Three-dimensional active complex of dental pulp stem cells for nervous tissue engineering and three-dimensional construction method thereof

Abstract

The invention discloses a three-dimensional active complex of dental pulp stem cells for nervous tissue engineering and a three-dimensional construction method thereof. The three-dimensional active complex and the three-dimensional construction method disclosed by the invention have the main beneficial effects that a GelMA-bFGF hydrogel complex is constructed by GelMA hydrogel carrying bFGF, and as an active carrier for three-dimensional culture of the dental pulp stem cells, the three-dimensional active complex can provide a supporting effect, an exchange effect and active microenvironment and other effects for culturing the dental pulp stem cells; a large amount of active dental pulp stem cells can be effectively obtained, the benefit for proliferation and fast growth of the dental pulp stem cells is achieved, and the active three-dimensional-culture dental pulp stem cells can be effectively obtained for application in the nervous-tissue engineering research.

Description

technical field [0001] The invention specifically relates to the technical field of stem cell culture, in particular to a three-dimensional active complex of dental pulp stem cells for neural tissue engineering and a three-dimensional construction method thereof. Background technique [0002] Peripheral nerve injury (Peripheral nerve injury, PNI) is a common clinical traumatic disease, the incidence of PNI is high worldwide, and the proportion of patients with complete neurological recovery after clinical systemic treatment is less than 50% , seriously affecting the daily life ability of patients, causing a sharp decline in the quality of life of patients, and bringing great economic burdens to society, families and individuals. At present, the clinical treatment of PNI is mainly autologous nerve transplantation, but the source of autologous nerve is limited, and it is often accompanied by functional impairment of the donor site, and is also limited by problems such as repai...

AI Technical Summary

Problems solved by technology

Among them, the seed cells traditionally used for neural tissue engineering are Schwann cells (Schwann cells, SCs), but SCs are terminally differentiated cells with limited proliferation ability; subsequently, embryonic stem cells (embryonic stem cells, ESCs) and bone marrow Stromal stem cells (Mesenchymal stem cells, MSCs) are used as seed cells for neural tissue engineering, but there are issues such as ethics and limited selection of materials, and their clinical application is limited to a certain extent.

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