain crude panax notoginseng polysaccharide extract, performing ethanol precipitation, dissolving precipitate with water, and then adding trifluoroacetic acid or lysozyme to perform hydrolysis to obtain crude small-molecule polysaccharide liquid; using a cation-anion exchange column to remove the protein, pigment and the like of the crude small-molecule polysaccharide liquid, using activated carbon powder for decoloring, filtering with a titanium-bar filter to obtain a colorless low-viscosity small-molecule polysaccharide solution, concentrating, and performing freeze drying to obtain the small-molecule panax notoginseng polysaccharide extract. The preparation method has the advantages that the method is simple to operate, low in cost and high in industrialization degree; the small-molecule panax notoginseng polysaccharide extract can promote macrophage proliferation, further differentiation and maturation of macrophage can be achieved to obtain activated macrophage, and the phagocytic ability of the macrophage can be evidently enhanced; fast increasing of cell immunity during a cell culture immunity test can be achieved; the extract has application prospect in immunological disease treatment, and the preparation method is suitable for industrial production.

The invention discloses application of long non coding RNA (lncRNA) and interfering RNA thereof to treatment on atherosclerosis. Through high-throughput sequencing on an aortic tissue of an atherosclerosis mouse model, the invention discovers that the expression of lncRNA molecules in normal and late atherosclerosis mice is obviously different; and meanwhile, the lncRNA molecules are highly expressed in the atherosclerosis tissue and the lncRNA molecules are named as lncRNA-AFIAR. The experiment proves that the lncRNA-AFIAR has the functions of enhancing proliferation of macrophages, inhibiting macrophage apoptosis and the like and participates in the process of promoting the progress of the atherosclerosis. The aims of inhibiting the proliferation of the macrophages and reducing plaque formation can be fulfilled by inhibiting the lncRNA-AFIAR, so that the aim of inhibiting the progress of the AS. Therefore, the invention provides new molecular markers and intervention targets for diagnosing and treating the atherosclerosis and also provides new technical means for treating the atherosclerosis.

The invention relates to the processing field of a selenium-rich rice by-product, in particular to a preparation method of rice enzymolysis selenium polypeptides with immune activity. According to the method, selenium-rich rice processing by-product broken rice is used to prepare degreased selenium-rich rice powder by grinding, sieving and degreasing, a supernatant is collected after alkaline extraction and centrifugation by using a sodium hydroxide solution, a dilute hydrochloric acid solution is used for acid precipitation, and after centrifugation, a precipitate is taken for washing and then is frozen-dried. The obtained rice proteins, after enzymolysis by using trypsase, are frozen-dried to obtain the rice enzymolysis polypeptides with the selenium content of 0.3-0.4mg / kg. In a cell immune function experiment, the rice enzymolysis selenium polypeptides have good capacity for promotion of cell proliferation and neutral red phagocytosis of macrophage. The rice enzymolysis selenium polypeptides are natural in nutrition, rich in trace element selenium, and suitable to use as auxiliary food materials, nutrient supplements or health food for enhancing immune functions of bodies, and the preparation method is simple and reliable.

The invention discloses a polymer / silver active bonding compound for inhibiting the proliferation of activated macrophage, and a preparation process and applications of the bonding compound. The polymer / silver active bonding compound consists of nano elemental silver and a hydrophilic polymer modified by a target head. The polymer / silver active bonding compound is excellent in stability and can inhibit the proliferation of activated macrophage, so that the polymer / silver active bonding compound is good in anti-rheumatoid arthritis effect in vitro and in vivo.

The invention discloses ginseng derived nano-particles, as well as a preparation method and application thereof. The ginseng derived nano-particles have a membrane structure, wherein the particle size range is 150 to 500nm, and the peak particle size is 280 to 350nm. The ginseng derived nano-particle is prepared by performing centrifugation and filtering impurity removal for many times. By the ginseng derived nano-particle, a bone marrow derived monocyte-macrophage can be effectively induced to be proliferated and activated, meanwhile, multiple surfactant molecules such as a TLR2 / 4 (toll-like receptor 2 / 4) and a CD80 (cluster of differentiation 80) are up-regulated, and cell factors such as a TNF-a (tumor necrosis factor-a) and an IL-6 (interleukin-6) are secreted. The ginseng derived nano-particle has broad application prospect in development of a medicament for preparing a natural immunoenhancer.

The invention discloses a mutant strain of a mycoplasma bovis Mbov_0475 gene, which belongs to the technical field of animal infectious disease prevention. The mutant strain is named as Mycoplasma bovis T9.55, and is preserved in China Center for Type Culture Collection, a preservation number is CCTCC NO: M 2020082, and the nucleotide sequence of the Mbov_0475 gene is shown as SEQ ID NO: 1. The strain has the effects of inducing macrophage proliferation and improving macrophage activity, and because bovine macrophages are important immune cells of a host for resisting pathogenic infection, themutant strain is expected to play an important role in the field of mycoplasma bovis immune prevention and treatment.

The invention provides an M1 type MAP (macrophage activation peptide) and IL-2 (interleukin-2) fusion protein and fusion gene as well as an expression vector and a construction method of the expression vector. The amino acid sequence of the fusion protein is shown as SEQ ID NO:1. A multi-expression M1 type MAP and an IL-2 gene are adopted for splicing coexpression and constitute key factors for activating immune response. The fusion protein can perform a function and play a role simultaneously in intestinal mucosa and mesenteric lymph nodes and has remarkable treatment and regulation functions on PRRSV and PCV2, the quantity of M2 type macrophages is reduced by stimulating proliferation and activation of M1 type macrophages, and immunosuppression is recovered effectively.

The invention relates to a preparation method for Sinkiang turnip polysaccharide having immunoregulatory activity. The method includes the steps that Sinkiang turnip root tubers are dried and smashed, ethyl alcohol with the concentration of 80% is used for removing impurities from the Sinkiang turnip root tubers, the Sinkiang turnip root tubers are subjected to hot water extraction, suction filtration is conducted to extract supernate, absolute ethyl alcohol precipitation and centrifugation are conducted, precipitate is washed sequentially through diethyl ether, ethyl alcohol and acetone and is dried until the constant weight state is reached, and the Sinkiang turnip polysaccharide is obtained. An MTT method is used for analyzing the immunoregulatory activity of the Sinkiang turnip polysaccharide and it is discovered that the Sinkiang turnip polysaccharide has a remarkable effect of promoting mouse peritoneal macrophage proliferation. The preparation method for the Sinkiang turnip polysaccharide has the advantages that the extraction yield is high, cost is low, the structure of the polysaccharide is complete, and no pollution is caused. The prepared Sinkiang turnip polysaccharide has the prominent immunoregulatory activity, thereby having broad application prospects in the food industry and the pharmaceutical industry.

The invention discloses a fusarium oxysporum water extract and a function and an application of a polysaccharide thereof on the aspect of immunological enhancement, which belong to the field of bio-pharmaceuticals. The fusarium oxysporum water extract and the polysaccharide thereof have remarkable immunological enhancement effects, and can be used for promoting macrophagocyte proliferation, improving the macrophagocyte cytophagy function and activating release of immune active factors NO, IL-1alpha and TNF-alpha by macrophagocytes. The fusarium oxysporum water extract and the polysaccharide can be used for assisting in treating immunocompromise diseases and tumors under an immunological enhancement action.

The present invention provides a remedy for a nerve dysfunctional disorder such as a central nervous system damage including a spinal cord injury and a cerebral infarction and the like having an excellent nerve regeneration promoting action which can be administered not only by injecting into a injured site but also by various administration methods including intravenous administration, which can be easily handled and stored over a long time, and can be prepared in a large amount at any time. Said remedy for a nerve dysfunctional disorder such as a central nervous system damage including a spinal cord injury and a cerebral infarction and the like are prepared by using the following as active ingredients: one or more substances selected from a substance secreted from dendritic cells such as IL-12, GM-CSF and the like, a substance inducing and proliferating dendritic cells, a substance activating dendritic cells; a substance inducing the expression of a neurotrophic factor in nerve tissues, a substance inducing and proliferating microglias and macrophages in nerve tissues; and a vector which can expresses the aforementioned substances; or dendritic cell subsets secreting a neurotrophic factor such as NT-3, CNTF, TGF-β1, IL-6, and EGF.

The invention discloses gastrodia elata macromolecule linear straight-chain glucan and a preparation method and application thereof, and belongs to the field of traditional Chinese medicine. The method comprises the steps: drying gastrodia elata tubers, smashing and degreasing with ethyl alcohol; extracting at room temperature, centrifuging and concentrating an extracting solution, carrying out alcohol precipitation, precipitating and redissolving, dialyzing by adopting a dialysis bag with the molecular weight cutoff of 8000-14000 Da, concentrating the dialyzed solution, and further separatingand purifying by using ion exchange resin and gel exclusion chromatography to obtain the gastrodia elata macromolecular linear straight-chain glucan. The polysaccharide is only composed of glucose, is alpha-D-1, 4 connected homopolydextran, and is a new dextran. When the concentration is 400 [mu] g / mL, macrophage proliferation can be significantly promoted, the phagocytic ability of macrophages can be enhanced, the macrophages can be promoted to release NO and cytokines TNF-alpha and IL-6, and the body immunity can be enhanced.

The invention discloses an enterococcus exopolysaccharide with an immunomodulatory effect as well as a preparation method and application of enterococcus exopolysaccharide; enterococcus hiraei WEHI01 is used for fermentation to generate the exopolysaccharide, the exopolysaccharide is separated and purified, and the enterococcus hiraei WEHI01 is preserved in the China Center for Type Culture Collection, and has the preservation number of CCTCC NO:M 2019068. The prepared enterococcus exopolysaccharide comprises four components: EPS-I01, EPS-I02, EPS-I03 and EPS-I04, the average molecular weight of the component EPS-I02 is 2.28*10<4> Da, and the component EPS-I02 is composed of rhamnose, trehalose, arabinose, xylose, mannose, glucose and galactose according to the molar ratio of 1:1.76:5.48:1.76:2.52:6.2:20.92. The enterococcus exopolysaccharide prepared by the preparation method has an immunoregulation effect, can significantly improve the proliferation rate and phagocytic ability of macrophages, and significantly promote macrophages to secrete NO and cell factors, and the enterococcus exopolysaccharide as an additive is applied to the fields of food and medicine, and has a wide application prospect.

The invention relates to an application of lactobacillus acidophilus S-layer protein in intestinal cell adhesion and macrophage proliferation. An extraction method of the lactobacillus acidophilus S-layer protein comprises the following steps of destroying hydrogen bonds on the surface of lactobacillus acidophilus cell walls by using neutral 4-5mol / L lithium chloride, and purifying by gel filtration or ion exchange chromatography, to obtain the lactobacillus acidophilus S-layer protein. The lactobacillus acidophilus S-layer protein is beneficial to adhesion of lactobacillus acidophilus to intestinal cells, the adhesion function of lactobacillus acidophilus to intestinal cells is reduced along with extraction and separation of the S-layer protein, and the lactobacillus acidophilus S-layer protein is also beneficial to macrophage proliferation and macrophage lysosome secretion within a proper concentration range, and cell autoimmunity enhancement is realized.

The present invention provides sea cucumber polypeptides and an application thereof, and belongs to the technical field of biological pharmacy. The provided sea cucumber polypeptides can be used for preventing or treating diseases related to macrophages, including but not limited to body local tissue infection or lesion and autoimmune diseases caused by pathogenic microorganisms such as viruses and the like, by promoting proliferation of the macrophages, ; Meanwhile, the sea cucumber polypeptide can also be used as a macrophage proliferation promoter to promote in-vitro culture of macrophages.A plurality of the sea cucumber polypeptides with activity of promoting macrophage proliferation are artificially synthesized, so that the sea cucumber polypeptides have good practical application value.

The invention relates to an organism modified preparation method for improving the activity of lily polysaccharides, belonging to the field of agricultural-product functional ingredient preparation technologies. Lily polysaccharides (403.3kDa) are subjected to organism modification (namely enzyme-method controllable degradation) by adopting a compound enzyme (beta-mannanase and beta-glucanase) method. After the modification, the lily polysaccharides are subjected to separation and purification, and the molecular weight of product lily polysaccharides is 8.0-70.0kDa. The in-vitro immunoregulation activity of the lily polysaccharides and modified products of the lily polysaccharides is subjected to model research by adopting in-vitro macrophages RAW264.7. The activity of the modified lily polysaccharides is improved remarkably, and the macrophage proliferation activity is increased by 40.5-77.2% compared with a substrate. The activity of the lily polysaccharides is relatively low no matter the molecular weight of the lily polysaccharides is too high or too low. The method for improving the immunocompetence of the lily polysaccharides is created, and the biologically modified lily polysaccharides have great application values in the fields of medicines and functional foods.

Methods for modulating macrophage proliferation in an individual afflicted with or at risk for an ocular disease such as ARMD are provided. The methods employ a polyamine analog, or salt or protected derivative thereof. Macrophage proliferation has been implicated in a number of serious disorders, including ARMD. The invention also provides methods for aiding diagnosis and monitoring therapy of an ocular disease such as ARMD.

The invention provides application of rMBP-NAP in regulation and control of a macrophage. The inventor accidently finds that the MBP-NAP can promote proliferation of the macrophage and / or enhance pinocytosis capability of the macrophage and / or promote the macrophage to be polarized toward an M1 type. The invention also provides application of MBP-NAP fusion protein in direct tumor resistance.

The invention provides a novel reagent applicable to treatment of malignant cerebral malaria. The reagent is composed of a cavity protein shell formed by self-assembling of 24 protein subunits and aniron-based nano-enzyme with catalase activity, and can specifically target brain microvascular endothelial cells and remove ROS. Since an iron ion channel is formed in the protein shell of the reagent, nanometer iron core with uniform particle size can be synthesized in a cavity of the protein shell, and the nanometer iron cores have catalase catalytic activity and can catalyze decomposition of hydrogen peroxide; and the ferritin shell can target cerebral endothelial cells, promote proliferation of macrophages in the liver and polarization of the macrophages to the M1 subtype, and enhance thephagocytic function of the macrophages on infected red blood cells. Therefore, the reagent can be used for treating cerebral malaria.

The invention discloses polysaccharide SM-W, which is separated from raspberry polysaccharide, a specific sugar chain structure of the polysaccharide SM-W is identified, and tests prove that the polysaccharide SM-W can promote macrophage proliferation, promote macrophage to release NO and up-regulate expression of TNF-alpha and IL-6 so as to enhance phagocytic action of macrophage and improve immunity. The polysaccharide SM-W is also beneficial for macrophage to play an anti-tumor role, can be used for preparing related products, and provides reference for further exploring the structure-activity relationship of the raspberry polysaccharide and developing polysaccharide anti-tumor drugs and immunologic adjuvants.

The invention discloses a polysaccharide SM-0.4M which is separated from raspberry polysaccharide. A specific sugar chain structure is identified, and tests prove that the polysaccharide SM-0.4M can promote macrophage proliferation, promote macrophage to release NO and up-regulate expression of TNF-alpha and IL-6, thereby enhancing phagocytic action of macrophage and improving immunity. The anti-tumor effect by themacrophages is facilitated, related products can be prepared, and reference is provided for further exploring the structure-activity relationship of the raspberry polysaccharide anddeveloping polysaccharide anti-tumor drugs and immunologic adjuvants.

The invention relates to the field of food processing and application, in particular to sea intestine gum as well as a preparation method and application thereof. Collagen from sea intestines is extracted by adopting an enzyme method, and a concentrated gel product is further prepared. Activity of the product is evaluated by measuring a proliferation activity of the product on macrophages, and it is found that the product can significantly promote proliferation of the macrophages. The sea intestine gum and the preparation method thereof not only prolong shelf life of sea intestines, but also facilitate further development of sea intestine products, increase resource utilization, and provide a basis for future sea intestine research and resource development.

The invention discloses a macrophage induced culture medium and a macrophage culture method. The macrophage induced culture medium contains a GM-CSF (granulocyte-macrophage colony stimulating factor), plasma and a serum-free basal culture medium. The macrophage culture method includes using tumor cell lysate supernatant for stimulating macrophages. The macrophage induced culture medium and the macrophage culture method have the advantages that a specific concentration of GM-CSF and the plasma are used for inducing CD14<+>cells and the tumor cell lysate supernatant is used for stimulating the CD14<+>cells, so that macrophage proliferation is promoted, and macrophage purity is also improved.

The invention relates to an organism modified preparation method for improving the activity of lily polysaccharides, belonging to the field of agricultural-product functional ingredient preparation technologies. Lily polysaccharides (403.3kDa) are subjected to organism modification (namely enzyme-method controllable degradation) by adopting a compound enzyme (beta-mannanase and beta-glucanase) method. After the modification, the lily polysaccharides are subjected to separation and purification, and the molecular weight of product lily polysaccharides is 8.0-70.0kDa. The in-vitro immunoregulation activity of the lily polysaccharides and modified products of the lily polysaccharides is subjected to model research by adopting in-vitro macrophages RAW264.7. The activity of the modified lily polysaccharides is improved remarkably, and the macrophage proliferation activity is increased by 40.5-77.2% compared with a substrate. The activity of the lily polysaccharides is relatively low no matter the molecular weight of the lily polysaccharides is too high or too low. The method for improving the immunocompetence of the lily polysaccharides is created, and the biologically modified lily polysaccharides have great application values in the fields of medicines and functional foods.

The invention provides a method for verifying the Eltrombopag (ELB) mediated anti-angiogenesis function of macrophage. Through MTT assay, q-PCR and ELISA (enzyme linked immunosorbent assay), influenceof the ELB upon macrophage proliferation, expression of mRNA (messenger ribonucleic acid) of related angiogenesis factors (VEGF-A, bFGF, MMP-9) and VEGF (vascular endothelial growth factor) proteinscan be detected; and furthermore, through MTT experiments, cell wound scratch assay and Matrigel pipe formation experiments, influence of the ELB upon endothelial cell proliferation, migration and tube cavity formation through macrophage can be observed. The method verifies that the ELB is capable of inhibiting proliferation of the macrophage, inhibiting mRNA of the expression of related angiogenesis factors and inhibiting release of the VEGF proteins, and meanwhile, finds that the ELB acts on supernate of the macrophage and is capable of remarkably inhibiting proliferation, migration and tubule formation of the endothelial cells, and thus the macrophage can be mediated by the ELB and thus has the anti-angiogenesis function.

The invention discloses a polysaccharide SM‑0.2M, which is separated from raspberry polysaccharides, and its specific sugar chain structure is identified. It is verified by experiments that the polysaccharide SM‑0.2M can promote the proliferation of macrophages, and can promote macrophages The cells release NO, up-regulate the expression of TNF-α and IL-6, and then enhance the phagocytosis of macrophages, improve immunity, and help macrophages play an anti-tumor effect, which can be used to prepare related products, and to further prove The structure-activity relationship of raspberry polysaccharides and the development of polysaccharide antitumor drugs and immune adjuvants provide reference.

The invention discloses a Mycoplasma bovis Mbov_0475 gene mutant strain, which belongs to the technical field of prevention and treatment of animal infectious diseases. The mutant strain is named as Mycoplasma bovis (Mycoplasma bovis) T9.55, and is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2020082, the nucleotide sequence of the Mbov_0475 gene is shown in SEQ ID NO: 1. The strain can induce the proliferation of macrophages and improve the viability of macrophages. Since bovine macrophages are important immune cells for the host to resist pathogenic infection, the mutant strain is expected to play an important role in the field of immune prevention and treatment of Mycoplasma bovis.

The invention relates to application of oligonucleotide YW002 CpG ODN containing a CG sequence motif in preventing and treating periodontitis bone absorption, and belongs to the technical field of molecular medicines. The YW002 CpG ODN can high-efficiently and quickly enter cells and does not need a carrier; on a gene level, a protein level and an osteoblast secretion function level, an osteogenicdifferentiation capacity and a function of osteoblast are remarkably improved by the YW002 CpG ODN; in addition, the YW002 CpG ODN not only inhibits macrophage proliferation and promotes apoptosis, but also remarkably inhibits gene and protein expressions in an osteoclast differentiation process, and influences the maturity of the osteoclast at the same time. The YW002 CpG ODN has a remarkably superior influence on osteogenesis and osteolysis compared with 2006 CpG ODN, and the YW002 CpG ODN can realize double conditions favorable to osteanagenesis for promoting osteogenesis and inhibiting osteolysis.