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Preparation method and application of small-molecule panax notoginseng polysaccharide extract

A technology of notoginseng polysaccharides and small molecules, which is applied in the field of extraction and separation of plant active substances, can solve the problems of low drug activity, low utilization of macromolecular polysaccharides, and difficult absorption, and achieve high pharmacological activity, low starch residue, and industrialization. high degree of effect

Inactive Publication Date: 2017-05-31
YUNNAN YUNKE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no report on the extraction method of the active small-molecule polysaccharides of Panax notoginseng, and the relevant reports mainly focus on the extraction method and pharmacological activity of the total polysaccharides of Panax notoginseng; The total polysaccharides of Panax notoginseng are obtained by precipitation, deproteinization, and drying. However, since the extracted macromolecular polysaccharides contain soluble cellulose, starch, etc., the medicinal activity is very low, and most of the macromolecular polysaccharides have molecular weights of tens of millions or millions. , the human body is difficult to absorb, and the human body needs to be metabolized into small molecular components in order to truly absorb and function, so the utilization of macromolecular polysaccharides is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: The preparation method of the small molecule Panax notoginseng polysaccharide extract is as follows:

[0027] (1) Add 95% ethanol solution of 5 times the mass percentage concentration to the pulverized 1kg Panax notoginseng powder for extraction for 3 hours, filter the filter residue and repeat the extraction twice, discard the filtrate to obtain the medicinal residue;

[0028] (2) Add water 8 times the mass of the dregs to the dregs in step (1) and extract twice at 90-93°C. After 2 hours of extraction, filter to obtain the filtrate and discard the dregs;

[0029] (3) Place the filtrate of step (2) at 40°C and concentrate it in vacuum to a Baume degree of 1.1, then add absolute ethanol so that the ethanol concentration in the mixture is 93%, let it stand for 3 hours, and filter to obtain a precipitate;

[0030] (4) Dissolve the precipitate in step (3) with pure water 12 times its mass, then add trifluoroacetic acid with a mass of 4% of the mixed solution, and...

Embodiment 2

[0035] Embodiment 2: The preparation method of the small molecule Panax notoginseng polysaccharide extract is as follows:

[0036] (1) Add 4 times the mass of absolute ethanol to the pulverized 1kg Panax notoginseng powder for extraction for 2 hours, filter the filter residue and repeat the extraction once, discard the filtrate to obtain the medicinal residue;

[0037] (2) Add water 7 times the mass of the dregs to the dregs of step (1) and extract twice at 90-93°C. After 1.5 hours of extraction time, filter to obtain the filtrate and discard the dregs;

[0038] (3) Place the filtrate of step (2) at 50°C for vacuum concentration to a Baume degree of 1.1, then add absolute ethanol so that the ethanol concentration in the mixture is 97%, let it stand for 4 hours, and filter to obtain a precipitate;

[0039] (4) Add 10 times the mass of the precipitate in step (3) to dissolve it in purified water, then add 2% lysozyme to the mass of the mixture, hydrolyze at 35°C for 5 hours, hea...

Embodiment 3

[0044] Embodiment 3: The preparation method of the small molecule Panax notoginseng polysaccharide extract is as follows:

[0045] (1) Add 4 times the mass of anhydrous ethanol to the pulverized 1kg Panax notoginseng powder for extraction for 2 hours, filter the filter residue and repeat the extraction once, discard the filtrate to obtain the medicinal residue;

[0046] (2) Add water 6 times the mass of the dregs to the dregs in step (1) and extract once at 90-95°C. After an extraction time of 1.5 hours, filter to obtain the filtrate and discard the dregs;

[0047] (3) Place the filtrate of step (2) at 50°C for vacuum concentration to a Baume degree of 1.1, then add absolute ethanol so that the ethanol concentration in the mixture is 97%, let it stand for 4 hours, and filter to obtain a precipitate;

[0048] (4) Dissolve the precipitate in step (3) with 10 times the mass of pure water, then add trifluoroacetic acid with 3% mass of the mixed solution, seal and hydrolyze at 90°C...

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PUM

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Abstract

The invention discloses a preparation method of small-molecule panax notoginseng polysaccharide extract. The preparation method includes: using panax notoginseng as the raw material, crushing the raw material, extracting with an ethanol solution, extracting panax notoginseng residues with water obtain crude panax notoginseng polysaccharide extract, performing ethanol precipitation, dissolving precipitate with water, and then adding trifluoroacetic acid or lysozyme to perform hydrolysis to obtain crude small-molecule polysaccharide liquid; using a cation-anion exchange column to remove the protein, pigment and the like of the crude small-molecule polysaccharide liquid, using activated carbon powder for decoloring, filtering with a titanium-bar filter to obtain a colorless low-viscosity small-molecule polysaccharide solution, concentrating, and performing freeze drying to obtain the small-molecule panax notoginseng polysaccharide extract. The preparation method has the advantages that the method is simple to operate, low in cost and high in industrialization degree; the small-molecule panax notoginseng polysaccharide extract can promote macrophage proliferation, further differentiation and maturation of macrophage can be achieved to obtain activated macrophage, and the phagocytic ability of the macrophage can be evidently enhanced; fast increasing of cell immunity during a cell culture immunity test can be achieved; the extract has application prospect in immunological disease treatment, and the preparation method is suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method and application of a small-molecule Panax notoginseng polysaccharide extract, belonging to the field of extraction and separation of plant active substances. Background technique [0002] Many reports in the study of plant sugar chemistry point out that the highly active polysaccharides with curative and disease-preventive effects are basically small molecular polysaccharides, and the efficacy of macromolecular polysaccharides is relatively weak. The immune system is an extremely complex and important physiological system, in which phagocytes are one of the body's first lines of defense against infection by pathogenic microorganisms, including neutrophils, monocytes, macrophages, etc., which are all innate immunity important component of the system. Among them, macrophages can non-specifically phagocytize and kill pathogenic microorganisms, and at the same time release some inflammatory mediators and cytok...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/02A61P37/04
CPCC08B37/0003A61K31/715A61K36/258
Inventor 张文生曾立品罗金明蔡群虎辛文锋
Owner YUNNAN YUNKE PHARMA
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