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Temperature-sensitive hydrogel having bioactivity and preparation method of temperature-sensitive hydrogel

A temperature-sensitive hydrogel and biologically active technology, applied in medical science, prostheses, etc., can solve the problems of unfavorable nerve cell selective adsorption and activation of specific genes, lack of biological activity, etc.

Active Publication Date: 2015-09-02
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the biggest disadvantage of artificially synthesized polymer hydrogels is the lack of biological activity, which is not conducive to the selective adsorption of nerve cells and the activation of some specific genes. Therefore, it is necessary to modify the synthetic polymer hydrogels with biological activity. The biomimetic design of the synthetic polymer hydrogel uses the multi-component composite technology to introduce some biologically active molecules into the surface of the synthetic polymer hydrogel, in order to change the selective adsorption of the synthetic polymer hydrogel to nerve cells and promote nerve axis. Burst growth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for preparing a thermosensitive hydrogel with biological activity, the steps are as follows:

[0035] 1) Preparation of HO-PEG-COOH: Dissolve 500g PEG (Mn=2000) in 50L dry tetrahydrofuran, add 8.9025g CaH 2 , stirred at room temperature for 1 hour, then added 47.3 mL of ethyl bromoacetate, stirred at room temperature for 2 hours, filtered, added n-hexane to the filtrate to precipitate the PEG mixture, filtered the resulting precipitate, and vacuum-dried the precipitate at room temperature After 12 hours, a PEG mixture was obtained. Prepare the PEG mixture into a 0.2 g / mL aqueous solution, add 425 mL of 1 mol / L NaOH aqueous solution, stir for 2 hours, adjust the pH value of the system to 12.5 with 1 mol / L hydrochloric acid solution, add dichloromethane for initial extraction, take the aqueous phase, drop Add 1mol / L HCl solution to adjust the pH value to 3, add dichloromethane again for secondary extraction, take the organic phase, add n-hexane, filter to obtain...

Embodiment 2

[0043] A method for preparing a thermosensitive hydrogel with biological activity, the steps are as follows:

[0044] 1) Preparation of HO-PEG-COOH: Dissolve 500g PEG (Mn=2000) in 7L dry THF, add 8.9025g CaH 2 , stirred at room temperature for 1 hour, then added 47.3 mL of ethyl bromoacetate, stirred at room temperature for 2 hours, filtered, added n-hexane to the filtrate to precipitate the PEG mixture, filtered the resulting precipitate, and vacuum-dried the precipitate at room temperature For 48 hours, a PEG mixture was obtained. Prepare the PEG mixture into a 0.2 g / mL aqueous solution, add 425 mL of 1 mol / L NaOH aqueous solution, stir for 2 hours, adjust the pH value of the system to 12.5 with 1 mol / L hydrochloric acid solution, add dichloromethane for initial extraction, take the aqueous phase, drop Add 1mol / L HCl solution to adjust the pH value to 3, add dichloromethane again for secondary extraction, take the organic phase, add n-hexane, filter to obtain HO-PEG-COOH; ...

Embodiment 3

[0052] A method for preparing a thermosensitive hydrogel with biological activity, the steps are as follows:

[0053] 1) Preparation of HO-PEG-COOH: Dissolve 500g PEG (Mn=2000) in 10L dry THF, add 8.9025g CaH 2 , stirred at room temperature for 1 hour, then added 47.3 mL of ethyl bromoacetate, stirred at room temperature for 2 hours, filtered, added n-hexane to the filtrate to precipitate the PEG mixture, filtered the resulting precipitate, and vacuum-dried the precipitate at room temperature For 48 hours, a PEG mixture was obtained. Prepare the PEG mixture into a 0.6 g / mL aqueous solution, add 425 mL of 1 mol / L NaOH aqueous solution, stir for 2 hours and then adjust the pH value of the system to 12.5 with 1 mol / L hydrochloric acid solution, add dichloromethane for primary extraction, take the aqueous phase, Add 1mol / L HCl solution dropwise to adjust the pH value to 3, add dichloromethane again for secondary extraction, take the organic phase, add n-hexane, filter to obtain H...

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Abstract

The invention relates to a temperature-sensitive hydrogel having bioactivity and a preparation method of the temperature-sensitive hydrogel. The preparation method comprises the following steps: 1) preparing HO-PEG-COOH; 2) preparing Ac-PEG-COOH; 3) preparing Ac-PEG-Ac; 4) preparing Ac-PEG-g-IKVAV; 5) preparing a PNIPAM-b-PEG-g-IKVAV precursor; and 6) preparing the temperature-sensitive hydrogel having the bioactivity, namely dissolving the PNIPAM-b-PEG-g-IKVAV precursor into DMF, and then adding PNIPAM, a micromolecular cross-linking agent, Ac-PEG-Ac and AIBN, sealing and vacuumizing and then reacting at 70 DEG C for 12 hours. The preparation method is simple in process; the obtained hydrogel is quick in response to temperature change response to temperature change, has bioactivity and is capable of promoting the adhesion and proliferation of nerve cells; as a result, a support materials is provided for central nervous restoration.

Description

technical field [0001] The invention relates to a temperature-sensitive hydrogel with biological activity and a preparation method thereof. Background technique [0002] For a long time, the repair of peripheral nerve injuries is still not ideal clinically, and there is nothing to do about thick, long-segment nerve defects and multiple nerve injuries. People have been exploring and looking for bridges that can effectively replace autologous nerves. With the development of tissue engineering and neuroscience, the principles of tissue engineering are used to prepare nerve tissue grafts to replace autologous nerves and bridge long-segment damage. Nerves have achieved good curative effect, so neural tissue engineering grafts have received more and more attention, and tissue engineering is expected to solve the problem of nerve tissue repair. In recent years, people have gradually realized that matrix materials play an important role in tissue engineering. With the deepening of ...

Claims

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Application Information

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IPC IPC(8): C08F291/00C08F291/12C08F291/06C08F290/06C08F222/38C08F299/02C08F120/54C08G65/48A61L27/54A61L27/52A61L27/18A61L27/22A61L27/16
Inventor 戴红莲杨冬张平
Owner WUHAN UNIV OF TECH
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