Keratin Fiber-Nerve Growth Factor Composite Scaffold for Nerve Tissue Engineering

Abstract

The invention discloses a keratin fiber-nerve growth factor (NGF) composite stent of nervous tissue engineering and a preparation method thereof. The preparation method for the keratin fiber-nerve growth factor composite stent of the nervous tissue engineering comprises the following steps: processing keratin fibers by using acidic solution, breaking an epidermal layer on the topmost surface, andexposing an internal cortical layer, to obtain the processed keratin fibers; enabling a nerve growth factor to be loaded on the processed keratin fibers, to obtain the keratin fiber-nerve growth factor composite stent. The provided composite stent can be used for remarkably promoting the survival of neural stem cells and increasing a proportion of the neural stem cells differentiated as nerve cells and an expression number of acceptors Trk A. So the NGF-keratin fiber can be used as a novel three-dimensional stent. The composite stent is capable of providing a new method and idea for treating nervous system injuries or diseases by using a nervous tissue engineering method.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a nerve tissue engineered keratin fiber-nerve growth factor composite scaffold and a preparation method thereof. Background technique [0002] The existence of neurotrophic factors can maintain the survival and migration of neural stem cells in vitro, so that they have the potential to differentiate into neurons, astrocytes and oligodendrocytes. Studies have shown that neurotrophic factors can promote the repair of spinal cord injuries [Li X, Yang Z, Zhang A. The effect of neurotrophin‐3 / chitosancarriers on the proliferation and differentiation of neural stem cells. [J]. Biomaterials, 2009, 30 (28):4978.]. Nerve growth factor (NGF) was the first neurotrophic factor discovered. Nerve growth factor can promote axon growth[]Mccormick A M, Jarmusik N A, Leipzig N D. Co‐immobilization of semaphorin3A and nerve growth factor to guide and pattern axons.[J].Acta Biomaterialia,2015,28:33‐44.]...

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Problems solved by technology

However, these studies have focused on extracting keratin from hair, and then preparing and chemically modifying materials, such as preparing thin films [Zhong Y, Bellamkonda R V. Biomaterials for the central nervous system. [J]. Journal of the Royal Society Interface,2008,5(26):957‐975.], electrospun nanofibers [Jain A, KimY T, Mckeon R J, et al.In situ gelling hydrogels for conformal repair of spinal cord defects, and local delivery of BDNF after spinal cord injury.[J].Biomaterials,2006,27(3):497‐504.Woerly S,Petrov P,SykováE,et al.Neuraltissue formation within porous hydrogels implanted in brain and spinal cordlesions:ultrastructural,immunohistochemical, and diffusion studies.[J].Tissue Engineering,1999,5(5):467‐488.], hydrogel[]Meilander N J, Pasumarthy M K, Kowalczyk T H, et al.Sustained release of plasmad DNA using lipid microtubulesand agarose hydrogel .[J].Journal of Controlled Release,2003,88(2):321.], while ignoring the research on the biological effects of hair itself, a natural keratin fiber with a fine structure

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