Nervous tissue engineering fibrous membrane and preparation method

Abstract

The invention discloses a nervous tissue engineering fibrous membrane and a preparation method. The method comprises the following steps of: dissolving a high molecular material into water or an organic solvent to prepare electrostatic spinning solution; injecting the high molecular solution into an injector; performing electro-spinning to form nanofibers, which are arranged tidily, to prepare a high molecular fibrous membrane; and immersing the high molecular fibrous membrane into pyrrole aqueous solution to form core-sheath fibers. In the preparation method of the nervous tissue engineering fibrous membrane provided by the invention, the fibrous membrane is constituted by conductive core-shell nanofibers; the core is high molecule which has excellent material biocompatibility; the nanofibers are arranged tidily by controlling the preparation conditions; the shell-layer material is polypyrrole; the biocompatibility is good; the prepared core-sheath fiber can provide a unique platform to grow and extend neurites; and the process is simple, easy to operate and low in cost and has broad application prospect in the field of nervous tissue engineering.

Description

Technical field: [0001] The invention relates to a nerve tissue engineering fiber membrane, in particular to a fiber membrane with synergistic effects of nerve morphology and electrical stimulation and a preparation method thereof. Background technique: [0002] The ultimate goal of neural tissue engineering is to spontaneously exhibit biochemical, morphological, and electrical properties of neural tissue. However, in most previous studies, the effects of morphology and electrical stimulation, or biochemical properties on synapses, have been examined separately; it has been demonstrated that dorsal root nerves can be controlled using aligned electrospun fibers as templates. The direction of synapse growth in DRG synapses, and even guide axon growth and glial cell migration; Recently, it has been reported that embryonic stem cells can be differentiated into neurons on poly(E-caprolactone) fibers (PCL), and with Compared with random fibers, neatly aligned fibers can improve t...

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Problems solved by technology

[0003] However, there are few studies on the electrical and morphological synergistic effects of guiding and expanding neurons. Some studies have made polypyrrole (PPY) microchannels on sheets covered with indium tin oxide (ITO) by etching techniques, and studied How hippocampal cells of embryonic rats respond to combined electrical and morphological signals. They studied the guiding effect of polypyrrole microchannels with a width of 1 and 2 mm on neurite outgrowth, but the results were not satisfactory because of insufficient spatial resolution; Another study prepared submicron coaxial fibers by depositing polypyrrole on poly(methyl methacrylate) (PMMA) fibers, but did not apply this fiber to the study of synapse growth