Active peptide feed additive and its preparation method and use
A technology of feed additive and active peptide, which is applied in the field of active peptide feed additive and its preparation, can solve the problems of antigen allergic reaction, high preparation cost, difficulty in popularization and use, and inconspicuous efficacy
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Embodiment 1
[0073] Example 1. Preparation of active peptide feed additive L-1 (liquid)
[0074] The Bacillus subtilis was inserted into the sterilized liquid culture medium and cultured at 25°C for 48 hours to obtain a seed liquid.
[0075] The liquid culture medium is a mixture prepared in the following proportions: peptone 1.3g, glucose 3g, molasses 3g, potato extract 50ml, soluble starch 10g, sucrose 10g, beef extract 2g, yeast extract powder 3.5g, soybean meal powder (60 mesh) 1.5g, growth factor 1ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 20.00mg / ml, CaCl 2 .2H 2 O 2.20mg / ml, ZnSO 4 .7H 2 O 0.81mg / ml, FeSO 4 .7H 2 O 0.44mg / ml, MnSO 4 .H 2 O 0.13mg / ml, CuSO 4 .5H 2 O 0.02mg / ml mixture.
[0076] The fermentation substrate-soy protein powder is pulverized to 60 meshes and put into the fermenter, water and carbon source-corn flour and molasses (weight ratio 1:1) are added in sequence, and the weight of the added water is proportional to the weight of the fermentation substra...
Embodiment 2
[0097] Example 2. Preparation of active peptide feed additive L-2 (liquid)
[0098] The Aspergillus niger was inserted into the sterilized liquid culture medium and cultivated at 35°C for 24 hours to obtain the seed liquid.
[0099] The liquid culture medium is a mixture prepared in the following proportions: peptone 2.5g, glucose 5g, molasses 5g, potato extract 100ml, soluble starch 20g, sucrose 30g, beef extract 3g, yeast extract powder 5g, soybean meal 2g, Growth factor 2ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 30.00mg / ml, CaCl 2 .2H 2 O3.50mg / ml, ZnSO 4 .7H 2 O 1.10mg / ml, FeSO 4 .7H 2 O 0.60mg / ml, MnSO 4 .H 2 O 0.20mg / ml, CuSO 4 .5H 2 O 0.04mg / ml mixture.
[0100] The fermentation substrate-soybean meal is crushed to 120 meshes and put into the fermentor, water and carbon source-corn molasses and white sugar (weight ratio = 3: 2) are added in sequence, and the weight of the added water is determined by the weight of the fermentation substrate. The weight rat...
Embodiment 3
[0101] Example 3. Preparation of active peptide feed additive L-3 (liquid)
[0102] The Saccharomonas viridans was added to the sterilized liquid culture medium and cultured at 27°C for 36 hours to obtain the seed liquid.
[0103] The liquid culture medium is a mixed solution prepared in the following proportions: 1.8g peptone, 4g glucose, 4g molasses, 70ml potato extract, 15g soluble starch, 20g sucrose, 2.5g beef extract, 4g yeast extract powder, soybean meal powder (80 mesh) 1.8g, growth factor 1.5ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 25.00mg / ml, CaCl 2 .2H 2 O 3.00mg / ml, ZnSO 4 .7H 2 O 1.00mg / ml, FeSO 4 .7H 2 O 0.50mg / ml, MnSO 4 .H 2 O0.17mg / ml, CuSO 4 .5H 2 O 0.03mg / ml mixed solution.
[0104] The fermented substrate-bean cake is crushed to 80 mesh and put into the fermenter, and water and carbon source-starch are added in sequence. The weight of the added water and the weight of the protein contained in the fermented substrate are 100:10. The weight rat...
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