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Preparation method and application of tanshinone IIA-loaded water-soluble chitosan/gamma-polyglutamic acid nano-composite

A technology of water-soluble chitosan and nanocomposite, which is applied in the field of pharmaceutical and pharmacy research, can solve the problems of large administration volume, large amount of auxiliary materials, low drug loading amount, etc., and achieves improved curative effect, simple preparation method, and improved in vivo improvement. The effect of absorption properties

Active Publication Date: 2020-04-07
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these preparations still generally have the problem of low drug loading, which leads to a large volume of administration and a large amount of excipients introduced into the body, which has the risk of causing potential toxicity

Method used

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  • Preparation method and application of tanshinone IIA-loaded water-soluble chitosan/gamma-polyglutamic acid nano-composite
  • Preparation method and application of tanshinone IIA-loaded water-soluble chitosan/gamma-polyglutamic acid nano-composite
  • Preparation method and application of tanshinone IIA-loaded water-soluble chitosan/gamma-polyglutamic acid nano-composite

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Precisely weigh γ-PGA dissolved in ultrapure water to make 0.125 mg / ml γ-PGA aqueous solution, weigh 60mg CS and dissolve it in 10ml ultrapure water, weigh 12mg Tanshinone II A Dissolve in 12ml of absolute ethanol, Tanshinone Ⅱ A Add 12ml of γ-PGA solution with a concentration of 0.125 mg / ml to the ethanol solution, place it on a magnetic stirrer and stir for 10min at a speed of 500rpm / min, then slowly add the mixed solution dropwise to the above CS solution, and continue magnetically stirring for 1h , The speed is 1000rpm / min. After the reaction is completed, the suspension is rotary evaporated until the organic solvent is evaporated to obtain CS-γ-PGA-Tanshinone II A clathrate solution. Jiang CS-γ-PGA-Tanshinone Ⅱ A CS-γ-PGA-tanshinone Ⅱ obtained by freeze-drying the clathrate solution A Clathrate powder.

Embodiment 2

[0033] Precisely weigh γ-PGA dissolved in ultrapure water to make 0.25mg / ml γ-PGA aqueous solution, weigh 40mg CS and dissolve in 10ml ultrapure water, weigh 5mg Tanshinone II A Dissolve in 12ml of absolute ethanol, Tanshinone Ⅱ AAdd 8 ml of γ-PGA solution with a concentration of 0.25 mg / ml to the ethanol solution, place it on a magnetic stirrer and stir for 7 minutes at a speed of 300 rpm / min, then slowly add the mixed solution dropwise to the above CS solution, and continue magnetic stirring for 1.5 h, the speed is 2000rpm / min. After the reaction is completed, the suspension is rotary evaporated until the organic solvent is evaporated to obtain CS-γ-PGA-Tanshinone II A clathrate solution. Jiang CS-γ-PGA-Tanshinone Ⅱ A CS-γ-PGA-tanshinone Ⅱ obtained by freeze-drying the clathrate solution A Clathrate powder.

Embodiment 3

[0035] Precisely weigh γ-PGA dissolved in ultrapure water to make 0.4mg / ml γ-PGA aqueous solution, weigh 80mg CS and dissolve in 10ml ultrapure water, weigh 16mg Tanshinone II A Dissolve in 10ml of absolute ethanol, Tanshinone Ⅱ A Add 10ml of γ-PGA solution with a concentration of 1.6 mg / ml to the ethanol solution, place it on a magnetic stirrer and stir for 20min at a speed of 400rpm / min, then slowly add the mixed solution dropwise to the above CS solution, and continue magnetic stirring for 1h , The speed is 3000rpm / min. After the reaction is completed, the suspension is rotary evaporated until the organic solvent is evaporated to obtain CS-γ-PGA-Tanshinone II A clathrate solution. Jiang CS-γ-PGA-Tanshinone Ⅱ A CS-γ-PGA-tanshinone Ⅱ obtained by freeze-drying the clathrate solution A Clathrate powder.

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Abstract

The invention discloses a preparation method and application of a tanshinone IIA-loaded water-soluble chitosan / gamma-polyglutamic acid nano-composite. The preparation method comprises the steps of adding gamma-polyglutamic acid and water-soluble chitosan into distilled water respectively according to a ratio to dissolve gamma-polyglutamic acid and water-soluble chitosan; dissolving tanshinone IIAinto absolute ethyl alcohol; then adding an ethanol solution of tanshinone IIA into a gamma-polyglutamic acid aqueous solution, and uniformly mixing; dropwise adding a mixed solution into a chitosan aqueous solution, stirring while adding, and continuing to magnetically stir for 1 hour at the speed of 1,000 rpm after adding; then carrying out rotary evaporation to remove an organic solvent, freezing at -20 DEG C; and carrying out freeze drying to obtain solid powder. The preparation method disclosed by the invention is simple and easy to implement. The prepared water-soluble chitosan / gamma-polyglutamic acid nano-composite of tanshinone IIA can greatly improve the in-vitro and in-vivo drug release performance of tanshinone IIA, so that the bioavailability of tanshinone IIA is improved.

Description

technical field [0001] The invention belongs to the field of pharmaceutical pharmacy research, and in particular relates to a preparation method and application of a water-soluble chitosan / γ-polyglutamic acid nanocomposite loaded with tanshinone IIA. Background technique [0002] According to the statistics of the World Health Organization, the morbidity and mortality of cardiovascular and cerebrovascular diseases have leapt to the top of all kinds of diseases. According to statistics from the Ministry of Health, the morbidity and mortality of cardiovascular and cerebrovascular diseases in my country rank first among various diseases, and the prevalence and mortality of cardiovascular diseases are still on the rise. It is estimated that the number of patients with cardiovascular diseases in my country is 290 million. So far, there is no effective drug for treatment. The main reason is that the cells that make up the blood brain barrier (BBB) ​​are extremely tightly connected...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K47/36A61K47/34A61K31/58A61P9/00A61P9/10
CPCA61K9/19A61K31/58A61K47/34A61K47/36A61P9/00A61P9/10
Inventor 于洁吴妮郑晓晖张亚军
Owner NORTHWEST UNIV(CN)
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