48 results about "Neural tissue engineering" patented technology

Disclosed is a nervous tissue engineering tubular supporting stand and method for making same, wherein the nervous tissue engineering tubular supporting stand comprises a chitosan pipe wall and biological source filling base material with axial multiple passages, and the method consists of preparing semipermeable chitosan hollow pipe with 1-5mm of inside diameter, pouring large biological molecule solvent such as chitosan, collagen or gelatine, and utilizing special-purpose die arrangement and lyophilization technology. The obtained multiple-pass nerve channel with bionic construction is beneficial for cell adhesion, migration and leading of neuraxon directional growth, and suitable of the renovation and regeneration of nerve damages.

The invention relates to a preparation method for a conduction and sustained release type nervous tissue engineering scaffold. The method comprises the following steps: silk fibroin Silk and lactic acid-caprolactone copolymer are dissolved in a solvent, dissolved and stirred to obtain a solution, then polyaniline and camphorsulfonic acid are added and stirred and mixed uniformly to obtain a cortex electrospinning solution, and NGF (nerve growth factors) are dissolved in ultrapure water completely to obtain a core electrospinning solution; and the cortex electrospinning solution and the core electrospinning solution are accommodated in an injector respectively for coaxial electrospinning, and then fumigation treatment and vacuum drying are performed to obtain the conduction type nervous tissue engineering scaffold. According to the prepared nanofiber scaffold, the nerve repair speed is increased from ways including external electrical stimulation (conductive polymer), biochemistry (NGFs), a topological structure required by nerve regeneration (orientation guide) and the like. The method is simple to operate, good in repeatability and high in economic benefit, and provides novel experimental thought for nerve defect repair in clinical application.

The invention discloses a preparation method of an electroactive micron / nanometer fiber scaffold which takes a degradable polymer as a shell and a conductive polymer as a core, has a core-shell structure and is formed by interlacing of composite fibers. The preparation method comprises the steps of preparing core spraying liquid; preparing shell spinning liquid; preparing conductive high-polymer / degradable polymer composite fibers with an electroactive core-shell structure and a composite fiber dual-pore structure scaffold by using a coaxial co-spinning device and combining a spinning technology with a spraying technology; cleaning and drying to obtain the scaffold. The electroactive micron / nanometer fiber scaffold prepared by the invention has stable conductivity and good biocompatibility required by electro-stimulation on cells, and well avoids direct contact between the conductive high-polymer and a cell culture solution or an organism at the same time, so that the problem of instable conductivity caused by de-doping is solved, and the scaffold has a very good practical value in the aspect of nerve tissue engineering.

The present invention relates to scaffolds that can physically guide cells, e.g. neurons, while best matching the material properties of native tissue. The present invention also relates to methods of generating such scaffolds, and for the use of such scaffolds, e.g. in spinal cord and peripheral nerve injury repair. The methods of the present invention include a uniquely controlled freeze casting process to generate highly porous, linearly oriented scaffolds. The scaffolds of the present invention not only comprise a highly aligned porosity, but also contain secondary guidance structures in the form of ridges running parallel to the pores to create a series of microstructured and highly aligned channels. This hierarchy of structural guidance aligns and guides neurite outgrowth down the channels created by the ridges, and keep neurites from branching perpendicular to the inter-ridge grooves.

The present invention relates to a nerve tissue engineering electric conduction nano-fiber tubular stent and a preparation method thereof. The nerve tissue engineering electric conduction nano-fiber tubular stent comprises an electric conduction nano-fiber tube and an electric conduction hydrogel filling material, wherein the electric conduction nano-fiber tube is formed by compounding an inner layer and an outer layer, the inner layer is an electric conduction nano-fiber film arranged in a parallel manner and oriented along the axial direction of the catheter, the outer layer is a non-electric conduction non-woven arrangement nano-fiber film, and the filling material is formed by compounding a natural macromolecular hydrogel and growth factor-loading electric conduction nano-tubes. According to the present invention, the catheter stent material can provide the multifunctional bionic microenvironment for nerve tissue engineering, has good biocompatibility, and can provide nutrients for nerve repair, wherein the two-dimensionally oriented fiber morphology can promote the differentiation and growth of nerve cells, and with the adding of the electric conduction material, the differentiation degree of nerve cells can be further improved, the cell proliferation can be promoted, and the conditions required by nerve regeneration can be well met.

The invention belongs to the fields of biomedical materials and nerve tissue engineering, and relates to a preparation method of a composite nano-fiber nerve scaffold suitable for peripheral nerve regeneration. The composite nano-fiber scaffold with conductivity and a function of promoting regeneration by traditional Chinese medicines is prepared by taking a biochemical macromolecular / conductive material with biocompatibility as shell pre-electrospinning liquid, an extracellular matrix component or an extracellular matrix component / traditional Chinese medicine extract as a core pre-electrospinning liquid and by an electrostatic spinning technology. The composite nano-fiber scaffold with electric stimulation and traditional Chinese medicines, prepared according to the method, can control effective release of various medicines, is high in biocompatibility, stable in performance and easy to store, and has good application prospect in the fields of biomedicine such as peripheral nerve damage repair. The method is simple in operation, the process is time-saving, the raw materials are moderate in price and easily available, the reaction condition is mild, and the method is environmentally friendly, high in economic performance and suitable for industrial production.

The invention discloses a nervous tissue engineering fibrous membrane and a preparation method. The method comprises the following steps of: dissolving a high molecular material into water or an organic solvent to prepare electrostatic spinning solution; injecting the high molecular solution into an injector; performing electro-spinning to form nanofibers, which are arranged tidily, to prepare a high molecular fibrous membrane; and immersing the high molecular fibrous membrane into pyrrole aqueous solution to form core-sheath fibers. In the preparation method of the nervous tissue engineering fibrous membrane provided by the invention, the fibrous membrane is constituted by conductive core-shell nanofibers; the core is high molecule which has excellent material biocompatibility; the nanofibers are arranged tidily by controlling the preparation conditions; the shell-layer material is polypyrrole; the biocompatibility is good; the prepared core-sheath fiber can provide a unique platform to grow and extend neurites; and the process is simple, easy to operate and low in cost and has broad application prospect in the field of nervous tissue engineering.

The invention provides a method for preparing a chitosan three-dimensional scaffold having graphene oxide coatings of different reducing degrees. The method specifically comprises the following steps: preparing a chitosan scaffold material having a porous structure by a freeze drying method; then soaking the chitosan scaffold material in graphene oxide dispersion fluids of different concentrations; adsorbing graphene oxide sheets to pore walls of chitosan through the electrostatic interaction and the hydrogen-bond interaction; drying, and finally carrying out gradient heating and reducing to obtain the chitosan scaffold which has greatly improved mechanical strength and has graphene oxide coatings of different reducing degrees. The chitosan scaffold has a good application prospect in the aspect of neural tissue engineering. The method provided by the invention is simple to operate and can be used for preparing a great amount of desired product.

The invention provides a neutral restoration catheter support of a multilayer structure and manufacturing method of the neutral restoration catheter support in order to meet requirements of neural tissue engineering. The neutral restoration catheter support structurally comprises an inner pipe, a middle pipe and an outer pipe which are made of incompletely different materials, the inner pipe is made of poly-dl-lactide, the middle pipe is made of a lactic acid caprolactone copolymer, and the outer pipe is made of poly-l-lactide. The neutral catheter support is characterized in that the support has certain mechanical performance, is bendable and extrudable, is 10-15MPa in tensile strength and 5-13% of tensile deformation and can restore to an original form after being bent or extruded.

The invention relates to a conductive silk fibroin material and a preparation method thereof. The preparation method mainly comprises the following steps: (1) insoluble treatment of silk fibroin material; (2) 2-((2,3-dihydrothieno[3,4-b][1,4]dioxene-2-yl)methoxy)acetic acid activation product graft modification of silk fibroin material subjected to insoluble treatment; and (3) in-situ oxidation polymerization of thieno[3,4-b]-1,4-dioxin-2-methanol on silk fibroin material surface subjected to graft modification. The method can be used for preparing the silk fibroin conductive composite material with the polythieno[3,4-b]-1,4-dioxin-2-methanol grafted on the surface; the surface sheet resistance is 0.9*10<5>-5*10<7>ohm; the preparation technique is simple and mild; and the obtained novel biological material has high application value, and especially can be used as a nervous tissue engineering material and a neural restoration material.

A disclosed preparation method for a polylactic acid fiber three-dimensional bionic porous ordered scaffold comprises the following steps: slicing polylactic acid (PLA) with good biodegradability and biocompatibility, and successively performing spinning and winding to prepare fiber assemblies in parallel arrangement; using a medical binder to bond the loose and parallel-arranged PLA fiber assemblies to form a stable structure; and then curing and naturally cooling to room temperature. The polylactic acid fiber three-dimensional bionic porous ordered scaffold prepared by using the method has relatively small thickness and weight, relatively large pore diameter and porosity, and relatively good order degree, pore communication property, hydrophily, hydroscopicity, mechanical properties and adsorption capability on gelatin. The ordered scaffold has relatively good application prospect, is applicable to aspects of cell vectors of blood vessel engineering and nerve tissue engineering, ligament base materials, medicine loading and release, nerve regeneration of nerve tissue engineering, implantation type functional muscle, restoration of knee damage and bone arthritis, bone tissue engineering, and the like.

The invention discloses an oriented conductive collagen hydrogel, a biomimetic conductive nerve scaffold material and a preparation method of the oriented conductive collagen hydrogel and the biomimetic conductive nerve scaffold material. The mixture of natural biological macromolecular collagen and polymer nanoparticles with excellent biocompability is used as a raw material, and oriented conductive collagen hydrogel fiber is prepared by a coaxial microfluidic chip with a PEG (polyethylene glycol) buffer solution; the biomimetic conductive nerve scaffold material with in situ loading of cellsis obtained by adding the cells in the process of hydrogel preparation. The hydrogel fiber prepared has the advantages of electrical conductivity matching with natural nerve tissue, similar mechanical properties, good biocompatibility, and can be arranged along the direction of the hydrogel fiber on a micro and nano scale, and can simulate the directional structure in the natural nerve tissue; the biomimetic conductive nerve scaffold material prepared can stimulate the arrangement of neurous in the nerve tissue along the direction of the nerve fiber and conduction of electrical signals alongaxons, and shows a good application prospect in the field of neural tissue engineering.

A polypeptide scaffold for the nervous tissue engineering has the structure: C16H31O- Ala-Ala-Ala-Gly-Gly-Gly-Gly-Asp-Asp-Ile-lys-Val-Ala-Val-COOH. Its advantages are high visoelasticity, easy injection to target site, high degradability and biologic utilization rate, and low toxic by-effect and immunoreaction. Its preparing process and its application in preparing medicines are also disclosed.

The invention discloses preparation and application of a linear biodegradable polyester elastomer with a controllable elasticity and shape memory effect. The linear biodegradable polyester elastomer with the controllable elasticity and shape memory effect is formed by conducting copolymerization on a caprolactone monomer containing a lateral cyclic ether structural unit and caprolactone, wherein the molar ratio of the caprolactone monomer containing the lateral cyclic ether structural unit to the caprolactone is 5:95-25:75, and the structural formula can be found in description. The viscoelasticity of the linear biodegradable polyester elastomer with the controllable elasticity and shape memory effect is remarkably improved, the elongation at break can reach 1600% and above, the elastomer can be dissolved in a conventional organic solvent, a three-dimensional porous support can be constructed conveniently through a construction technology of the porous support such as, electrostatic spinning, three-dimensional printing and phase separation, tissue engineering blood vessel scaffold materials, myocardial patches, nervous tissue engineering scaffold materials and the like are prepared, and the preparation and application of the linear biodegradable polyester elastomer can be widely applied to the fields of soft tissue engineering scaffolds, tissue repair and regenerative medicine.

The invention discloses a preparation method of high-conductivity hollow porous composite fibers which take a conductive polymer as a shell and a degradable polymer as a core and have core-shell structures, and a dual-pore structure membrane of the high-conductivity hollow porous composite fibers as well as an application of the dual-pore structure membrane in tissue engineering. The preparation method comprises the steps of preparing the degradable polymer, hollow fibers with porous surfaces and the dual-pore structure membrane through an electrostatic spinning method, preparing a degradable polymer / conductive high polymer, hollow porous composite fibers and a dual-pore structure membrane thereof through an in-situ polymerization method, and cleaning and drying to obtain the high-conductivity hollow porous composite fibers with the core-shell structures and the dual-pore structure membrane of the high-conductivity hollow porous composite fibers. The high-conductivity composite micron / nano-fiber dual-pore structure membrane prepared by the invention can be applied to nerve tissue engineering, has the advantages of high conductivity, high porosity, large pore size and good chemical stability, and has a practical value in nerve tissue engineering, and the preparation method of the membrane is simple and feasible.

The invention discloses a method for preparing a novel magneto-induction degradable nervous tissue engineering material. The technical scheme of the method is carried out in the following steps: 1, preparing magnetic nanoparticles MNPs; 2, preparing a magnetic gel-shaped turbid liquid; 3, preparing a nervous tissue engineering material; and 4, carrying out crosslinking disinfection treatment. According to the magneto-induction degradable nervous tissue engineering material prepared by the method, a generated nerve grows in an oriented manner by the magnetic field inside the tissue material to accelerate repairing and the forming of myelin sheath, and the effect of repairing nerve defect can be further improved.

A degradable tubular high-molecular porous foam material used as the porous cell scaffold of blood vessel tissue or nerve tissue engineering is prepared from a high-molecular material through use of combined mould. Its advantages are high porosity up to 90% and uniform distribution of pores.

The invention discloses a method for directionally differentiating human iPSCs (induced pluripotent stem cells)-derived 3D (three-dimensional) retinas by means of induction to obtain retinal ganglion cells. The method includes digesting nerve fiber layers of the human iPSc-derived 3D retinas to obtain single cells; inoculating seed cells, namely the single cells, in induction differentiation culture media and cultivating the seed cells in the induction differentiation culture media; differentiating the seed cells to obtain the retinal ganglion cells. The induction differentiation culture media are culture media with neurotrophic factors and concentration-maintained retinal differentiation nutrient liquid. The method has the advantages that a novel functional RGCs (retinal ganglion cells) research approach is obtained from an angle of stem cell induction differentiation, a novel research idea is provided for directionally differentiating human iPSc-RGCs, a novel research clue is provided for investigating human iPSc-RGCs regulation mechanisms, a novel seed cell is provided for research on nerve tissue engineering, and theoretical and laboratory bases are laid for treating visual impairment by means of stem cell transplantation.

The invention discloses a three-dimensional active complex of dental pulp stem cells for nervous tissue engineering and a three-dimensional construction method thereof. The three-dimensional active complex and the three-dimensional construction method disclosed by the invention have the main beneficial effects that a GelMA-bFGF hydrogel complex is constructed by GelMA hydrogel carrying bFGF, and as an active carrier for three-dimensional culture of the dental pulp stem cells, the three-dimensional active complex can provide a supporting effect, an exchange effect and active microenvironment and other effects for culturing the dental pulp stem cells; a large amount of active dental pulp stem cells can be effectively obtained, the benefit for proliferation and fast growth of the dental pulp stem cells is achieved, and the active three-dimensional-culture dental pulp stem cells can be effectively obtained for application in the nervous-tissue engineering research.

The invention discloses a PCL (polycaprolactone) / CNTs (carbon nanotubes) compound electrospun film with optimal fiber orientation for inducing nerve regeneration. The PCL / CNTs compound electrospun film has the characteristics of large specific surface area and high porosity, is very similar to an extracellular matrix, and can be used as a support for supporting regeneration of various cells into complete tissues. The introduction of CNTs can further enhance the electrical activity of the composite support, so that the repair efficiency of nervous tissues is further improved under the action ofelectrical stimulation. The obtained PCL / CNTs compound electrospun film with the optimal fiber orientation lays a solid theoretical foundation for nerve defect repair in nervous tissue engineering, the process is simple, convenient, and easy to operate, and popularization and application are facilitated.

The invention discloses a choline polysaccharide conductive nervous tissue engineering hydrogel material as well as a preparation method and the application thereof, and relates to conductive nervous tissue engineering polysaccharide hydrogel and a preparation method thereof. The invention aims to solve the problem that the existing conductive nervous tissue engineering hydrogel material cannot give consideration to excellent conductivity and biological signal transduction functions, so that the conductive nervous tissue engineering hydrogel material is poor in biological signal transduction capability. The preparation method comprises the following steps: 1, preparing carboxymethyl chitosan; 2, preparing catechol carboxymethyl chitosan; 3, preparing choline-catechol carboxymethyl chitosan; and 4, preparing the choline polysaccharide conductive nerve tissue engineering hydrogel material. The choline polysaccharide conductive nervous tissue engineering hydrogel material is used as a nervous tissue engineering material for promoting defective nerve repair. The choline polysaccharide conductive nerve tissue engineering hydrogel material can be obtained.

The invention discloses a retina cell scaffold biological surgical binder and a preparing method thereof. The retina cell scaffold biological surgical binder comprises laminin, type-IV collagen, nidogen, human heparin sulphate protoglycans, fibroblast growth factors, matrix metalloproteinase and phosphate buffer salt solution. By the adoption of the retina cell scaffold biological surgical binder, degradable human iPSCs-derived retina nerve scaffold (iRMP) can be bound with a host retina nerve layer, and effective integration of a cell biological scaffold and a host retina is guaranteed. The tissue engineering technology and clinical practice are combined, and the retina cell scaffold biological surgical binder has certain theoretical and practical application value for regenerative medicine fundamental research and ophthalmology clinical treatment, promotes optic nerve system damage repair and nervous tissue engineering research, and lays a theoretical and experimental foundation for tissue engineering transplantation therapy of visual damage.

The invention discloses a method for recycling waste light disk and a method for culturing PC12 cells by virtue of the recycled light disk. According to the methods, electronic waste is directly utilized and is applied to nervous tissue engineering, so that ready-made waste materials with various micron / nanometer structures can be provided so as to solve problems in the supplying and manufacturing of micron / nanometer patterns, and a new hopeful environmental protection manner can be provided; the application proves that wasted electronic products can be directly and repeated used as highly anisotropic substrates in the in vitro study of cell behaviors; PC12 cells cultured on the two substrates with different morphologies present different cell morphologies; and more chances are provided for the combination of theories and advantages of the information technique field and other fields, and a novel biological interface is hopefully constructed and designed by combining methods of biochemical building blocks, projects, materials and the like and is applied to the fields of biomedicines, biosensors, energy and the like.

The invention discloses a keratin fiber-nerve growth factor (NGF) composite stent of nervous tissue engineering and a preparation method thereof. The preparation method for the keratin fiber-nerve growth factor composite stent of the nervous tissue engineering comprises the following steps: processing keratin fibers by using acidic solution, breaking an epidermal layer on the topmost surface, andexposing an internal cortical layer, to obtain the processed keratin fibers; enabling a nerve growth factor to be loaded on the processed keratin fibers, to obtain the keratin fiber-nerve growth factor composite stent. The provided composite stent can be used for remarkably promoting the survival of neural stem cells and increasing a proportion of the neural stem cells differentiated as nerve cells and an expression number of acceptors Trk A. So the NGF-keratin fiber can be used as a novel three-dimensional stent. The composite stent is capable of providing a new method and idea for treating nervous system injuries or diseases by using a nervous tissue engineering method.

The invention relates to a method for constructing three-dimensional engineered nervous tissue, the three-dimensional engineered nervous tissue and an application thereof. Process. The method comprises the following steps: 1, carrying out isolated culture on animal neural stem cells; 2, applying a physiological electric field; subculturing the neural stem cells isolated and cultured in the step 1to the fifth generation, centrifuging neural spheres with a diameter of 100-200 microns for later use, pre-melting matrix gel Matrigel on ice for later use, and continuously applying a physiological electric field to stimulate for 7-14 days through physiological electric field application equipment for 1-2 hours every day; 3, optimizing a culture solution suitable for engineering nerve tissue growth: adding 1 / 50 of B27, 1 / 100 of N2, 10-20 ng / ml of bFGF and 1% of fetal calf serum into DMEM / F12; and 4, applying the physiological electric field for 7-14 days to obtain the three-dimensional engineered nervous tissue. The technical problems that in the application process of neural tissue engineering, the number of neuronal differentiation of existing neural stem cells is extremely small, and neurite cannot be induced to grow orderly in a three-dimensional growth environment to form a developed neural network are solved.

The invention belongs to the technical field of medical material preparation, and provides a preparation method of a degradable piezoelectric fiber stent and a product thereof. The preparation method of the degradable piezoelectric fiber stent comprises the following steps: preparing a gelatin solution, performing electrostatic spinning to prepare a fiber membrane, and drying to obtain a gelatin fiber membrane; placing the gelatin fiber membrane in glutaraldehyde saturated steam for normal-temperature crosslinking, and drying to obtain the degradable piezoelectric fiber stent. The preparation method provided by the invention adopts electrostatic spinning and chemical crosslinking modes for preparation, the method is simple, and reaction conditions are easy to realize. The prepared degradable piezoelectric fiber membrane stent can induce neural differentiation of neural stem cells in a wireless stimulation mode, and has important significance in clinical and nervous tissue engineering.