48 results about "Neural tissue engineering" patented technology

The invention relates to a preparation method for a conduction and sustained release type nervous tissue engineering scaffold. The method comprises the following steps: silk fibroin Silk and lactic acid-caprolactone copolymer are dissolved in a solvent, dissolved and stirred to obtain a solution, then polyaniline and camphorsulfonic acid are added and stirred and mixed uniformly to obtain a cortex electrospinning solution, and NGF (nerve growth factors) are dissolved in ultrapure water completely to obtain a core electrospinning solution; and the cortex electrospinning solution and the core electrospinning solution are accommodated in an injector respectively for coaxial electrospinning, and then fumigation treatment and vacuum drying are performed to obtain the conduction type nervous tissue engineering scaffold. According to the prepared nanofiber scaffold, the nerve repair speed is increased from ways including external electrical stimulation (conductive polymer), biochemistry (NGFs), a topological structure required by nerve regeneration (orientation guide) and the like. The method is simple to operate, good in repeatability and high in economic benefit, and provides novel experimental thought for nerve defect repair in clinical application.

The invention provides a neutral restoration catheter support of a multilayer structure and manufacturing method of the neutral restoration catheter support in order to meet requirements of neural tissue engineering. The neutral restoration catheter support structurally comprises an inner pipe, a middle pipe and an outer pipe which are made of incompletely different materials, the inner pipe is made of poly-dl-lactide, the middle pipe is made of a lactic acid caprolactone copolymer, and the outer pipe is made of poly-l-lactide. The neutral catheter support is characterized in that the support has certain mechanical performance, is bendable and extrudable, is 10-15MPa in tensile strength and 5-13% of tensile deformation and can restore to an original form after being bent or extruded.

The invention relates to a conductive silk fibroin material and a preparation method thereof. The preparation method mainly comprises the following steps: (1) insoluble treatment of silk fibroin material; (2) 2-((2,3-dihydrothieno[3,4-b][1,4]dioxene-2-yl)methoxy)acetic acid activation product graft modification of silk fibroin material subjected to insoluble treatment; and (3) in-situ oxidation polymerization of thieno[3,4-b]-1,4-dioxin-2-methanol on silk fibroin material surface subjected to graft modification. The method can be used for preparing the silk fibroin conductive composite material with the polythieno[3,4-b]-1,4-dioxin-2-methanol grafted on the surface; the surface sheet resistance is 0.9*10<5>-5*10<7>ohm; the preparation technique is simple and mild; and the obtained novel biological material has high application value, and especially can be used as a nervous tissue engineering material and a neural restoration material.

The invention discloses an oriented conductive collagen hydrogel, a biomimetic conductive nerve scaffold material and a preparation method of the oriented conductive collagen hydrogel and the biomimetic conductive nerve scaffold material. The mixture of natural biological macromolecular collagen and polymer nanoparticles with excellent biocompability is used as a raw material, and oriented conductive collagen hydrogel fiber is prepared by a coaxial microfluidic chip with a PEG (polyethylene glycol) buffer solution; the biomimetic conductive nerve scaffold material with in situ loading of cellsis obtained by adding the cells in the process of hydrogel preparation. The hydrogel fiber prepared has the advantages of electrical conductivity matching with natural nerve tissue, similar mechanical properties, good biocompatibility, and can be arranged along the direction of the hydrogel fiber on a micro and nano scale, and can simulate the directional structure in the natural nerve tissue; the biomimetic conductive nerve scaffold material prepared can stimulate the arrangement of neurous in the nerve tissue along the direction of the nerve fiber and conduction of electrical signals alongaxons, and shows a good application prospect in the field of neural tissue engineering.

The invention discloses preparation and application of a linear biodegradable polyester elastomer with a controllable elasticity and shape memory effect. The linear biodegradable polyester elastomer with the controllable elasticity and shape memory effect is formed by conducting copolymerization on a caprolactone monomer containing a lateral cyclic ether structural unit and caprolactone, wherein the molar ratio of the caprolactone monomer containing the lateral cyclic ether structural unit to the caprolactone is 5:95-25:75, and the structural formula can be found in description. The viscoelasticity of the linear biodegradable polyester elastomer with the controllable elasticity and shape memory effect is remarkably improved, the elongation at break can reach 1600% and above, the elastomer can be dissolved in a conventional organic solvent, a three-dimensional porous support can be constructed conveniently through a construction technology of the porous support such as, electrostatic spinning, three-dimensional printing and phase separation, tissue engineering blood vessel scaffold materials, myocardial patches, nervous tissue engineering scaffold materials and the like are prepared, and the preparation and application of the linear biodegradable polyester elastomer can be widely applied to the fields of soft tissue engineering scaffolds, tissue repair and regenerative medicine.

A degradable tubular high-molecular porous foam material used as the porous cell scaffold of blood vessel tissue or nerve tissue engineering is prepared from a high-molecular material through use of combined mould. Its advantages are high porosity up to 90% and uniform distribution of pores.

The invention discloses a method for directionally differentiating human iPSCs (induced pluripotent stem cells)-derived 3D (three-dimensional) retinas by means of induction to obtain retinal ganglion cells. The method includes digesting nerve fiber layers of the human iPSc-derived 3D retinas to obtain single cells; inoculating seed cells, namely the single cells, in induction differentiation culture media and cultivating the seed cells in the induction differentiation culture media; differentiating the seed cells to obtain the retinal ganglion cells. The induction differentiation culture media are culture media with neurotrophic factors and concentration-maintained retinal differentiation nutrient liquid. The method has the advantages that a novel functional RGCs (retinal ganglion cells) research approach is obtained from an angle of stem cell induction differentiation, a novel research idea is provided for directionally differentiating human iPSc-RGCs, a novel research clue is provided for investigating human iPSc-RGCs regulation mechanisms, a novel seed cell is provided for research on nerve tissue engineering, and theoretical and laboratory bases are laid for treating visual impairment by means of stem cell transplantation.

The invention discloses a three-dimensional active complex of dental pulp stem cells for nervous tissue engineering and a three-dimensional construction method thereof. The three-dimensional active complex and the three-dimensional construction method disclosed by the invention have the main beneficial effects that a GelMA-bFGF hydrogel complex is constructed by GelMA hydrogel carrying bFGF, and as an active carrier for three-dimensional culture of the dental pulp stem cells, the three-dimensional active complex can provide a supporting effect, an exchange effect and active microenvironment and other effects for culturing the dental pulp stem cells; a large amount of active dental pulp stem cells can be effectively obtained, the benefit for proliferation and fast growth of the dental pulp stem cells is achieved, and the active three-dimensional-culture dental pulp stem cells can be effectively obtained for application in the nervous-tissue engineering research.

The invention discloses a PCL (polycaprolactone)/CNTs (carbon nanotubes) compound electrospun film with optimal fiber orientation for inducing nerve regeneration. The PCL/CNTs compound electrospun film has the characteristics of large specific surface area and high porosity, is very similar to an extracellular matrix, and can be used as a support for supporting regeneration of various cells into complete tissues. The introduction of CNTs can further enhance the electrical activity of the composite support, so that the repair efficiency of nervous tissues is further improved under the action ofelectrical stimulation. The obtained PCL/CNTs compound electrospun film with the optimal fiber orientation lays a solid theoretical foundation for nerve defect repair in nervous tissue engineering, the process is simple, convenient, and easy to operate, and popularization and application are facilitated.

The invention discloses a choline polysaccharide conductive nervous tissue engineering hydrogel material as well as a preparation method and the application thereof, and relates to conductive nervous tissue engineering polysaccharide hydrogel and a preparation method thereof. The invention aims to solve the problem that the existing conductive nervous tissue engineering hydrogel material cannot give consideration to excellent conductivity and biological signal transduction functions, so that the conductive nervous tissue engineering hydrogel material is poor in biological signal transduction capability. The preparation method comprises the following steps: 1, preparing carboxymethyl chitosan; 2, preparing catechol carboxymethyl chitosan; 3, preparing choline-catechol carboxymethyl chitosan; and 4, preparing the choline polysaccharide conductive nerve tissue engineering hydrogel material. The choline polysaccharide conductive nervous tissue engineering hydrogel material is used as a nervous tissue engineering material for promoting defective nerve repair. The choline polysaccharide conductive nerve tissue engineering hydrogel material can be obtained.

The invention discloses a keratin fiber-nerve growth factor (NGF) composite stent of nervous tissue engineering and a preparation method thereof. The preparation method for the keratin fiber-nerve growth factor composite stent of the nervous tissue engineering comprises the following steps: processing keratin fibers by using acidic solution, breaking an epidermal layer on the topmost surface, andexposing an internal cortical layer, to obtain the processed keratin fibers; enabling a nerve growth factor to be loaded on the processed keratin fibers, to obtain the keratin fiber-nerve growth factor composite stent. The provided composite stent can be used for remarkably promoting the survival of neural stem cells and increasing a proportion of the neural stem cells differentiated as nerve cells and an expression number of acceptors Trk A. So the NGF-keratin fiber can be used as a novel three-dimensional stent. The composite stent is capable of providing a new method and idea for treating nervous system injuries or diseases by using a nervous tissue engineering method.

The invention relates to a method for constructing three-dimensional engineered nervous tissue, the three-dimensional engineered nervous tissue and an application thereof. Process. The method comprises the following steps: 1, carrying out isolated culture on animal neural stem cells; 2, applying a physiological electric field; subculturing the neural stem cells isolated and cultured in the step 1to the fifth generation, centrifuging neural spheres with a diameter of 100-200 microns for later use, pre-melting matrix gel Matrigel on ice for later use, and continuously applying a physiological electric field to stimulate for 7-14 days through physiological electric field application equipment for 1-2 hours every day; 3, optimizing a culture solution suitable for engineering nerve tissue growth: adding 1/50 of B27, 1/100 of N2, 10-20 ng/ml of bFGF and 1% of fetal calf serum into DMEM/F12; and 4, applying the physiological electric field for 7-14 days to obtain the three-dimensional engineered nervous tissue. The technical problems that in the application process of neural tissue engineering, the number of neuronal differentiation of existing neural stem cells is extremely small, and neurite cannot be induced to grow orderly in a three-dimensional growth environment to form a developed neural network are solved.

The invention belongs to the technical field of medical material preparation, and provides a preparation method of a degradable piezoelectric fiber stent and a product thereof. The preparation method of the degradable piezoelectric fiber stent comprises the following steps: preparing a gelatin solution, performing electrostatic spinning to prepare a fiber membrane, and drying to obtain a gelatin fiber membrane; placing the gelatin fiber membrane in glutaraldehyde saturated steam for normal-temperature crosslinking, and drying to obtain the degradable piezoelectric fiber stent. The preparation method provided by the invention adopts electrostatic spinning and chemical crosslinking modes for preparation, the method is simple, and reaction conditions are easy to realize. The prepared degradable piezoelectric fiber membrane stent can induce neural differentiation of neural stem cells in a wireless stimulation mode, and has important significance in clinical and nervous tissue engineering.