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Active peptide feed additive and its preparation method and use

A technology of feed additive and active peptide, which is applied in the field of active peptide feed additive and its preparation, can solve the problems of antigen allergic reaction, high preparation cost, difficulty in popularization and use, and inconspicuous efficacy

Active Publication Date: 2005-02-16
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the large amount of active peptide feed additive prepared in the prior art, the product function is not clear, the efficacy is not obvious, the peptide molecular weight is large and still has antigenicity, it is easy to cause antigen allergic reaction, and the preparation cost is high and it is not easy The disadvantages of popularizing and using in the animal breeding industry, so as to provide a feed with low addition amount and clear function, which can improve the absorption and utilization of nutrients (such as protein, minerals, vitamins, etc.) by animals, and increase the output of milk, meat and eggs. and production capacity, improve the quality and flavor of meat, eggs, and milk, stimulate the development of animal immune organs, improve disease resistance and reduce morbidity, and can significantly improve the water quality of aquatic animal breeding, increase the growth rate and survival rate of aquatic animals, and are widely used Active peptide feed additive for poultry, cattle, sheep, pigs, aquatic products and economic animals, which can be used as a substitute for antibiotics

Method used

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  • Active peptide feed additive and its preparation method and use
  • Active peptide feed additive and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1. Preparation of active peptide feed additive L-1 (liquid)

[0074] The Bacillus subtilis was inserted into the sterilized liquid culture medium and cultured at 25°C for 48 hours to obtain a seed liquid.

[0075] The liquid culture medium is a mixture prepared in the following proportions: peptone 1.3g, glucose 3g, molasses 3g, potato extract 50ml, soluble starch 10g, sucrose 10g, beef extract 2g, yeast extract powder 3.5g, soybean meal powder (60 mesh) 1.5g, growth factor 1ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 20.00mg / ml, CaCl 2 .2H 2 O 2.20mg / ml, ZnSO 4 .7H 2 O 0.81mg / ml, FeSO 4 .7H 2 O 0.44mg / ml, MnSO 4 .H 2 O 0.13mg / ml, CuSO 4 .5H 2 O 0.02mg / ml mixture.

[0076] The fermentation substrate-soy protein powder is pulverized to 60 meshes and put into the fermenter, water and carbon source-corn flour and molasses (weight ratio 1:1) are added in sequence, and the weight of the added water is proportional to the weight of the fermentation substra...

Embodiment 2

[0097] Example 2. Preparation of active peptide feed additive L-2 (liquid)

[0098] The Aspergillus niger was inserted into the sterilized liquid culture medium and cultivated at 35°C for 24 hours to obtain the seed liquid.

[0099] The liquid culture medium is a mixture prepared in the following proportions: peptone 2.5g, glucose 5g, molasses 5g, potato extract 100ml, soluble starch 20g, sucrose 30g, beef extract 3g, yeast extract powder 5g, soybean meal 2g, Growth factor 2ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 30.00mg / ml, CaCl 2 .2H 2 O3.50mg / ml, ZnSO 4 .7H 2 O 1.10mg / ml, FeSO 4 .7H 2 O 0.60mg / ml, MnSO 4 .H 2 O 0.20mg / ml, CuSO 4 .5H 2 O 0.04mg / ml mixture.

[0100] The fermentation substrate-soybean meal is crushed to 120 meshes and put into the fermentor, water and carbon source-corn molasses and white sugar (weight ratio = 3: 2) are added in sequence, and the weight of the added water is determined by the weight of the fermentation substrate. The weight rat...

Embodiment 3

[0101] Example 3. Preparation of active peptide feed additive L-3 (liquid)

[0102] The Saccharomonas viridans was added to the sterilized liquid culture medium and cultured at 27°C for 36 hours to obtain the seed liquid.

[0103] The liquid culture medium is a mixed solution prepared in the following proportions: 1.8g peptone, 4g glucose, 4g molasses, 70ml potato extract, 15g soluble starch, 20g sucrose, 2.5g beef extract, 4g yeast extract powder, soybean meal powder (80 mesh) 1.8g, growth factor 1.5ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 25.00mg / ml, CaCl 2 .2H 2 O 3.00mg / ml, ZnSO 4 .7H 2 O 1.00mg / ml, FeSO 4 .7H 2 O 0.50mg / ml, MnSO 4 .H 2 O0.17mg / ml, CuSO 4 .5H 2 O 0.03mg / ml mixed solution.

[0104] The fermented substrate-bean cake is crushed to 80 mesh and put into the fermenter, and water and carbon source-starch are added in sequence. The weight of the added water and the weight of the protein contained in the fermented substrate are 100:10. The weight rat...

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Abstract

The present invention relates to an active peptide feed addictive, as a mixture of one or more plant protein and animal protein, which is the product of the following steps: fermenting and degrading the mixture through bacillus and the like, controlling the degree of hydrolysis at 20-35%, obtaining a fermentation supernatant after a separation post-processing process, concentrating drying the supernatant or directly adsorbing the supernatant onto the carrier, and then low temperature drying it. Said active peptide feed addictive is capable of obviously depressing development of bacillus coli and salmonella, and can prevention and cure diarrhea caused by bacilli or lienteric diarrhea; can exert its functions at a very low concentration, with a very intense effect; can regulate stomach and intestine bacterium group and promote animals digestion functions; can excite animal immune function, reinforce animal disease resistance against a plurality of epidemic diseases and greatly reduce the morbidity and death rate; can promote animal development and improve the growth velocity; can improve the feed use ratio and reduce production costs. Applications of the microorganism fermentation degradation method provided by the present invention have a low fabricating cost, and the obtained peptides have many kinds and high content, without environment pollutions.

Description

Invention field [0001] The invention relates to an active peptide feed additive, in particular to an active peptide (or oligopeptide) feed additive made from animal and plant proteins and a preparation method thereof. Background technique [0002] Since the 1950s, antibiotics, chemical synthetic drugs, hormones, β-stimulants, heavy metals, sedatives and other growth-promoting health care agents have been widely used in animal feeding and feed, resulting in the following problems: drugs in livestock and aquatic products The residue is serious and directly endangers human health; the quality of livestock and poultry products is degraded, lacking market competitiveness and trust; the increase in drug resistance of bacteria and the increase of drug-resistant strains threaten human safety; damage the ecological environment, etc. Due to the many side effects of antibiotics, scientists have been looking for alternatives to antibiotics for the past two to three decades. At the same time,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/04A23J3/14A23J3/34A23K10/12A23K10/20A23K10/22A23K10/24A23K10/26A23K10/30A23K10/33A23K10/37A23K20/147A23K20/163A23K20/28C12N9/50
CPCY02P60/87
Inventor 张日俊于长青
Owner CHINA AGRI UNIV
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