42results about How to "Immunocompetent" patented technology

The invention relates to the processing field of a selenium-rich rice by-product, in particular to a preparation method of rice enzymolysis selenium polypeptides with immune activity. According to the method, selenium-rich rice processing by-product broken rice is used to prepare degreased selenium-rich rice powder by grinding, sieving and degreasing, a supernatant is collected after alkaline extraction and centrifugation by using a sodium hydroxide solution, a dilute hydrochloric acid solution is used for acid precipitation, and after centrifugation, a precipitate is taken for washing and then is frozen-dried. The obtained rice proteins, after enzymolysis by using trypsase, are frozen-dried to obtain the rice enzymolysis polypeptides with the selenium content of 0.3-0.4mg / kg. In a cell immune function experiment, the rice enzymolysis selenium polypeptides have good capacity for promotion of cell proliferation and neutral red phagocytosis of macrophage. The rice enzymolysis selenium polypeptides are natural in nutrition, rich in trace element selenium, and suitable to use as auxiliary food materials, nutrient supplements or health food for enhancing immune functions of bodies, and the preparation method is simple and reliable.

The invention provides a cod polypeptide anti-browning agent and a color protection method for fresh potato slices. The preservative achieves the freshness preservation functions through the synergistic effect generated after different components are mixed, and the potato preservative is prepared from, by weight, 0.1-0.3% of cod polypeptide, 0.8-1.0% of calcium chloride and 1.3-1.5% of chitosan. Cod polypeptide has the functions such as bacteriostatic activity, immunological activity, antihypertensive activity and antioxidant activity and contains certain lysine, methionine and cystine which are lack in plant protein. Nowadays, deep sea fish meal protein is widely applied to the fields of grade nutritional supplements, condiments, functional drinks and the like.

The invention discloses ethanol-free aqueous essence used for preventing and repairing multiple skin injuries. The ethanol-free aqueous essence is characterized by being prepared from the following raw materials in percentage by weight: 0.5-20 of butanediol, 0.5-5 of oat beta-glucan, 0.5-5 of trehalose, 0.05-2 of sodium hyaluronate, 0.05-8 of plant root extract, 0.05-5 of 1,3-propylene glycol, 0.05-2 of allantoin, 0.05-3 of an antibacterial agent, 0.01-2 of a preservative, 0-0.2 of disodium EDTA and the balance of water, wherein the total weight percent of the raw materials is 100%. The obtained essence has multiple functions of activating skin, resisting wrinkles, moisturizing, resisting inflammation and repairing keloid scars.

The invention provides a method for testing non-ethylating CpG content, which comprises following steps: adopting specific methylating enzyme SssI to modify CpG dinucleotide cytosine which is 5-methylcytosine,then using nuclease P1 and bacterial alkaline phosphatase to hydrolyze DNA into single deoxynucleoside, using a reversed-phase high effective liquid phase method to test the content of the 5-methylcytosinein DNA hydrolytic samples which are both modifying and un-modifying, and testing the CpG through differences of the 5-methylcytosine detectable amount between the modifying DNA hydrolytic sample and the un-modifying DNA hydrolytic samples.

The invention discloses a preparation method and application of a tanshinone IIA-loaded water-soluble chitosan / gamma-polyglutamic acid nano-composite. The preparation method comprises the steps of adding gamma-polyglutamic acid and water-soluble chitosan into distilled water respectively according to a ratio to dissolve gamma-polyglutamic acid and water-soluble chitosan; dissolving tanshinone IIAinto absolute ethyl alcohol; then adding an ethanol solution of tanshinone IIA into a gamma-polyglutamic acid aqueous solution, and uniformly mixing; dropwise adding a mixed solution into a chitosan aqueous solution, stirring while adding, and continuing to magnetically stir for 1 hour at the speed of 1,000 rpm after adding; then carrying out rotary evaporation to remove an organic solvent, freezing at -20 DEG C; and carrying out freeze drying to obtain solid powder. The preparation method disclosed by the invention is simple and easy to implement. The prepared water-soluble chitosan / gamma-polyglutamic acid nano-composite of tanshinone IIA can greatly improve the in-vitro and in-vivo drug release performance of tanshinone IIA, so that the bioavailability of tanshinone IIA is improved.

The invention discloses a preparation method of a milk goat mammary gland bioreactor for expressing a GP5-M protein in milk. A gene targeting vector of fusion expression of GP5-M is prepared and thenknocked into a first exon of beta-lactoglobulin of a milk goat to screen target cells; a transgenetic milk goat is prepared by using a somatic nucleus transplantation technology; the mammary gland bioreactor is used for producing milk containing a GP5-M fusion protein; and the pure GP5-M fusion protein is separated from the milk, and an adjuvant is added, so as to prepare a gene engineered subunitvaccine and fulfill the aim of preventing and controlling PRRS.

The present invention relates to a novel Bifidobacterium bifidum MG731 strain and a composition for prophylaxis or therapy of cancer, which comprises same. Specifically, the Bifidobacterium bifidum MG731 strain of the present invention exhibits effects of inhibiting cancer cell proliferation, decreasing cancer cell motility, suppressing angiogenesis, and increasing anticancer immune responses as well as downregulating the expression of inflammatory factors, thereby preventing or treating cancer or inflammatory diseases. In addition, when used in combination with a chemotherapeutic agent or immunotherapeutic agent, the Bifidobacterium bifidum MG731 strain of the present invention exhibits a better anticancer effect. Further, the present invention relates to a novel Lactococcus lactis GEN3033 strain and a composition for prophylaxis or therapy of cancer, which comprises same. Specifically, the Lactococcus lactis GEN3033 strain of the present invention exhibits the effect of directly inhibiting cancer cell proliferation and enhancing immunity, thereby preventing or treating cancer. Particularly, the strain has an excellent anticancer effect by secreting metabolites having inhibitory activity against cancer while being established in the gut. In addition, when used in combination with a chemotherapeutic agent or an immunotherapeutic agent, the Lactococcus lactis GEN3033 of the present invention exhibits a better anticancer effect.

A preparation method and application of a water-soluble chitosan / γ-polyglutamic acid nanocomposite loaded with tanshinone ⅡA, in proportion, respectively adding γ-polyglutamic acid and water-soluble chitosan to distilled water to dissolve, Dissolve tanshinone ⅡA in absolute ethanol, then add the ethanol solution of tanshinone ⅡA into the aqueous solution of γ-polyglutamic acid and mix well, add the mixed solution dropwise into the aqueous solution of chitosan, stir while adding, continue to magnetically Stir for 1 hour at 1000 revolutions per minute. Then the organic solvent was removed by rotary evaporation, frozen at -20°C, and then freeze-dried to obtain a solid powder. The preparation method of the present invention is simple and easy, and the prepared water-soluble chitosan / γ-polyglutamic acid nanocomposite of tanshinone IIA can greatly increase the drug release performance of tanshinone IIA in vivo and in vitro, thereby increasing the bioavailability of tanshinone IIA .