Method for simultaneously extracting polysaccharide and polyphenol from needle mushrooms
A technology of Flammulina velutipes and polysaccharides, applied to allergic diseases, antineoplastic drugs, medical raw materials derived from fungi, etc., can solve the problems of waste, low extraction rate, and inability to prepare polyphenols at the same time, and achieve high yield and product purity The effect of high and strong antioxidant activity
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Embodiment 1
[0030] A method for simultaneously extracting polysaccharides and polyphenols from Flammulina velutipes, such as figure 1 shown, including the following steps:
[0031] Step (1): after superfine grinding the dry powder of Flammulina velutipes, mix the dry powder of Flammulina velutipes with 0.5% sodium hydroxide solution according to the mass ratio of 1:20;
[0032] Step (2): ultrasonically treat the mixed solution in step (1), the treatment condition is 200Hz, 800W, 10 minutes, the treatment solution is centrifuged at 6000 rpm for 10 minutes, and the supernatant I and precipitate are separated;
[0033] Step (3): Add deionized water 10 times the weight of the precipitate to the precipitate in step (2), mix well, adjust the pH value to 4, add 0.1% cellulase by weight of the mixture, and set the temperature at 45°C Enzymolysis for 20 minutes, centrifugation at 6000 rpm for 10 minutes to separate supernatant II and precipitate;
[0034] Step (4): Combine the supernatant I in s...
Embodiment 2
[0037] A method for simultaneously extracting polysaccharides and polyphenols from Flammulina velutipes, such as figure 1 shown, including the following steps:
[0038] Step (1): After superfine grinding the dry powder of Flammulina velutipes, mix the dry powder of Flammulina velutipes with 1% sodium hydroxide solution at a mass ratio of 1:30;
[0039] Step (2): ultrasonically treat the mixed solution in step (1), the treatment condition is 400Hz, 200W, 20 minutes, the treatment solution is 6000 rpm / centrifuge for 10 minutes, and the supernatant I and precipitate are separated;
[0040] Step (3): Add deionized water 8 times the weight of the precipitate to the precipitate in step (2), mix well, adjust the pH value to 4.5, add 0.3% cellulase by weight of the mixture, and set the temperature at 50°C Enzymolysis for 30 minutes, centrifugation at 6000 rpm for 10 minutes to separate supernatant II and precipitate;
[0041] Step (4): Combine the supernatant I in step (2) and the s...
Embodiment 3
[0044] A method for simultaneously extracting polysaccharides and polyphenols from Flammulina velutipes, such as figure 1 shown, including the following steps:
[0045] Step (1): after superfine grinding the dry powder of Flammulina velutipes, mix the dry powder of Flammulina velutipes with 1.5% sodium hydroxide solution in a mass ratio of 1:25;
[0046]Step (2): Ultrasonic treatment of the mixed solution in step (1), the treatment conditions are 100Hz, 1000W, 25 minutes, the treatment solution is 6000 rpm / centrifugation for 10 minutes, and the supernatant I and precipitate are separated;
[0047] Step (3): Add deionized water 12 times the weight of the precipitate to the precipitate in step (2), mix well, adjust the pH to 5, add 0.4% cellulase by weight of the mixture, and set the temperature at 50°C Enzymolysis for 40 minutes, centrifugation at 6000 rpm for 10 minutes to separate supernatant II and precipitate;
[0048] Step (4): Combine the supernatant I in step (2) and t...
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