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Method for simultaneously extracting polysaccharide and polyphenol from needle mushrooms

A technology of Flammulina velutipes and polysaccharides, applied to allergic diseases, antineoplastic drugs, medical raw materials derived from fungi, etc., can solve the problems of waste, low extraction rate, and inability to prepare polyphenols at the same time, and achieve high yield and product purity The effect of high and strong antioxidant activity

Active Publication Date: 2014-05-14
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the defects such as low extraction rate and inability to simultaneously prepare polyphenol components resulting in waste when extracting polysaccharides from Flammulina velutipes in the prior art, and provides a method for simultaneously extracting polysaccharides and polyphenols from edible and medicinal mushrooms Flammulina velutipes

Method used

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  • Method for simultaneously extracting polysaccharide and polyphenol from needle mushrooms

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Effect test

Embodiment 1

[0030] A method for simultaneously extracting polysaccharides and polyphenols from Flammulina velutipes, such as figure 1 shown, including the following steps:

[0031] Step (1): after superfine grinding the dry powder of Flammulina velutipes, mix the dry powder of Flammulina velutipes with 0.5% sodium hydroxide solution according to the mass ratio of 1:20;

[0032] Step (2): ultrasonically treat the mixed solution in step (1), the treatment condition is 200Hz, 800W, 10 minutes, the treatment solution is centrifuged at 6000 rpm for 10 minutes, and the supernatant I and precipitate are separated;

[0033] Step (3): Add deionized water 10 times the weight of the precipitate to the precipitate in step (2), mix well, adjust the pH value to 4, add 0.1% cellulase by weight of the mixture, and set the temperature at 45°C Enzymolysis for 20 minutes, centrifugation at 6000 rpm for 10 minutes to separate supernatant II and precipitate;

[0034] Step (4): Combine the supernatant I in s...

Embodiment 2

[0037] A method for simultaneously extracting polysaccharides and polyphenols from Flammulina velutipes, such as figure 1 shown, including the following steps:

[0038] Step (1): After superfine grinding the dry powder of Flammulina velutipes, mix the dry powder of Flammulina velutipes with 1% sodium hydroxide solution at a mass ratio of 1:30;

[0039] Step (2): ultrasonically treat the mixed solution in step (1), the treatment condition is 400Hz, 200W, 20 minutes, the treatment solution is 6000 rpm / centrifuge for 10 minutes, and the supernatant I and precipitate are separated;

[0040] Step (3): Add deionized water 8 times the weight of the precipitate to the precipitate in step (2), mix well, adjust the pH value to 4.5, add 0.3% cellulase by weight of the mixture, and set the temperature at 50°C Enzymolysis for 30 minutes, centrifugation at 6000 rpm for 10 minutes to separate supernatant II and precipitate;

[0041] Step (4): Combine the supernatant I in step (2) and the s...

Embodiment 3

[0044] A method for simultaneously extracting polysaccharides and polyphenols from Flammulina velutipes, such as figure 1 shown, including the following steps:

[0045] Step (1): after superfine grinding the dry powder of Flammulina velutipes, mix the dry powder of Flammulina velutipes with 1.5% sodium hydroxide solution in a mass ratio of 1:25;

[0046]Step (2): Ultrasonic treatment of the mixed solution in step (1), the treatment conditions are 100Hz, 1000W, 25 minutes, the treatment solution is 6000 rpm / centrifugation for 10 minutes, and the supernatant I and precipitate are separated;

[0047] Step (3): Add deionized water 12 times the weight of the precipitate to the precipitate in step (2), mix well, adjust the pH to 5, add 0.4% cellulase by weight of the mixture, and set the temperature at 50°C Enzymolysis for 40 minutes, centrifugation at 6000 rpm for 10 minutes to separate supernatant II and precipitate;

[0048] Step (4): Combine the supernatant I in step (2) and t...

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Abstract

The invention relates to the technical field of extraction of active ingredients of edible mushrooms, and particularly relates to a method for simultaneously extracting polysaccharide and polyphenol from needle mushrooms. The method comprises the steps: preparing an extraction solution, extracting an alkaline solution by assistance of ultrasonic wave, performing enzymolysis on residue, extracting polyphenol from ethyl acetate and extracting polysaccharide after ultrafiltration and desalination. According to the method, operations such as auxiliary extraction, enzymolysis and membrane filtration are organically combined, and the polysaccharide and the polyphenol are simultaneously extracted, so that the production process is simplified, the safety and reliability are achieved, the yield is high, the physiological and pharmacological activities of the polysaccharide and the polyphenol in a needle mushroom sporocarp are well reserved, and a larger economic value is achieved.

Description

technical field [0001] The invention relates to the technical field of extracting effective components of edible fungi, in particular to a method for simultaneously extracting polysaccharides and polyphenols from Flammulina velutipes. Background technique [0002] Flammulina velutipes (Flammulina velutipes) has another name called mushroom, winter mushroom, belongs to fungi door, Basidiomycetes, Agaricaceae, Tricholomaceae, Chrysanthemum genus. Flammulina velutipes is extremely nutritious, and has the laudatory titles such as Zengzhi mushroom and "Yixiu mushroom". It is a famous edible fungus in the world and has developed into the third largest edible fungus at present. Flammulina velutipes is tender and refreshing, delicious and fragrant, and rich in nutrition. It is a very high-end delicacy with high nutritional value. It is reported in the literature that Flammulina velutipes contains higher protein, carbohydrates and crude fiber, but lower fat content, so it is a rare ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K36/06A61P39/06A61P37/02A61P35/00
Inventor 张作法范雷法吕国英苗立祥潘慧娟
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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